Nanobodies are the smallest known antibodies (~12-15 kDa vs ~150 kDa of conventional IgG antibodies). They are the antigen binding domains or variable heavy domain of heavy chain antibodies (VHHs) from alpacas, llamas, or camels.
ChromoTek Nanobody-based reagents are thoroughly validated, recombinantly expressed, and ready to use. Due to their size and single chain characteristic, they provide a higher level of performance than conventional IgG antibodies in applications such as immunoprecipitation, immunofluorescence, live cell imaging, biosensor assays, and protein purification.
Chromotek is now part of Proteintech
All ChromoTek Nanobody-based reagents are now available on ptglab.com.
Nano-Traps are ideal for fast, reliable and efficient one-step immunoprecipitation and co-immunoprecipitation. Nano-Traps comprise of Nanobodies conjugated to beads.
Nano-Secondary® reagents use a novel class of secondary antibodies for higher resolution & cleaner images. These reagents consist of Nanobodies conjugated to fluorescent dyes binding to conventional antibodies.
Chromobodies® are fluorescent nanoprobes for real-time and live-cell imaging of intracellular endogenous proteins. Chromobodies are Nanobodies that are genetically fused to a fluorescent protein such as GFP.
ChromoTek's Nano-Boosters & Nano-Labels are very small fluorescent probes optimzed for confocal and super-resolution microscopy. They consist of Nanobodies conjugated to fluorescent dyes.
Nano-Caps® are optimized for effective and cost-efficient protein purification. Nano-Caps consist of Nanobodies conjugated to agarose.
Nano-CaptureLigands are optimized to specifically immobilize human, mouse, and rabbit antibodies to streptavidin/avidin. Nano-CaptureLigands are biotinylated Nanobodies.
Suitable for conjugation to dyes, biotin, beads, surfaces etc. via NHS ester reaction.
ChromoTek's proprietary peptide-tag system for immunoprecipitation, immunofluorescence, and protein purification. Depending on application, Spot Nanobodies are conjugated to agarose, beads, or fluorescent dyes.
Nanobody based reagents show less background, i.e., no heavy and light antibody chains in SDS/PAGE and Western blot.
Due to the small size of Nanobodies, higher image resolution can be achieved.
Live Cell Imaging
Chromobodies® visualize endogenous cellular structures and processes in live cells and real-time.
Fluorescently labeled Nanobodies and antibodies allow for multiplexing.
The small size of Nanobodies enables better tissue penetration and decreases the distance between epitope and label.
Nanobody based tools enable for high reproducibility and low background.
Affinity resins with Nanobodies allow for effective one-step protein purification.
Spot Capture and Detection System
Universal capture and detection system based on Spot-Nanobody for immunofluorescence, immunoprecipitation, protein purification, Western blotting, etc.
On-bead Enzyme Assays
The enzymatic activity can be assessed directly after immunoprecipitation from a cell lysate with Nanobodies.
High Content Analysis
Using Chromobodies, dynamic cellular changes can be visualized and monitored in real time.
Ligand Capture and Affinity Determination
Directed immobilization of antibodies for characterization of biomolecular interactions such as surface plasmon resonance (SPR) or bio-layer interferometry (BLI).
An Enzyme-linked immunosorbent assay (ELISA) is method for the detection of a target molecule in a liquid sample.
An affinity resin for IP of GFP-fusion proteins. It consists of a GFP Nanobody/ VHH coupled to agarose beads.
V5-Trap® Magnetic Agarose
An affinity resin for IP of V5-tagged proteins. It consists of a anti V5 Nanobody/ VHH coupled to magnetic agarose beads
|v5tma||IP, CoIP, ChIP, RIP|
An affinity reagent for IP and purification of Myc-tagged proteins. It consists of a Myc Nanobody/ VHH coupled to agarose beads.
Alpaca anti-mouse IgG1, recombinant VHH, Alexa Fluor® 647
Consists of Nanobodies that bind to mouse IgG1 with high affinity & specificity.
|sms1AF647-1||IF, WB, FC|
Anti-Spot-Tag VHH/ Nanobody conjugated to ATTO594.
Actin-Chromobody® plasmid (TagRFP)
DNA plasmid encoding for anti-Actin VHH (anti-Actin Nanobody) fused to TagRFP.
RFP-Trap® Magnetic Particles M-270
RFP VHH/ Nanobody coupled to Magnetic Particles M-270.
Discovery of Nanobodies, the Alpaca Nanobody advantage and validation of Nanobodies.Non visible text
Spot Capture & Detection System
Universal capture and detection system based on Spot-Tag®, an inert 12 amino acid peptide-tag and Spot-Nanobody for immunofluorescence, immunoprecipitation, protein purification, Western blotting, etc.Non visible text
Request a free sample and test the GFP-Trap & other Nano-Traps yourself.Non visible text
Discovery of Nanobodies, the Alpaca Nanobody advantage and validation of Nanobodies.