Secondary Antibodies

Proteintech offers a comprehensive portfolio of highly sensitive secondary antibodies which have been validated to detect unconjugated primary antibodies.

These secondary antibodies are conjugated to various fluorescent dyes and enzymes suited for all your experimental needs. These products are tested to prove its performance in intended applications like Western blot, Immunofluorescence, Immunohistochemistry, Flow cytometry and ELISA.
ChromoTek’s Nano-Secondaries® are a novel class of secondary antibodies for higher resolution & cleaner images. They consist of Nanobodies/ VHHs that bind to primary antibodies with high affinity in a species and subtype specific manner. Nano-Secondaries are conjugated to Alexa Fluor® dyes.

Select your secondaries based on conjugates

Nano-Secondaries

  • One-step staining
  • Recombinant
  • Better resolution

Nano-Secondaries have certain advantages over the conventional secondaries.

  • Pre-incubation or parallel incubation of Nano-Secondaries with primary antibodies, i.e., one-step incubation, reduce incubation times and enhance multiplexing capabilities.
  • The recombinant process of developing Nano-Secondaries ensures high lot to lot consistency.
  • Smaller size of Nano-Secondaries has been proven to enhance image resolution in Super resolution Microscopy.

Multiplexed immunostaining of HeLa cells with two alpaca anti-mouse Nano-Secondaries and one anti-rabbit Nano-Secondary. Green: mouse IgG1 anti-COX4 + alpaca anti-mouse IgG1 VHH Alexa Fluor 488. Magenta: mouse IgG2b anti-Tubulin + alpaca anti-mouse IgG2b VHH Alexa Fluor 647. Yellow: rabbit anti-Lamin + alpaca anti-rabbit IgG VHH Alexa Fluor 568. Scale bar, 10 μm. Images were recorded at the Core Facility Bioimaging at the Biomedical Center, LMU Munich.

Featured Products

Recent Publications

Acta Biochim Biophys Sin (Shanghai)

Effect of Sox9 on TGF-β1-mediated atrial fibrosis

Hechuan Wang

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Secondary antibody selection

How to choose the best secondary antibody for your application

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Spectra viewer

Design and plan your experiments using our spectra viewer

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CoraLite® fluorescent dye-conjugated antibodies

CoraLite dyes have equivalent brightness to the Alexa Fluor® dyes.

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