Secondary Antibodies

Proteintech offers a comprehensive portfolio of secondary antibodies for detecting unconjugated primary antibodies in a wide range of applications including western blot, ELISA, immunofluorescence, immunohistochemistry, and flow cytometry.

Secondary Antibody Range


Multi-rAb Recombinant Secondary Antibodies are mixtures of precisely engineered recombinant monoclonal antibodies that recognize multiple complementary epitopes on the same IgG. Each recombinant clone in the final multiclonal secondary antibody mixture is carefully selected after rigorous characterization and screening to ensure the highest level of performance.

  • Combines the high sensitivity of polyclonals with the superior specificity of monoclonals
  • Minimal cross-reactivity with off-target species ​
  • Unlimited supply, high lot-to-lot consistency, and reproducibility of results throughout your project
  • Validated in WB, ELISA, IHC, ICC and Flow Cytometry​

Nano-Secondary Reagents

Immunofluorescence analysis of Hela cells stained with mouse IgG2a anti-Tubulin antibody (66240-1-Ig) and Nano-Secondary® alpaca anti-mouse IgG2a, recombinant VHH, CoraLite® Plus 647 (magenta). Nuclei were stained with DAPI (blue). Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.
Recombinantly generated alpaca VHHs against human, rabbit, and mouse IgGs suitable for achieving higher image resolution and better tissue penetration due to their smaller size.

Traditional Secondary Antibodies

Various lysates were subjected to SDS PAGE followed by western blot with 66031-1-Ig (alpha Tubulin antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. SA00001-1 (HRP-conjugated Affinipure Goat Anti-Mouse IgG(H+L) as secondary antibody.
Traditionally produced polyclonal secondaries offering high sensitivity and available in a wide selection of conjugates and species specificities.