CoraLite® Plus Fluorescent Dyes

Illuminate your research

CoraLite® Plus dyes, developed with SuperHydrophilic dPEG® technology, are advanced fluorophores with unmatched brightness and photostability

Immunofluorescence

CoraLite® Plus dye-conjugated primary antibodies offer a better signal-to-background ratio and higher sensitivity in immunofluorescence experiments.

View all CoraLite conjugated antibodies for IF

Flow Cytometry

CoraLite® Plus dye-conjugated primary antibodies for flow cytometry cover a wide range of wavelengths for easier multiplex panel design.

View all CoraLite conjugated antibodies for FC

Antibody Labeling

CoraLite® Plus dyes can be conjugated to any antibody from any supplier within 10 minutes using our FlexAble antibody labeling kit.

Learn more about FlexAble Antibody Labeling Kits

CoraLite® Plus dyes – A game-changing technology for fluorescence applications

Proteintech introduces CoraLite® Plus dyes, chemically engineered with SuperHydrophilic dPEG® groups for greater biocompatibility and high water solubility. This technology facilitates unmatched improvements in brightness and photostability. CoraLite® Plus dyes are spectrally similar to Alexa Fluor® dyes.

CoraLite® Plus makes low-abundance targets light up and every precious sample count. CoraLite® Plus dye-conjugated antibodies yield greater signal-to-noise ratios with minimal nonspecific binding. This results in higher specificity and intensity for fluorescent imaging, flow cytometry, and western blotting applications.

CoraLite® Plus dyes - Spectral Features

Dyes Excitation
(nm)
Emission
(nm)
Extinction Coefficient
(cm-1 M-1)
Spectrally similar dyes
CoraLite® Plus 405 399 422 35K Alexa Fluor® 405
CoraLite® Plus 488  493 522 73K Alexa Fluor® 488, FITC
CoraLite® Plus 555 554 570 150K Alexa Fluor® 555, Cy3, DyLight® 550
CoraLite® Plus 647 654 674 250K Alexa Fluor® 647, Cy5, DyLight® 650
CoraLite® Plus 750 755 780 240K Alexa Fluor® 750, Cy7, DyLight® 755

What is dPEG?

PEGylation is the process of covalently attaching polyethylene glycol (PEG) chains to various molecules like fluorophores to increase their hydrodynamic volume, water solubility and stability. dPEG® (discrete PEG) are molecules with a defined number of ethylene oxide units, thus giving them a specified chain length, molecular weight, and purity compared to traditional PEG with varying sizes and molecular weights. Varying sizes of PEG molecules leads to steric hindrance and other problems like binding/interaction site issues, which result in significant losses of intended activity.

CoraLite vs CoraLite Plus

Proteintech offers a range of antibodies conjugated to conventional sulfonated dyes, such as CoraLite®. The main difference between CoraLite® and CoraLite® Plus is that CoraLite® do not contain PEG molecules in their dye backbone.

Both classes of fluorophores have similar excitation-emission spectra and are comparable to Alexa Fluor® dyes.

Comparison between CoraLite and CoraLite Plus

Advantages of antibody conjugation with CoraLite® Plus dyes

Bioconjugation methods that use aliphatic or hydrophobic linkers for coupling biomolecules often cause problems in the final product due to nonspecific interactions between linker arms. SuperHydrophilic dPEG® technology can significantly improve final conjugate quality by improving specific interactions

Conventional Dye Conjugation

Conventional antibody dye Conjugation

  • Lower Degree of Labeling (DOL)
  • Higher DOL results in dye-dye quenching
  • Conjugates are more likely to aggregate or precipitate during labeling or storage
  • Conjugates have more nonspecific binding
  • Higher background

Antibody Conjugation with CoraLite® Plus

  • High Degree of Labeling (DOL)
  • Low levels of dye-dye quenching
  • No aggregation or precipitation of labeled molecules
  • Conjugates have less nonspecific binding
  • Greater signal-to-background ratio

dPEG® technology improves the conjugation efficiency and brightness of conventional dyes

SuperHydrophilic dPEG® technology enhances traditional dyes by decreasing negative charge and increasing hydrophilicity. This results in a greater degree of labeling and significantly increased brightness, even for traditional dyes such as FITC, Pacific Blue™, and Texas Red.

FITC vs FITC Plus degree of labeling
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    Conjugation with FITC Plus dye shows a higher degree of labeling compared to old-generation FITC.

FITC vs FITC Plus brightness
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    FITC Plus conjugated antibody displayed significantly greater quantum yield (and brightness) at all levels of DOL compared to FITC-conjugated antibody alone.

Proteintech also offers conventional dyes for flow cytometry application

  • The spectral properties of Atlantic Blue are similar to Pacific Blue™.
  • The spectral properties of Cardinal Red are similar to Texas Red.
Dyes Excitation
(nm)
Emission
(nm)
Extinction Coefficient
(cm-1 M-1)
Spectrally similar dyes
Atlantic Blue™ 404 458 19K Pacific Blue™
FITC/FITC Plus 495 524 76K Alexa Fluor® 488
PE 566 574 1,960K Alexa Fluor® 555, Cy3, DyLight® 550
Cardinal Red™ 592 611 80K Alexa Fluor® 594, Texas Red®
APC 650 661 700K Alexa Fluor® 647, Cy5, DyLight® 650

CoraLite Plus-conjugated antibodies show exceptional performance in various applications

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Alexa Fluor® is a registered trademark of Life Technologies Corporation.
DyLight® is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.
Pacific Blue™ is a trademark of Molecular Probes, Inc.
dPEG® is a trademark from Quanta Biodesign Ltd