Uni-rAb™
Recombinant Monoclonal Antibodies
Uni-rAb™ antibodies are the latest generation of recombinant monoclonal antibodies developed using Proteintech’s Antigen-Specific B-Cell Cloning & Engineering (ABCE™) platform. The ABCE™ platform achieves stable and high expression of recombinant monoclonal antibodies by cloning antibody heavy and light chain sequences derived from antigen-specific B cells into high-yield expression vectors followed by their introduction into suitable mammalian expression systems.
Uni-rAb™ antibodies are the latest generation of recombinant monoclonal antibodies developed using Proteintech’s Antigen-Specific B-Cell Cloning & Engineering (ABCE™) platform. The ABCE™ platform achieves stable and high expression of recombinant monoclonal antibodies by cloning antibody heavy and light chain sequences derived from antigen-specific B cells into high-yield expression vectors followed by their introduction into suitable mammalian expression systems.
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Monoclonal antibodies with native VH-VL pairings
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Unparalleled lot-to-lot consistency
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Excellent specificity & high affinity
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High-throughput production
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High purity
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Available in carrier-free formulations
ABCE™ Platform
Leveraging more than 20 years of antibody development experience, Proteintech’s Antigen Specific B-Cell Cloning & Engineering (ABCE™) platform combines the in-depth technical expertise of our R&D team with recent advancements in antibody technology and cutting-edge automation to ensure rapid and high-throughout production of a variety of off-the-shelf as well as custom recombinant antibodies.
Uni-rAb™ recombinant antibody development workflow on the ABCE™ platform.
Advantages
Precise antigen design
Generating precise and high-quality antigens plays a critical role in the successful development of antibodies. As a world-renowned manufacturer of antibodies, Proteintech has developed a vast portfolio of over 15,000 rabbit polyclonal and mouse monoclonal antibodies covering almost 2/3 of the human proteome. Antigens that were used for the development of these antibodies have been already verified and therefore, can serve as valuable raw materials for the rapid generation of Uni-rAb™ recombinant antibodies. Moreover, our scientists bring decades of experience to help you generate antigens tailored to the specific needs of your project, including antigens for post-translational modifications such as phosphorylation, acetylation, etc. or challenging targets such as membrane proteins.
Antigen-specific single B cell sorting
Antigen-specific single B cell sorting allows the isolation of functional monoclonal antibodies reactive against antigens in their native conformation that predominantly occur in vivo and are difficult to reproduce in vitro. This ultimately leads to the generation of Uni-rAb™ recombinant antibodies that have native VH-VL pairings which is critical for maintaining antibody specificity and functionality but is often lost in other antibody development approaches such as phage display. The ABCE™ platform utilizes antigen dual-labeling and multiparametric FACS to exclude low-affinity and non-specific B cells, thereby facilitating the isolation of antigen-specific B cells with high efficiency.
Phospho-Histone H2A.X (Ser139) Recombinant antibody
Antigen-specific single B cells sorting results in a high percentage of positive clones as verified by ELISA.
Rigorously screened and validated
Uni-rAb™ recombinant antibodies are rigorously screened and validated to ensure the highest level of performance across multiple applications. All Uni-rAb™ antibodies undergo screening at three different stages during their development, including: 1) high-throughput initial supernatant screening, 2) high-throughput re-validation of purified antibody, and 3) final functional validation prior to the formal release of the antibody. Screenings are conducted using various immunoassays, including Western blot (WB), immunofluorescence (IF), and flow cytometry (FC) and the screening data obtained at the three stages are carefully analyzed to confirm correlation.
Moreover, Uni-rAb™ recombinant antibodies are subjected to a label-free biomolecular interaction analyzer which uses Biomembrane interferometry (BLI) technology to detect the affinity index between antibodies and antigens. Majority of Uni-rAb™ recombinant antibodies have been validated to have dissociation constants <10-10.
Uni-rAb™ antibodies are screened at three different stages during their development on the ABCE™ platform.
