Nano-Secondaries

High resolution and low background

Nano-Secondaries® are the next level of secondary antibodies providing higher resolution, lower background, and cleaner images. Nano-Secondaries are Nanobodies/ VHHs conjugated to Alexa Fluor® fluorescent dyes and bind to primary antibodies with high affinity in a species and subtype specific manner:

  • Faster staining: One-step incubation
  • Higher resolution
  • Low background
  • No cross-reactivity and high subclass specificity

Nano-Secondaries can be applied in:

Explore Nano-Secondaries
Staining image of secondary nanobody ChromoTek Nano-Secondary® alpaca anti-mouse IgG1, recombinant VHH, Alexa Fluor® 488. The anti-mouse IgG1 Nano-Secondary is subclass-specific and does not cross-react with IgGs from other commonly used species (here rabbit) and with mouse IgG2b and IgG3 subclasses.