Application Note
Conjugation CleanAb Kits
Efficient Removal of Oligonucleotides, Dyes, and other Conjugation Reagents from Conjugated Antibodies
Non visible textResearch Area
Overcoming FGF-10 Instability in Cell and Organoid Cultures
Written by Matthew Ross, PhD, Senior Scientist at Proteintech
Non visible textResearch Area
Mastering the Transition: A Protocol for Differentiating SH-SY5Y Cells into Neurons
Written by Ceanne Elliott, PhD Candidate at Indiana University School of Medicine
Non visible textResearch Area
Industry First: Proteintech Launches IHC Whole Slide Image (WSI) Viewer
Written by: Abel Zhang at Proteintech
Non visible textTestimonial
Scientist Stories: Dr Joe McKellar on Imaging the Cytoskeletal Architecture of LUHMES-Derived Neurons
Dr Joe McKellar shows us what a neuron's skeleton really looks like.
Non visible textTestimonial
Scientist Stories: Dr Joe McKellar on Septins, the Understudied Fourth Component of the Cytoskeleton
The fourth component of the cytoskeleton, and why it deserves more attention
Non visible textResearch Area
Microscopy & imaging guide for researchers: From experimental setup to image acquisition
Written by: Sadaf Fazeli, PhD in Hematology at Karolinska Institutet
Non visible textTestimonial
Scientist Stories: Dr Joe McKellar on Imaging Influenza A
How Dr Joe McKellar uses Proteintech antibodies in confocal microscopy to study viral infections
Non visible textResearch Area
The Unexpected Cell Type Behind Heart Attacks and Strokes
Written by Sofija Sundman, PhD candidate
Non visible textResearch Area
Midbrain organoid generation: Practical tips from the bench
Written by: Jamuna Karanankattil Sukumaran, Senior Scientist at Protientech
Non visible textResearch Area
A Practical Guide for Dendritic Cell Identification by Flow Cytometry
Written by: Sepideh Jahangiri, PhD in Experimental Medicine at Université de Montréal
Non visible textResearch Area
Streamlining Multiplex Imaging in Expanded Tissues with FlexAble 2.0 Antibody Labeling Kits and Magnify™ Expansion Microscopy
Authors: Aleksandra Klimas, Tina Ryu, and Yongxin (Leon) Zhao, Magnify Biosciences, Inc.
Non visible textResearch Area
A Practical Step-by-Step Guide to Subcellular Fractionation in Mammalian Cells
By Alexandra-Teodora Szabo, Postgraduate Researcher in Mitochondrial Biology at University of Tübingen
Non visible textResearch Area
Cardiac Organoids in Research
Written By Anna Katharina Schrattel – University Hospital of Heidelberg, University of Heidelberg
Non visible textResearch Area
Tertiary Lymphoid Structures: What They Are and Why They Matter in Immuno-oncology
Written by Sarah Wilkey, PhD Student at the University of Queensland, Australia
Non visible textResearch Area
PhD Data Management Guide: How to Organize, Label, and Store Research Data Effectively
Written by Sara Bitar, PhD researcher at Johannes Gutenberg University of Mainz, Germany
Non visible textResearch Area
Guardians of the gut: Gamma-delta T cells and their emerging role in the gut-immune-brain axis
Written by Rogério Rocha de Castro, PhD candidate in Medical Neuroscience at Radboud University Medical Center
Non visible textTestimonial
Scientist Stories: Dr Clarisse Brunet on Reliable Antibodies for Studying Human Cortical Development
How Dr Clarisse Brunet uses Proteintech antibodies to achieve reliable, reproducible cell fate analysis in human neurodevelopment research.
Non visible textResearch Area
A Guide to Markers for Oligodendrocyte Identification
Written by Tessa Dallo, PhD student at Florida Atlantic University
Non visible textTestimonial
Scientist Stories: Dr Rebecca Saleeb on Multiplex Imaging Possibilities with Secondary Nanobody Technology
How Dr Saleeb is using Proteintech antibodies and nanobodies to unlock new flexibility in multiplexed immunofluorescence for ALS
Non visible textCareer Development
Beyond The Lab: Real Scientist Stories
Written by Alexandra-Teodora Szabo, PhD student in Mitochondrial Biogenesis at University of Tübingen
Non visible textResearch Area
You Shall Not Pass! - The Blood-Brain Barrier
Beatriz Achón Buil, PhD candidate in Neuroscience, Institute for Regenerative Medicine (University of Zürich)
Non visible textResearch Area
Microglia – An Update on How to Define this Multifaceted Cell Population
Written by Maria-Luisa Wiesinger; PhD student in pediatric low-grade glioma research at the German Cancer Research Center, Heidelberg
Non visible textTestimonial
Scientist Stories: Charlotte Scholtes on Unlocking ER–Mitochondria Contacts with Proteintech Antibodies
How Charlotte Scholtes, PhD used Proteintech antibodies to accelerate research into ER–mitochondria contacts and metabolic regulation.
Non visible textTestimonial
Scientist Stories: Revealing BAG3’s Role in Tau Clearance with Proteintech Antibodies
Written by Hayley Wnuk, PhD, Post-Doctoral Research Associate at the University of Rochester Medical Center
Non visible textResearch Area
The power of neutrophils: How to identify the immune system’s first responders
By Maria Alejandra Feliz Norberto, M.S., PhD Candidate at Albert Einstein College of Medicine
Non visible textResearch Area
AI in Neuroscience: Science-Fiction or Reality?
Written by Daniel Doyon, PhD student at Karolinska Institutet in Stockholm
Non visible textResearch Area
Tips on your coding journey as a PhD student
Written by María Bueno Álvez, PhD candidate at Science for Life Laboratory
Non visible textTestimonial
Scientist Stories: Fabienne Bejjani, PhD on Improving Research Efficiency with Proteintech Antibodies
Exploring how high-quality, fast-delivery antibodies from Proteintech enhanced experimental accuracy and reproducibility in molecular biology research.