98005-1-RR (Mouse IL-17A)
83188-2-RR (Human MMP13)
Measurement of Uni-rAb™ antibody affinity using biolayer interferometry (BLI).
Two-step purification
Uni-rAb™ recombinant antibodies are purified via a two-step purification process, which includes protein A purification followed by mixed-mode cation/anion exchange chromatography. This helps reduce the impact of residual impurities such as nucleases, host proteins, shed Protein A, antibody aggregates and invalid antibody fragments on antibody quality, ensuring high purity and consistency between different batches of Uni-rAb™ antibodies.
Two-step purification of Uni-rAb™ recombinant antibodies.
Unparalleled lot-to-lot consistency
Since the development of Uni-rAb™ recombinant monoclonal antibodies relies on the in vitro cloning and expression of defined heavy and light chain sequences derived from antigen-specific B cells, the entire production process can be standardized and replicated whenever needed once the antibody sequences are known. Being biologically defined eliminates the risks of genetic drift and instability as seen in traditional hybridoma-derived monoclonal antibodies, thereby ensuring unparalleled lot-to-lot consistency and reproducibility of experimental data.
Western blot analysis of various cell lysates using three separate batches of Uni-rAb™ Beta Actin Recombinant Antibody (81115-1-RR).
High-throughput production
Single B cell-based antibody development offers a highly efficient platform for the rapid generation of recombinant antibodies with excellent specificity against any antigen of choice. Higher number of positive clones with natural VH-VL pairings can be obtained from a small number of cells and in a much shorter time compared to traditional hybridoma-based or phage display approaches. For targets with pre-qualified antigens from our rabbit polyclonal and mouse monoclonal antibody development pipelines, lead candidates for recombinant Uni-rAb™ antibody production can be identified in as fast as 4 weeks.
State-of-the art instrumentation utilized on the ABCE™ platform.
Validation Data
Various lysates were subjected to SDS PAGE followed by western blot with Uni-rAb™ AKT1 Recombinant Antibody (80816-1-RR).
Wild-type and ATG5 knockout Hela cells were subjected to SDS PAGE followed by western blot with Uni-rAb™ ATG5 Recombinant Antibody (81803-1-RR).
Untreated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with Uni-rAb™ Phospho-AKT (Ser473) Recombinant Antibody (80455-1-RR). The membrane was stripped and re-blotted with GAPDH Antibody as loading control.
Untreated and Trichostatin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with Uni-rAb™ Acetyl-Histone H2B (Lys5) Recombinant Antibody (83171-4-RR). The membrane was stripped and re-blotted with Beta Actin Antibody as loading control.
IF analysis of (4% PFA) fixed SH-SY5Y cells using Uni-rAb™ TDP-43 Recombinant Antibody (80002-1-RR, green) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG (H+L). Cells were co-stained with CoraLite555-Phalloidin (red).
IF analysis of (4% PFA) fixed mouse heart tissue using Uni-rAb™ CoraLite® Plus 488 Smooth Muscle Actin-specific Recombinant Antibody (CL488-80008). Nucei were stained with DAPI.
IF analysis of (4% PFA) fixed HeLa cells using Uni-rAb™ GP73/GOLPH2 Recombinant Antibody (81893-1-RR, green) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CL594-Phalloidin (red).
IF analysis of (-20°C Methanol) fixed untreated and Chloroquine treated HeLa cells using Uni-rAb™ LC3 Recombinant Antibody (81004-1-RR, green), CoraLite®488-Conjugated Goat Anti-Rabbit IgG (H+L) and CoraLite®594-Conjugated Beta Actin Monoclonal Antibody (CL594-66009).
