The advantages of CoraLite direct staining for IF

Video highlights of how CoraLite® antibodies can streamline your multiplex immunofluorescence staining.

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This seminar was originally recorded on May 11, 2020 as part of the MetroFlow group’s corporate member seminar series. Dr. William Olds, Scientific Officer at Proteintech, highlights how CoraLite® antibodies can streamline your multiplex immunofluorescence staining.

Life After FC: Using CoraLite fluorescent dye-conjugated antibodies to simplify your multiplex imaging by Will Olds, PhD - Scientific Officer

CoraLite Fluorescent Dye-Conjugated Antibodies

Proteintech's popular monoclonal and polyclonal antibodies are now available directly conjugated with fluorescent dyes:  CoraLite488,  CoraLite594,  and CoraLite647. They act as perfect tools for immunofluorescence studies requiring multiplex co-labeling studies without the need for secondary antibodies:

• Can be used directly in immunofluorescence studies without the need for secondary antibodies

• Offer bright and long-lasting fluorescence

• Can be multiplexed with other CoraLite dyes and popular dyes (e.g. nuclear stains, DAPI, etc.)

Immunofluorescent analysis of ( 4% PFA) fixed rat brain tissue using 17490-1-AP (MAP2 antibody) at dilution of 1:100 and CoraLite594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The section was co-stained with 60190-1-Ig (GFAP antibody, green).

Immunofluorescent analysis of ( -20°C Ethanol) fixed oleic acid treated HepG2 cells using 15294-1-AP (ADRP/Perilipin 2 antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Red: CL555-phalloidin staining of F-actin.