Non visible textTestimonial
Scientist Stories: Léa Pechtimaldjian on Advancing 3D Vascular Imaging with FlexAble Technology
How Léa Pechtimaldjian used Proteintech antibodies and FlexAble technology to accelerate 3D vascular imaging.
Non visible textResearch Area
The Gut Microbiome and Depression: Current Insights and Future Directions
Written by Hasnain Methiwala, PhD Student at the University of Minnesota Medical School
Non visible textResearch Area
Immunofluorescence Imaging of Live and Fixed Pancreatic Tissue Slices Using FlexAble 2.0 Antibody Labeling
Written by Vijay Sai Josyula, Ph.D. Candidate at Uppsala University
Non visible textTestimonial
Research Grant Stories: Dr Elisa Damo on Mapping Antibody Distribution in Pain Models
How Proteintech Research Grants allow researchers to advance their projects. #InTheLabWithProteintech
Non visible textResearch Area
Endothelial Cells: Gatekeepers in HIV Disease Progression
Written by Bryant Avalos, PhD, Postdoctoral Researcher at University of California, San Diego
Non visible textResearch Area
How Organ-Chip Technology is Redefining Human Physiology and Disease Modelling
Written by Catrin Bevan, PhD Student at Queen Mary University of London.
Non visible textResearch Area
Hematopoietic Stem and Progenitor Cells (HSPCs): What They Are and How to Identify Them
Written by Maria Alejandra Feliz Norberto, M.S., PhD Candidate at Albert Einstein College of Medicine
Non visible textResearch Area
What is Optogenetics? How Scientists are Using Light to Understand the Brain
Written by Anastasiia Caangay, PhD candidate at the University of Alabama-Birmingham
Non visible textCareer Development
Imposter Syndrome in Scientific Research: The Hidden Epidemic
Written by Hannah Joanne Lee, PhD Candidate at Queen Mary University of London
Non visible textResearch Area
How to Write a Strong Statement of Purpose for a PhD Program
Written by Breanna Lam, PhD Candidate at Massachusetts Institute of Technology
Non visible textResearch Area
Get the Most from Your iPSCs: Improved Cardiomyocyte Differentiation
Written by Jamuna Karanankattil Sukumaran, Senior Scientist at Proteintech
Non visible textResearch Area
Targeting Purinergic Signaling: A New Frontier in Cancer Immunotherapy
Written by Sepideh Jahangiri, PhD, University of Montreal
Non visible textResearch Area
Markers for Astrocyte Identification
Written by Haddie DeHart, PhD student at Indiana University School of Medicine
Non visible textResearch Area
Ferritinophagy: Understanding Its Roles in Iron Homeostasis and Disease
Written by Elizabeth Byers, PhD Candidate at The Pennsylvania State University
Non visible textResearch Area
The Heart of the Matter: Voltage-Gated Calcium Channels in Cardiovascular Disease
Written by Hannah Joanne Lee, PhD Candidate at Queen Mary University of London
Non visible textResearch Area
Commanding Endosomal Sorting: The Role of the Commander Complex in Protein Trafficking
Written by Kincaid Ingram; PhD researcher focusing on endosomal biology in health and disease at the University of Bristol, UK
Non visible textApplication Note
The importance of epitope selection in experimental design
Discover how pinpointing the right antibody binding site can transform the reliability and impact of your research, diagnostics, and therapeutic strategies.
Non visible textResearch Area
Every protein tells a story
Unveiling the story of proteins—One epitope at a time
Non visible textApplication Note
Identifying Cortical Cell Types Through Immunofluorescence and Electrophysiology
Written by Airi Watanabe, Ph.D. Candidate at McGill University
Non visible textCareer Development
Everything is an Opportunity: A science communication interview with Professor Manasi Nandi
Written by Amanda Bowman, PhD Candidate at King’s College of London
Non visible textResearch Area
Clopidogrel Dual Antiplatelet Therapy and Novel Treatments for Atherothrombosis
Written by Shaden Salimi Graduate Student at McGill University
Non visible textResearch Area
A Guide to Studying Wild-Type and Mutant Proteins
Written by Suparba Roy, PhD Candidate at Université Laval
Non visible textResearch Area
Antibody Validation for ELISA: Ensuring Accurate and Reliable Results
Written by Nerea Gómez de San José, PhD in protein biomarker research.
Non visible textCareer Development
How To Write a Literature Review: A Comprehensive Guide
Written by Dr. Reshma Patil
Non visible textResearch Area
Immune Checkpoint Inhibitor Therapy: Revolutionizing Cancer Treatment
Written by Sepideh Jahangiri, PhD Candidate at the University of Montreal
Non visible textResearch Area
Understanding FGF Signaling: A Key Pathway in Development and Disease
Written by Jaime Fernández Sobaberas, 3rd year PhD student in Biochemistry at Heidelberg University, Germany.
Non visible textApplication Note
The FlexAble way to improve your immunofluorescence with IBEX
Written by Aanandita Kothurkar and Natalia Jaroszynska from UCL Institute of Ophthalmology
Non visible textApplication Note
A Guide to Building a Direct Sandwich ELISA
Written by Dr. Reshma Patil
Non visible textApplication Note
A Guide to Immunostaining the Cerebellum
Written by Sophia Leung, PhD, Postdoctoral Fellow at McGill University
Non visible textResearch Area
Cuproptosis in Health and Disease: Copper’s Double-Edged Sword
Written by Jeyashree Alagarsamy, PhD Candidate at the University of Cincinnati
Non visible textApplication Note
Mimicking the Extracellular Matrix Through Electrospinning
Written by Elizabeth Byers, PhD Candidate at The Pennsylvania State University
Non visible textResearch Area
Nanobodies in Structural Biology
Written by George Biggin, Structural Biology PhD Student at The University of Manchester
Non visible textResearch Area
Understanding Liver Cancer: Causes, diagnostics, and therapeutic avenues
Written by Selin Dayan-Özdurmus, PhD researcher at German Cancer Research Center (DKFZ), Heidelberg
Non visible textResearch Area
Protein Phosphorylation; Not just a binary switch.