IHC analysis of FFPE human pancreas cancer tissue using Uni-rAb™ NRF2, NFE2L2 Recombinant Antibody (80593-1-RR). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC analysis of FFPE human colon tissue using Uni-rAb™ a-SMA-specific Recombinant Antibody (80008-1-RR). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC analysis of FFPE human breast cancer tissue using Uni-rAb™ Vimentin Recombinant Antibody (80232-1-RR). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC analysis of FFPE rat dorsal root ganglion tissue slide using Uni-rAb™ Piezo1 (extracellular domain) Recombinant Antibody (82625-4-RR). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
1x10^6 human PBMCs were surface stained with 0.25 ug of Uni-rAb™ Anti-Human TIGIT Rabbit Recombinant Antibody (98023-2-RR) or Isotype Control, and PE-conjugated Goat Anti-Rabbit IgG (H+L). Cells were then stained with APC Anti-Human CD45RO. Cells were not fixed. Lymphocytes were gated.
1x10^6 human PBMCs were surface stained with 0.25 ug of Uni-rAb™ Anti-Human CD28 Rabbit Recombinant Antibody (98032-1-RR) or Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cell were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.
1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug of Uni-rAb™ Anti-Human CEACAM8/CD66b Rabbit Recombinant Antibody (98012-1-RR) or Isotype Control, and PE-conjugated Goat Anti-Rabbit IgG (H+L). Cells were not fixed.
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human Fas/CD95 Rabbit Recombinant Antibody (98034-1-RR) or Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG (H+L). Cells were then stained with APC Anti-Human CD45RO (UCHL1) (APC-65150, Clone: UCHL1). Cells were not fixed.
Uni-rAb™ Categories
Unconjugated Uni-rAb™ Antibodies
Wide collection of unconjugated monoclonal recombinant antibodies against key targets covering multiple areas of research and validated in multiple applications.
Conjugated Uni-rAb™ Antibodies
Monoclonal recombinant antibodies conjugated to Proteintech’s proprietary range of CoraLite Plus dyes, perfect for IF and FC applications.
PBS-Only Uni-rAb™ Antibodies
Monoclonal recombinant antibodies free of carriers including BSA, sodium azide and glycerol offering the flexibility for use in various downstream applications including conjugation and mass cytometry.
Uni-rAb™ Antibodies for Flow Cytometry
Monoclonal recombinant antibodies against popular CD markers, cytokines and intracellular targets optimized for flow cytometry.
Uni-rAb™ Matched Antibody Pairs
Matched recombinant antibody pairs consisting of a capture antibody and a detection antibody specific for different epitopes on the same antigen.
All Uni-rAb™ Antibodies
Explore our rapidly expanding portfolio of all monoclonal recombinant antibodies with hundreds of new targets being added every month.
Custom Services
If you are interested in custom antibody development services, look no further. Proteintech’s scientists bring over 20 years of experience in antigen design, expression and purification to help you with your custom antibody development project. Our ABCE™ platform offers the flexibility of multi-species antibody discovery for desired targets with a rapid turnaround
Projected timeline for custom antibody development on the ABCE™ platform.
In addition to full-length antibodies, we can develop a variety of custom antibodies tailored to specific project needs, including scFv antibodies, Fc fusion antibodies, Fc mute and other Fc modifications, chimeric antibodies, bispecific antibodies and antibody fragments such as Fab and F(ab’)2.
Types of custom antibodies that can be developed by Proteintech.
Proteintech can help you with a wide range of custom antibody services as listed below.
Single B Cell Services Available for
- Rabbit
- Mouse
- Rat
- Alpaca
- Sheep
- Chicken
Antibody Engineering Services
- Fab, F(ab’) fragments
- scFv, scFv-Fc, VHH-Fc
- Fc mute & other Fc modifications including Fc silencing
- Chimeric antibodies
- Bi-specific antibodies
- PTM-specific antibodies
- Antibody humanization
- Antibody conjugation including site-specific and stoichiometric conjugation
- Antibody-drug conjugates
Antibody Characterization Services
- ELISA and antibody pairing
- FACS binding and blocking
- Functional assays including WB, IP, IHC, IF & FC
- Neutralization test
- Affinity measurement
- EC50/IC50 measurement
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