Nanobody-based Reagents

Nanobodies are the smallest known antibodies (~12-15 kDa vs ~150 kDa of conventional IgG antibodies). They are the antigen binding domains or variable heavy domain of heavy chain antibodies (VHHs) from alpacas, llamas, or camels.

ChromoTek Nanobody-based reagents are thoroughly validated, recombinantly expressed, and ready to use. Due to their size and single chain characteristic, they provide a higher level of performance than conventional IgG antibodies in applications such as immunoprecipitation, immunofluorescence, live cell imaging, biosensor assays, and protein purification.

Chromotek is now part of Proteintech

All ChromoTek Nanobody-based reagents are now available on ptglab.com and chromotek.com.

Nano-Traps

Nano-Traps are ideal for fast, reliable and efficient one-step immunoprecipitation and co-immunoprecipitation. Nano-Traps comprise of Nanobodies conjugated to beads.

Nano-Secondaries

Nano-Secondaries® are a novel class of secondary antibodies for higher resolution & cleaner images. Nano-Secondaries are Nanobodies conjugated to fluorescent dyes binding to conventional antibodies.

Chromobodies

Chromobodies® are fluorescent nanoprobes for real-time and live-cell imaging of intracellular endogenous proteins. Chromobodies are Nanobodies that are genetically fused to a fluorescent protein such as GFP.

Nano-Booster &
Nano-Label

ChromoTek's Nano-Boosters & Nano-Labels are very small fluorescent probes optimzed for confocal and super-resolution microscopy. They consist of Nanobodies conjugated to fluorescent dyes.

Nano-Caps

Nano-Caps® are optimized for effective and cost-efficient protein purification. Nano-Caps consist of Nanobodies conjugated to agarose.

Nano-CaptureLigands

Nano-CaptureLigands are optimized to specifically immobilize human, mouse, and rabbit antibodies to streptavidin/avidin. Nano-CaptureLigands are biotinylated Nanobodies.

Nanobodies/VHHs

Suitable for conjugation to dyes, biotin, beads, surfaces etc. via NHS ester reaction.

Spot-Tag System

ChromoTek's proprietary peptide-tag system for immunoprecipitation, immunofluorescence, and protein purification. Depending on application, Spot Nanobodies are conjugated to agarose, beads, or fluorescent dyes.

Immunoprecipitation

Nanobody based reagents show less background, i.e., no heavy and light antibody chains in SDS/PAGE and Western blot.

Immunofluorescence

Due to the small size of Nanobodies, higher image resolution can be achieved.

Live Cell Imaging

Chromobodies® visualize endogenous cellular structures and processes in live cells and real-time.

Western Blotting

Fluorescently labeled Nanobodies and antibodies allow for multiplexing.

Super-resolution Microscopy

The small size of Nanobodies enables better tissue penetration and decreases the distance between epitope and label.

Mass Spectrometry

Nanobody based tools enable for high reproducibility and low background.

Protein Purification

Affinity resins with Nanobodies allow for effective one-step protein purification.

Spot Capture and Detection System

Universal capture and detection system based on Spot-Nanobody for immunofluorescence, immunoprecipitation, protein purification, Western blotting, etc.

On-bead Enzyme Assays

The enzymatic activity can be assessed directly after immunoprecipitation from a cell lysate with Nanobodies.

High Content Analysis

Using Chromobodies, dynamic cellular changes can be visualized and monitored in real time.

Ligand Capture and Affinity Determination

Directed immobilization of antibodies for characterization of biomolecular interactions such as surface plasmon resonance (SPR) or bio-layer interferometry (BLI).

ELISA

An Enzyme-linked immunosorbent assay (ELISA) is method for the detection of a target molecule in a liquid sample.