Written by Joris Frenz; Proteomics and Cancer cell signaling PhD researcher at the German Cancer Research Center, Heidelberg
Non visible textApplication Note
Immunofluorescence techniques in zebrafish retina
Written by Natalia Jaroszynska, PhD Candidate at UCL Institute of Ophthalmology
Non visible textResearch Area
Navigating Nematodes: Tips for Successful Worm Picking
Written by Levon Tokmakjian, PhD Candidate at University of Toronto
Non visible textCareer Development
5 creative ways to stay productive in graduate school
Written by Sophia Leung, PhD from the University of McGill
Non visible textResearch Area
What is Macrophage Polarization?
Written by Consuelo Coser, PhD Student at University of Nottingham
Non visible textCareer Development
How to transition from academia to industry using LinkedIn
Written by Elena Hoffer, PhD. Co-Founder and CEO of Alma.me.
Non visible textResearch Area
Unlocking the Secrets of Aging: The Role of Epigenetics in Cellular Senescence
Written by Shiksha Dutta, PhD student at University of Montreal
Non visible textCareer Development
How to create a LinkedIn profile for scientists
Discover how to optimize your LinkedIn profile to promote yourself and your science.
Non visible textApplication Note
Guide to Flow Cytometry Panel Building
Written by Dr. Frédéric Duval and Sari Gezzar-Dandashi from the Maisonneuve-Rosemont Hospital Research Center.
Non visible textApplication Note
How to choose the right secondary antibody?
How to choose the best secondary antibody for your application.
Non visible textResearch Area
PLSCR1: An exciting new defender against COVID-19
Written by Sophie Williams; Biochemistry PhD researcher at the University of Bristol.
Non visible textResearch Area
How to Better Study Ubiquitination
Understand the role of Ubiquitin in protein degradation and learn how ChromoTek’s Ubiquitin Trap can help you overcome certain ubiquitin-related research challenges.
Non visible textApplication note
Quartz Crystal Microbalance: Next-generation biosensor?
Understand how the Quartz Crystal Microbalance technique could be the future of biosensing and biomarker detection.
Non visible textResearch Area
How to culture mouse primary cerebral microvascular endothelial cells
Learn to how to isolate and culture these integral cells types for blood brain barrier research.
Non visible textResearch Area
m5C Modifications in RNA and Cancer
Explore how the epigenetic modification m5C regulates the fate of RNA molecules and cancer prognosis.
Non visible textResearch Area
Decoding the Mitochondrial Unfolded Protein Response
Summary of a Nature paper from Goethe University Frankfurt revealing the previously unknown mechanisms of the mitochondrial unfolded protein response.
Non visible textResearch Area
Tips and tricks for iPSC culture
Top tips for successful induced pluripotent stem cell and embryonic stem cell culture.
Non visible textResearch Area
A Guide to Staining Organelles
Identifying subcellular structures is key for mapping the localization of target proteins. This blog features key markers to identify organelles by immunofluorescence.
Non visible textApplication Note
Under the hood of t-SNE – the t-SNE algorithm explained
Walk step-by-step through how t-SNE represents your high-dimensional data in two dimensions.
Non visible textCareer Development
8 Tips for Networking at Scientific Conferences
Here are our top tips on how to use conferences to make meaningful connections.
Non visible textResearch Area
B cell mitochondria: much more than a powerhouse
Nature Immunology paper from a team the University of Oxford detail how mitochondria are essential for B cell development and lymphomagenesis.
Non visible textResearch area
Biomarkers for neurodegenerative disorders
Relevant fluid biomarkers are required to enable early and accurate diagnosis of neurodegenerative diseases. This blog details current biomarkers and promising future biomarkers.
Non visible textApplication Note
Introduction to t-SNE for Flow Cytometry
Understand the pros and cons of t-SNE, an algorithm for visualizing high-dimensional data
Non visible textEarly-Career Researchers
Time management and self-care in research
Tips and tricks to help improve efficiency and personal care for early career researchers.
Non visible textApplication note
Key considerations for raw material selection in cell therapy manufacturing
Learn more about regulatory requirements and best practices in sourcing raw materials for cell based therapy manufacturing
Non visible textResearch area
Scientists Discover Dynein-Driven Motility in Primary Cilia!
Researchers have discovered that the movement of primary cilia on pancreatic cells assists in insulin secretion
Non visible textResearch area
Exposure to microplastics increases risk of liver fibrosis
Written by Ryan Murray, PhD Student at the University of Leeds
Non visible textApplication Note
RIP/RIP-seq to study RNA Modifications
Written by Sripriya Raja, PhD Candidate at University of Pittsburgh.
Non visible textResearch Area
The Role of Autophagy in Cancer
Written by Dharaniya Sakthivel, PhD student at Baylor College of Medicine.
Non visible textResearch Area
Novel organoid model enables study of microglia in health and disease
Scientists develop a new human brain organoid to include immune cells, making it more realistic for physiological and pathological research.
Non visible textCareer development
How to Review a Scientific Paper in 10 Easy Steps
Written by Jaime Fernández Sobaberas, 3rd year PhD student in Biochemistry at Heidelberg University, Germany.
Non visible textCareer development
How to make a scientific conference poster
Our top tips to make an impactful poster at your next conference
Non visible textResearch area
Scientists Validate Biomarker Assay for Parkinson’s Disease!
A clinical study shows that α-synuclein seed amplification assays (SAAs) can reliably identify people with Parkinson’s disease.
Non visible textApplication Note
Go with the Flow - Make the most of this powerful technique
Discover all the ways flow cytometry can be used to take your research further
Non visible textCareer development
The Importance of Self-Care While Doing your PhD
by Rachel Surratt, MA, Associate Professional Clinical Counselor at Resiliency and Health Institute, LLC
Non visible textApplication note
Protein tags: Advantages and disadvantages
Understand when it is beneficial to tag a protein and when it is better to stay tag free.
