Enter or paste the raw OD measurement data in a 12x8 matrix, leaving a space between each value as shown in the example data (you can easily overwrite this when inputting your own)
Paste your unedited OD data from a Word or Excel table into the text box below (Numeric format only)
Standard Concentrations Settings
For your standard concentration, enter the first (s1) or last (s6) standard concentration and select x2 or ÷2 depending on where you want the series calculations to start. A table with the standard series values will appear below this box once you click submit, check this is correct.
Initial standard concentration
Change Of Gradient
Ensure the “Well assignment” is laid out in the same way as the standards and samples of your experiment. You can use our suggested templates as for 7 standards or 11 standards or customise the layout with options to include blanks and controls.
Regression /Fitting model
Choose which regression model best fits your data. The standard concentrations and OD values will be automatically included from the previous tab. Our recommended model for most ELISA experiments is the four-parameter logistic (4PL) curve fit.
Standard OD Values
X data column
Most often, data fit best when plotted as logarithm (base 10). Select what is appropriate for your data set.
Y data column
X axis Name
Y axis Name
If your plate included blank replicates, tick the “deduct background” box to show the corrected values.
Click submit to see the standard curve. You can also click the download button on the top right of the graph to save this to your computer as an image. To use this curve to get results, you can hover your mouse over the curve to see the axis coordinate values
|X||Y-reaction value||Y-calculated value||Y-residual|
Use the ‘download’ button to export the table. Be mindful to adjust for dilution factors and replicate averages if not accounted for previously.
Total absorbance value (ODs):
|ID||Well(s)||Raw OD value(s)||Replicate Average||Infact OData||Conc.|