Immunohistochemistry

Secondary Antibodies

When choosing a secondary antibody for your IHC experiment, be mindful of the host species of your primary antibody and your preferred detection method. Proteintech offers Multi-rAb Recombinant Secondary Antibodies conjugated to a variety of fluorescent dyes and enzymes for fluorescent and chromogenic IHC, respectively.

Multi-rAb Recombinant Secondary Antibodies are finely crafted mixtures of recombinant goat monoclonal antibodies that recognize multiple complementary epitopes on the same IgG. Each recombinant clone in the final multiclonal mixture is carefully selected after rigorous characterization and screening to ensure high sensitivity, minimal cross-reactivity, and low background. Moreover, since Multi-rAb secondaries are generated using a recombinant platform they ensure high lot-to-lot consistency resulting in highly reproducible results.
Our Multi-rAb polymer HRP recombinant secondary antibodies enable highly sensitive, biotin-free detection of mouse or rabbit primary antibodies. Unlike traditional avidin-biotin based signal amplification methods, polymer HRP-based detection offers advantages of lower background and faster protocol with fewer steps. Therefore, we highly recommend using our Multi-rAb polymer HRP secondary antibodies for chromogenic IHC staining workflows. We also offer Multi-rAb recombinant secondary antibodies conjugated to various CoraLite Plus dyes for fluorescent IHC staining workflows.