Non visible textApplication Note
Spectral Compensation in Flow Cytometry
Excerpt from Mastering Flow Cytometry: Best Practices and Tips webinar given by Dr. Shweta Puntambekar, Ph.D. This webinar excerpt has been edited for clarity.
Non visible textCareer development
How to Navigate Finishing Your Ph.D. Remotely
Written by Sepideh Dadkhah, Ph.D. Candidate at the University of Kentucky
Non visible textResearch Area
Mitochondrial respiratory supercomplexes: a focus on COX7A isoforms
COX7A isoforms adapt the cellular energy production machinery to metabolic demands.
Non visible textCareer Development
How to write a PhD thesis: 10 top tips
Written by Kathryn Green, Final Year Ph.D. Student at the University of Nottingham.
Non visible textResearch Area
The role of ferroptosis in human diseases
Written by Dharaniya Sakthivel, PhD student at Baylor College of Medicine
Non visible textApplication note
Flow Cytometry Gating for Beginners
Written by Michelle Belmont, Scientist, MS at Proteintech Group.
Non visible textApplication Note
ChatGPT and BioGPT as tools for life science research
Written by Benjamin Raven, PhD student researching cellular senescence at the University of Sheffield, UK.
Non visible textApplication Note
Detecting RNA Methylation by Dot Blotting
A step-by-step guide by Proteintech scientists!
Non visible textResearch Area
New avenue for treatment of ALS and frontotemporal dementia? A canonical m6A reader is a potential new target for ALS and frontotemporal dementia
Non visible textResearch area
Introduction to cerebral organoids in research
Understand how brain organoids are made and why they are tipped to revolutionize life science research models.
Non visible textResearch area
Long-Term Effects of COVID-19 on the Gastrointestinal System
Written by Rahul Panchal, Scientist II at Proteintech Group in Chicago, IL.
Non visible textResearch Area
Vascular dysfunction in Alzheimer’s Disease
Written by Katy Walsh, PhD student researching vascular dementia at the University of Manchester, UK.
Non visible textCareer development
Women in Science
International Day of Women and Girls in Science
Non visible textApplication Note
Tips for detecting phosphoproteins by western blot
Tips and tricks to optimize your western blot for phosphoprotein detection
Non visible textResearch Area
Science paper increases understanding of how ALS and frontotemporal dementia pathology interact
Take a closer look at recent Science paper by Shao et al
Non visible textApplication Note
International Unit-based Specific Activity Determination for HumanKine® Products
Recombinant protein biological activity can be quantified by international units (IU) based on comparisons to a centralized set of standards
Non visible textResearch Area
What is the difference between pyroptosis and apoptosis?
Written by Mehak Passi, PhD Researcher at LMU Munich University
Non visible textApplication Note
Reverse Phase Protein Array – a high throughput proteomic tool
Discover the potential of RPPA and its many advantages over other proteomic techniques
Non visible textApplication Note
How to culture Bone Marrow Derived Macrophages
Our top tips to successfully extract and culture murine Bone Marrow Derived Macrophages
Non visible textResearch area
Breast Cancer Awareness Month
October is an annual health campaign to promote early detection, reduce risk factors and support patients
Non visible textCareer Development
How to start your own business after a PhD: an insider’s perspective
Looking to move from academia to industry? Get advice from Dr. Chris Bullock, CEO and Co-Founder of QV Bioelectronics, on how to become an entrepreneur after finishing your PhD.
Non visible textApplication Note
How to Identify Activated Microglia
Proteintech experts share ways to quantify your microglial cell population
Non visible textCareer Development
Building research careers in a post-COVID world
Creative strategies used by early-career researchers during the pandemic to establish themselves could launch careers after the pandemic fades.
Non visible textLab Life
How to prepare for a PhD viva - Ten tips for success
Written by Tiago Marcos, PhD, University of Edinburgh.
Non visible textApplication Note
7 Tips to successfully culture primary rodent neurons
Proteintech experts provide seven tips for improving the integrity of your primary neuronal cultures.
Non visible textResearch Area
Overview of the vascular system and the importance of endothelial cells
Written by Kathryn Green, PhD student at The University of Nottingham.
Non visible textCareer Development
Proteintech interview with an early-career researcher: Odetta Antico
Odetta Anitco from the University of Dundee, Scotland, speaks to Proteintech about her scientific career so far, her recent paper in Science Advances, and how to handle setbacks in research.
Non visible textResearch area
Replication Race: Scientists unravel why Omicron spreads faster
Written by Tiago Marcos Ph.D, guest author from the University of Edinburgh.
Non visible textResearch Area
Explore cytokeratin dynamics with CoraLite conjugates
Want to learn more about Cytokeratins and their role in disease development? Introducing CoraLite conjugated antibodies against cytokeratins.
Non visible textApplication Note
GFP-Booster: GFP Nanobody for better images in immunofluorescence
Higher resolution and better tissue penetration
Non visible textApplication Note
How to immunoprecipitate Flag®-tagged proteins
Flag®-tag (or DYKDDDDK-tag) is a commonly used short peptide tag for multiple applications such as immunoprecipitation (IP), protein purification, immunofluorescence, and Western blotting (WB).
Non visible textApplication Note
Why is the Fab-Trap™ so Fab-ulous?
ChromoTek’s Fab-Trap™ is an IP reagent based on an antibody Fab-fragment.
Non visible textApplication Note
Advantages and limitations of different antibody formats in immunoprecipitation
Non visible textApplication Note
Detecting low abundance proteins via Western Blot
Detecting low abundance proteins can be a challenge - here's how to optimize your Western Blot.
Non visible textApplication Note
7 Tech Tips For Successful ChIP Experiments
New to ChIP or just want to improve? Our tech tips will help you achieve successful ChIP experiments
Non visible textResearch Area
How Nanobodies could advance Sars-CoV diagnostics
Teresa Wagner and Ulrich Rothbauer discuss the development of Nanobodies against SARS-CoV-2 and why Nanobodies are versatile alternatives to classical antibodies in SARS-CoV-2 research.
