Find out everything you need to know about our products here.
Our antibodies are under guarantee for 1 year after purchasing. However, internal studies show that the antibody will remain functional for a minimum of five years when properly stored.
Please store the antibody in the original tube at -20°C and do not aliquot. Our antibodies are stored in 50% glycerol which prevents them from freezing.
You can find the detailed information for each antibody on their product datasheet. Please search and click the antibody of your interest to be directed to the datasheet for your antibody which includes validated applications, images, etc. If you would like to print the datasheet, click the print icon below the Catalog Number.
The concentration of a given antibody is found on the individual datasheet.
On the product page of your antibody, please click the link beside ‘Immunogen’ to view a description of the immunogen. Should you require more detailed information, please speak to a member of staff.
We use full-length recombinant proteins as immunogens to generate our antibodies that can be used as blocking reagents. However, we do not test the neutralizing effect of the antigen (the recombinant protein) and do not have any other blocking peptides against our antibodies.
We test our antibodies on human and mouse cells or tissues. To find information on other species, please contact us.
This depends on the extent of protein sequence similarity between the immunogen and the potential cross-reactive protein sequence. A pair-wise sequence alignment can be performed online through NCBI-BLAST website (Link to http://blast.ncbi.nlm.nih.gov/Blast.cgi).
Each product pages shows the number of publications and has links to the publication, if the antibody has already been cited.
Several reasons can cause a difference in band size, such as multimers, gel migration, relative charges, splice variants, post-translational modification or cleavage. For more information, please contact our tech support.
Each monoclonal antibody has a clone number that is specific for its single clone of hybridoma cells. The clone number can be found on the product page next to the catalog number.
We usually add 6x His tags or GST tags to the N-terminus of proteins and these proteins are then purified by Ni2+ and glutathione affinity chromatography respectively.
The His-tagged recombinant protein is usually stored in 1x PBS buffer (58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH 7.4) containing 300mM imidazole.
The GST-tagged recombinant protein is usually stored in 1x PBST buffer (58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, 1% Triton-X100, pH 7.4) containing 100mM glutathione (GSH).
Antibody-coated 96-well microplate, assay standard, detection antibody, HRP-conjugated antibody, sample diluent, detection diluent, wash buffer, tetramethylbenzidine (TMB) substrate, stop solution, and plate cover seals.
All Proteintech ELISA kits are sandwich ELISAs.
Yes, all plates are pre-coated with an antibody that is specific for the target.
It is recommended to run the experiment in duplicate at a minimum.
No, different lot numbers cannot be mixed.
Incubation times should be followed exactly as stated in the manual.
Please follow the instructions in the manual.
Most of our ELISA kits are validated for cell culture supernatant, plasma, or serum. However, the Proteintech Guarantee covers all sample types.
The ELISA sensitivity is highlighted in the data sheet for each kit.
There are multiple explanations: incorrect storage, incorrect incubation time, improper antibody, insufficient washing, etc. For more information, please contact technical support.
Improper washing, contaminated reagents, samples, or wells. For more information, please contact technical support.
Custom production projects can typically take up to 6 months to complete, but due to the complexity of the process, we cannot always give a guarantee of an exact schedule. Please see below for the schedule of our 102-day immunization protocol, which demonstrates our commitment to making sure that you receive an antibody that works.
102-Day Immunization Protocol
|Day 0 Pre-Immune Bleed||Day 56 Test Bleed & ELISA||Day 88 Production Bleed|
|Day 1 Primary Injection||Day 60 Boost 3||Day 102 Final Bleed|
|Day 28 Boost 1||Day 74 Production Bleed|
|Day 42 Boost 2||Day 78 Boost 4|
No, unfortunately we do not currently accept fusion proteins expressed by our customers.
KLH is the default carrier protein. This is linked to the peptide via a terminal cysteine (added by Proteintech®). If you have cysteine residue in the middle of your peptide then we can use MAP beads instead.
Each antigen will be injected into two rabbits.
In our experience, full-length fusion protein antigens usually generate antibodies with higher titers and higher sensitivities. We recommend the full-length fusion protein procedure if your protein of interest has low sequence similarity with other proteins. However, if you need an antibody that is specific to a certain protein, the sequence of which is highly similar to other proteins, we strongly recommend using a peptide antigen. Other reasons you might choose a peptide antigen include:
1. Not having the cDNA for the protein of interest;
2. Expression of the full-length fusion protein has not been successful for some reason.
No, we carry out custom production of antibodies for proteins from any species, depending on antigenicity.
Each rabbit will yield approximately 12 ml of serum in each production bleed and 45–50 ml of serum from 90–100 ml of blood in the final bleed, totalling about 150 ml of antiserum from two rabbits.
We decide that based on the ELISA results with the test bleeds. If both rabbits give good results, we will purify 15 ml of serum from each rabbit. If one rabbit gives better results than the other, we will purify 30 ml of serum from this rabbit.
It depends on the concentration of the specific antibody in the serum provided. Usually we can get 2–10 ml of purified antibody from 15 ml of antiserum.
Please visit the custom production section for more information on the service we provide.