Non visible textBlog
The highly adaptive cytoskeleton
The cytoskeleton is a highly important structure present in the cytoplasm of all cells. Learn how this is integral for many cellular functions, and the many ways researchers can study the cytoskeleton in our blog.
Non visible textApplication Note
How to conduct a Co-immunoprecipitation (Co-IP)
Learn how to perform Co-IP to study protein-protein interactions with this step-by-step guide
Non visible textApplication Note
Co-immunoprecipitation troubleshooting, tips and tricks
Co-immunoprecipitation (Co-IP) describes the isolation of a protein and its binding partners from a cell extract using a Nanobody or antibody that is bound to beads. The protein, that directly interacts with the Nanobody, or antibody beads is called “bait”. The binding partner that is indirectly precipitated is called “prey”.
Non visible textApplication Note
Mouse-on-mouse for Immunohistochemistry: tips and tricks
Learn how to mitigate issues with high background staining and perform mouse-on-mouse staining with confidence.
Non visible textApplication Note
Want to upgrade your immunofluorescence workflow? Go Direct!
Learn about the advantages of using directly conjugated primary antibodies for IF
Non visible textApplication Note
Flag-tag and 3x Flag-tag
Overview about specifications, properties, origin, sequence, size, specifications, etc. of Flag-tag
Non visible textResearch Area
How to choose your recombinant proteins, cytokines & growth-factors
Considerations when choosing recombinant proteins for your research applications.
Non visible textResearch Area
New ways to study the elusive exosome
Written by Poppy Nathan, Proteintech Field Marketing Representative and PhD Student at the University of Birmingham
Non visible textApplication Note
GFP (green fluorescent protein): Properties, origin, specifications, tips
Everything you need to know about GFP: properties and applications
Non visible textApplication note
Ask a Flow Cytometry Expert: Your Questions Answered
Dr. Gareth Howell, Senior Experimental Officer and Flow Core Facility Manager at the University of Manchester answers your questions.
Non visible textApplication Note
Tips and tricks for immunoprecipitation of low abundant proteins
For a successful IP, the abundance or expression level of the POI is crucial. If a POI is strongly expressed and highly abundant, then the IP will most likely be straightforward, with no difficulties.
Non visible textLab Life
Navigating a PhD in a Pandemic
Tips and advice for navigating a PhD during a global pandemic, written by guest blogger Francesca Lewns.
Non visible textFeatured Products
An alternative Nanobody format that combines its special epitope binding properties with the detection options for Fc-domains
A new Swiss army knife for immunofluorescence: Chimeric heavy chain antibodies
Non visible textApplication Note
To dye or not to dye? Understanding the different viability dyes available for flow cytometry
Learn about the types of viability dyes available to help you achieve reliable and accurate results in flow cytometry.
Non visible textApplication Note
Seizing the day: How to save time on your immunoassay experiments
When time in the lab is limited, learn how to save precious hours on your IP, IF, and western blotting experiments.
Non visible textApplication Note
Conjugation of fluorescent dyes to Nanobodies
The conjugation of the binding domains of camelid heavy chain antibodies, also known as VHHs or Nanobodies, to fluorescent dyes is generating versatile tools for fluorescence microscopy applications like immunofluorescence (IF) and super resolution microscopy (SRM):
Non visible textResearch Area
Neuropilin-1 joins ACE2 as SARS-CoV-2 gateway
Landmark paper in Science detailing how Neuropilin-1 is required for SARS-CoV-2 host cell entry, using Proteintech and Chromotek antibodies.
Non visible textApplication Note
Intracellular Flow Cytometry Staining Protocol
Understand the different approaches to intracellular staining for flow cytometry in this "do-it-yourself" guide to fix and perm.
Non visible textCareer Development
Interview with an early-career scientist: Dr Chaitali Mukherjee
Hear from an early-career scientist about her first-author publication in Cell Metabolism and her academic journey
Non visible textResearch Area
Using Flow Cytometry with Epigenetics
Experimental considerations for using flow cytometry to investigate epigenetics.
Non visible textResearch Area
Exosomes: A big role for a small player
Exosomes uncovered. Recent research and the role of exosomes in disease
Non visible textApplication Note
V5-Tag: Properties
V5-Tag: Properties, origin, specifications, antibodies & Nanobodies
Non visible textApplication Note
Which beads should I use for my immunoprecipitation?
In detail, all beads have very low background binding due to the highly specific binding of the Nano-Traps.
Non visible textResearch Area
What do you get when you buy a GMP-grade product?
GMP Manufacturing, benefits, and ISO certifications explained
Non visible textApplication Note
Comparing BCA, Bradford, and UV protein quantification methods for scientists
Understanding the pros and cons of BCA, Bradford, and UV methods of protein quantification
Non visible textCareer Development
Interview with an early-career scientist: Dr Nadav Weinstock
Hear from the lead author of a landmark paper in Neuron about his research into Krabbe Disease
Non visible textApplication Note
How to reduce autofluorescence
Understand the causes behind autofluorescence and how to deal with this problem.
Non visible textResearch Area
Characterization of COVID-19 antibodies by Bio-Layer Interferometry using Nano-CaptureLigands
Binding of the immobilized COVID-19 antibodies to their target, the receptor-binding domain (RBD) of the SARS-CoV2 Spike protein, is characterized with regards to ka, kd, and KD.
Non visible textApplication Note
The advantages of CoraLite direct staining for IF
Video highlights of how CoraLite® antibodies can streamline your multiplex immunofluorescence staining.
Non visible textResearch Area
Serious about Serology: Understanding COVID19 Antibody Tests
Serious about serology: Understanding COVID19 Antibody Tests
Non visible textResearch Area
How fluorescent proteins can be applied in SARS-CoV-2 research
GFP or RFP and their derivatives are commonly used for virus research
Non visible textResearch Area
Cellular senescence and markers
How do cells come to be under "cellular arrest"? Learn what cell senescence is and how it is relevant in disease.
Non visible textApplication Note
SNAP-tag and CLIP-tag overview
SNAP-tag and CLIP-tag are self-labeling protein tags. The SNAP-tag protein and the CLIP-tag protein can be fused to a protein of interest (POI) and used for cellular and biochemical analysis.
Non visible textApplication Note
Halotag
HaloTag is a protein tag. The HaloTag protein can be fused to your protein of interest (POI) which enables it for cellular and biochemical analysis. It can also be used for live cell imaging.
Non visible textResearch Area
Neutralizing antibodies and their role in COVID-19 research
Weathering the Cytokine Storm
Non visible textApplication Note
Do you purify a special protein?
Why Spot-Tag can be used to purify any protein
Non visible textApplication Note
Spot-Trap or Spot-Cap?
Our Sales Rep answers one of the most frequent customer questions
Non visible textResearch Area
Green fluorescent protein (GFP) in plant research
Why does GFP work so well in plants?
Non visible textResearch Area
Virus research using GFP
References from the last 4 years in virus research using GFP and ChromoTek's GFP-Trap
Non visible textApplication Note
Antibody Specificity and affinity in Immunoprecipitation
What is antibody specificity and affinity? And why is it important for immunoprecipitation?
Non visible textApplication Note
Immunoprecipitation without additional bands
Pull-down of proteins with Nano-Traps
Non visible textApplication Note
GFP and RFP-Booster for better immunofluorescence imaging
ChromoTek's GFP Nanobodies and RFP Nanobodies conjugated to fluorescent dyes
Non visible textApplication Note
The importance of blocking when using Nano-Secondary reagents for IF
Blocking improves the sensitivity by reducing nonspecific background and therefore increases microscopy image quality. Insufficient blocking results in higher background noise.
Non visible textApplication Note
How to overcome the limitations of antibody-based affinity resins for protein purification
Spot-Cap for highly effective one-step protein purification
Non visible textCareer Development
How fellowships can advance your scientific career
Thoughts and advice to anyone thinking of applying for fellowships worldwide
Non visible textApplication Note
Tags for protein purification
Overview over commonly used affinity tags in protein purification
Non visible textApplication Note
Split fluorescent protein technology
The ChromoTek GFP-Trap has been successfully applied to multiple assays using different split GFP variants
Non visible textResearch Area
How to solve the genome DNA damage
DNA damage repair mechanisms: in the prevention of age-related diseases and tumorigenesis
Non visible textApplication Note
The best anti-GFP antibody for immunoprecipitation: GFP-Trap
Comparison of GFP-Trap and anti-GFP IgG antibody
Non visible textLab Life
13 tips how to be more eco-friendly in the lab
Eco-friendly lab - Why Bother?
Non visible textApplication Note
Spot Capture and Detection Peptide Tag and Nanobody
The Spot Nanobody for multiple capture & detection applications of Spot-tagged proteins
Non visible textApplication Note
Dying to see what’s inside: Staining organelles
Visualizing the interior of organelles helps us to understand their molecular mechanisms and study cell abnormalities.
Non visible textApplication Note
Proteases for Western Blotting: Choosing the right tools for the job
How proteases can help you to better understand your Western blot results
Non visible textApplication Note
What is one-step immunostaining?
This method reduces incubation time, hands-on time and the number of washing steps.
Non visible textApplication Note
Tips and tricks for successful Mass spec experiments
Troubleshooting guide to address the most common obstacles during mass spectrometry experiments.
Non visible textApplication Note
Important facts to know when using Nano-Secondary reagents
What I should know when I'm working with Secondary antibodies or Nano-Secondary reagents
Non visible textResearch Area
What is the difference between necrosis and apoptosis?
Apoptosis and necrosis are two distinct forms of cell death with different causes, mechanisms, and consequences. Understanding their differences is crucial for interpreting experimental results in cell biology, pathology, and drug development research.
Non visible textApplication Note
Fluorescent protein tags
Types of fluorescent protein tags and comparisons
Non visible textResearch Area
Missing pieces: DNA damage
Various environmental and endogenous factors cause DNA damage, resulting in cell degeneration, aging, or tumorigenesis.
Non visible textApplication Note
IF imaging: Widefield versus confocal microscopy
An overview of microscopy techniques and advice on when to use each method.
Non visible textApplication Note
Chromatin Immunoprecipitation (ChIP) with RFP-tagged or mCherry-tagged proteins
RFP-Trap for ChIP analysis in order to detect enrichment of mCherry-tagged proteins at DNA binding sites
Non visible textApplication Note
Mass spec essentials
An overview of mass spec sample preparation, sample purification and data analysis.
Non visible textCareer development
The state of the international scientist in the U.S.
Supporting foreign-born scientists contributing to biomedical research in the U.S.
Non visible textCareer Development
Why studying abroad makes sense: Scientists share their story
Perspectives on Emigrating for Academic Career Advancement
Non visible textResearch Area
CRISPR-Cas9, TALENs and ZFNs - the battle in gene editing
Technological advances are crucial for innovative biological research. ZFNs, TALENs, and CRISPR/CRISPR-associated Cas have revolutionized genome editing.
Non visible textResearch Area
Quantitative MiRNA Profiling: How To Choose The Best RNA Sequencing Method?
RNA sequencing (RNA-seq) is a widely used method employing next generation sequencing (NGS) to discover and quantify the abundance of RNA molecules in biological samples.
Non visible textResearch Area
Are Mesenchymal Stem Cells (MSCs) true stem cells?
A guide to MSC characteristics, differentiation pathways and supporting reagents for MSC culture.
Non visible textCareer Development
7 secrets to a successful scientific presentation
Fight the fear of presenting your scientific research.
Non visible textResearch Area
A closer look at Embryonic Stem Cells (ESCs) – biological concepts
An overview of the history, features and applications of ESCs.
Non visible textApplication Note
TurboGFP: Properties, Sequence, MW, origin
Everything you need to know about TurboGFP: properties and applications
Non visible textLab Life
Life Scientist Appreciation Week University of Chicago
June 17-21 2019. #CelebrateLifeScientists #LSAW
Non visible textResearch Area
Why Human Cell Expressed Proteins Are Superior for Clinical Applications
Learn about the advantages of using human cell expressed recombinant proteins in your clinical applications.
Non visible textResearch Area
Cell fate commitment and the Waddington landscape model
Waddington’s epigenetic landscape has been used for decades to illustrate the progressive restriction of cell differentiation during development.
Non visible textApplication Note
How do I know if the antibody will cross-react?
Antibody cross-reactivity can compromise your results, but it doesn't have to. This guide explains what causes it, how to predict it with sequence homology, and practical solutions to ensure specific binding in your Western blot, IP, and staining experiments.
Non visible textApplication Note
5 Tips for immunostaining using multiple labels
Our advice on microscope set-up, fluorophore choice and controls to improve your multi-label IF.
Non visible textResearch Area
How does blood coagulate?
Almost 1/10th of a human's body weight is made up of blood (male ±5.5 liters; female ±4.5 liters). Besides transporting oxygen and nutrients to our cells, blood plays a key role in our immune system and temperature homeostasis (1).
Non visible textResearch Area
ICF syndrome: A gene-silencing chromatin disorder
Immunodeficiency, Centromere region instability, Facial anomalies syndrome (ICF) is a rare autosomal recessive disease that damages the immune system.
Non visible textResearch Area
Induced pluripotent stem cells (iPSCs) and modeling neurodegenerative disease in the dish
iPSCs and their potential for resetting the biological clock.
Non visible textApplication Note
Polyclonal vs. monoclonal antibodies
Both types have their unique advantages and disadvantages and can be used in a wide variety of applications.
Non visible textResearch Area
Epigenetic implications in brain functions - Neuroepigenetics
Environmental factors, stress, learning or drug exposure lead to active regulation of DNA structure in the nervous system.
Non visible textLab Life
Proteintech supports young scientist to attend EMBL conference
Proteintech supports young scientists – Non-Coding Genome EMBL conference Heidelberg, Germany, 13–16 Sep 2017
Non visible textResearch Area
Actin Chromobody: Visualization of actin dynamics – NOT alteration
The ChromoTek Actin Chromobody is the only probe that does not interfere with actin filament dynamics.
Non visible textResearch Area
Rett syndrome - the second leading cause of mental retardation in women
An overview of Rett syndrome: clinical features, neuropathology and animal models.
Non visible textCareer Development
How to Write a Good Scientific Abstract
Our top tips and how to write an effective scientific abstract.
Non visible textCareer Development
How to be a great lab manager: 6 tips
Our advice to help you be a great lab manager and manage your time better.
Non visible textApplication Note
Protein or peptide antigen? Advantages and disadvantages
Peptide versus whole protein immunogens for antibody production
Non visible textResearch Area
Immunotherapy: boosting the immune system to fight rare cancers
Immunotherapy: how does it work and what do we know so far?
Non visible textCareer Development
Top Tips for a Successful Transition to a Postdoc Role
Advice and tips given by postdocs from all over the world.
Non visible textResearch Area
The Complexity of Proteins
How do proteins achieve so much functional diversity from only 20 amino acid building blocks?
Non visible textResearch area
WNT Pathway - Defining the Canon
How did scientists figure out such a complicated pathway?
Non visible textResearch Area
Type 2 Diabetes Pathogenesis
Does Insulin Resistance Cause hyperinsulinemia?
Non visible textResearch Area
Metabolic Regulation of Cell Death
A matter of life and (cell) death...
Non visible textResearch Area
Epigenetics Modifications - DNA methylation
DNA Methylation: An Overview
Non visible textPoster
Alzheimer's Disease Signaling Pathway
Download or request a pathway poster for your lab
Non visible textPoster
MAPK Pathway: New Poster Available
Download or request a pathway poster for your lab
Non visible textResearch Area
Epigenetics - DNA layers and chromatin modeling
Introduction to Epigenetics
Non visible textApplication Note
mNeonGreen vs GFP
mNeonGreen: A green fluorescent protein that is not a GFP variant
Non visible textResearch Area
Post Translational Modifications: An Overview
Nature’s escape from genetic imprisonment
Non visible textFeatured Products
New anti-mNeonGreen antibody & Nano-Trap for Immunofluorescence & Immunoprecipitation
Novel tools for the analysis of mNeonGreen fusion proteins
Non visible textApplication Note
Affinity purification, depletion, and immunoprecipitation of GST-tagged proteins
Publications using ChromoTek's GST-Trap in various applications
Non visible textPoster
Pathway Poster Library
View our interactive signaling pathways or requested a printed poster to decorate your lab
Non visible textPoster
Caspase Cascade Pathway: New Poster Available
Download a pathway poster for your lab
Non visible textLab Life
The Incubator Podcast Highlights
Season 2 is here! Here's a re-cap of the topics discussed in Season 1
Non visible textResearch Area
Cancer stem cells as a key to cure cancer
A guide to cancer stem cells and the markers that can be used to isolate cell populations.
Non visible textApplication Note
How To Optimize Your ELISA Experiments
Technical tips for better ELISA results
Non visible textApplication Note
Immunoprecipitation of Myc-tagged proteins – How it works
ChromoTek Myc-Trap® is an optimized tool for immunoprecipitation (IP/Co-IP)
Non visible textResearch Area
Autophagy in the Pathogenesis of Disease
Aging, cancer, inflammation, infection, the microenvironment, and neurodegenerative disease
Non visible textApplication Note
9 Tips to optimize your IF experiments
Want to improve your IF experiments? Our 9 tech tips will help you achieve successful Immunofluorescence staining
Non visible textFeatured Products
The most frequently cited monoclonal anti-RFP antibody
Immunofluorescence and immunohistochemistry with the ChromoTek's anti-RFP antibody [5F8]
Non visible textResearch Area
Atrial and ventricular myocytes
A guide to atrial and ventricular myocyte markers
Non visible textApplication Note
A beginners guide to Immunohistochemistry
12 steps to get you started with immunohistochemistry
Non visible textApplication Note
Lysate Preparation: How Do I Optimize My Extraction?
Technical tips to improve your sample preparation for Western blot
Non visible textApplication Note
Lysate Preparation: Why is RIPA Buffer Best for Western Blot
What is in a lysis buffer? Learn the purpose of each component.
Non visible textResearch Area
Visualize the onset of metastasis with Chromobodies
The potential of the chromobody technology to study EMT in cell-based screening systems is reviewed by Maier et al., Scientific Reports 2015.
Non visible textResearch Area
Mitochondria: More than just the powerhouse of the cell
An introduction to mitochondria in health and disease.
Non visible textApplication Note
8 Top Tips For Immunoprecipitation
8 tips on how to perform better IP experiments.
Non visible textResearch area
Hidden Secrets Of The Heart
The plot thickens… and so does the artery wall!
Non visible textResearch Area
Mitochondria: Center of Cellular Signaling and Energetic Balance
Mitochondrial markers
Non visible textLab Life
Career Development Podcast: The Incubator
Fret over your scientific career no longer. Proteintech is sponsoring a monthly career development podcast, The Incubator.
Non visible textApplication Note
10 tips on how to best optimize siRNA transfection
Improve your siRNA transfections with these expert tips.
Non visible textResearch Area
The Neurochemistry of Parkinson's Disease
An overview of brain neurochemistry in Parkinson's Disease, written by guest blogger Ashley Juavinett.
Non visible textResearch area
Molecular markers for liver cancer
Discover molecular markers for hepatocellular carcinoma.
Non visible textApplication Note
Nano-Booster FAQs
Frequently asked technical questions on GFP-and RFP-Booster
Non visible textApplication Note
How to elute GFP-fusion protein from the GFP-Trap®
Tips and tricks
Non visible textApplication Note
Loading Control Antibodies for Western Blotting
Discover the most appropriate loading controls for your Western blot experiments.
Non visible textResearch area
Nano Diamonds and Cancer
An introduction to nanoparticle delivery in cancer, written by guest blogger Veronika Benson.
Non visible textResearch Area
Fast Phospho-rward: Making Phosphoprotein Antibodies
A historical perspective on the production of phospo-antibodies.
Non visible textApplication Note
Immunofluorescence: Tips for Immunostaining Cultured Cells
Improve your IF with these expert tips from Proteintech scientists.
Non visible textBlog
Recalling when and where: How the brain processes episodic memory
Introduction
Non visible textApplication Note
8 Top Tips for Subcellular Fractionation
8 technical tips for successful subcellular fractionation
Non visible textResearch Area
Antibodies for Cilia Development
A guide to key targets in cilia development and ciliogenesis.
Non visible textApplication Note
Tech Tips | In search of low molecular weight proteins
Learn how to improve your Western blot for low molecular weight proteins.
Non visible textResearch area
Can a Bacterial 'Brain' Control a Robot?
A perspective on how technology can be used to control biological systems, by guest blogger Ashley Juavinett
Non visible textBlog
Making the connection between brain and body
We’re connected, from head to toe...
Non visible textBlog
GSK3 pathway poster
Request a printed copy of the GSK signaling pathway for your lab.
Non visible textResearch Area
New nuclear Actin-Chromobody® plasmid
It is our pleasure to announce the availability of a new member of our Actin Chromobody® plasmid family:
Non visible textBlog
Can man's best friend help cure cancer
Humans are not the only species on earth that develop cancer; our beloved pets can also succumb to the ‘Big C’ too.
Non visible textApplication Note
Seeing Clearly: Tips For Staining Cleared Tissue With Antibodies
Improve your immunofluorescence in cleared tissue with expert tips from our guest blogger, Ashley Juavinett.
Non visible textBlog
I Just Want Your Extra Time And Your...KISS-1
Learn about the function of the KISS-1 protein, written by guest blogger Sophie Quick.
Non visible textResearch Area
Breast Cancer | 7 Interesting Facts
Learn something you didn't know about breast cancer.
Non visible textResearch Area
MHC Class I: The Cancer Connection
The relationship between the major histocompatibility complex (MHC) and cancer.
Non visible textApplication Note
7 Tips For Optimizing Your Western Blotting Experiments
How to optimize your western blot protocol
Non visible textBlog
Special offer for newsletter sign up
Sign up to the Proteintech regular eNewsletter to receive a free lab survival kit.
Non visible textApplication Note
Antibody Comparison Sites: How to Find Your Perfect Antibody Faster
A guide to finding the best antibody for your application
Non visible textCareer Development
Example PhD interview questions and answers
How to succeed in your PhD interview with answers to frequently asked questions
Non visible textResearch area
The multi-organ impact of COVID-19
Written by Alicia Haydo M.Sc., a PhD candidate at Goethe University Frankfurt am Main
Non visible textResearch area
COVID-19 Neuropathology Similar to Alzheimer’s Disease – The Role of the Ryanodine Receptor in “Long COVID”
Written by Leah Sittenfeld, M.D. Candidate at New York Medical College
Non visible textResearch area
Second Cancer After Breast Cancer
Explore why breast cancer survivors are more likely to develop a second cancer after the first one has been cured.
Non visible textResearch area
Primary Cilia and Regulation of Blood Pressure (“The Silent Killer”)
Discovering cilia’s role as a regulator of arterial blood vessels and its involvement in disease.
Non visible textApplication note
Imaging the Rainbow: Multiplexing with Same-Species Antibodies for Immunofluorescence
Improve your IF multiplexing with these expert tips from our guest blogger, Derek Sung (IG: @Immunofluoresence)
Non visible textCareer Development
How to succeed in an interview – Academia vs. Industry
Find out how to best prepare for an interview with top tips from our experts with successful academic and industry careers.
Non visible textCareer Development
How to write an industry resume/CV
Are you a life-scientist looking to change your career path? Learn how to write an industry resume/CV
Non visible textCareer Development
Ask the Experts: Transitioning from Academia to Industry
Read some of the engaging Q&A session from our recent careers workshop on moving from an academic to industry position.
Non visible textPoster
JAK/STAT Signaling: New Pathway Poster
Download a pathway poster for your lab
Non visible text
Able®