Cytokines and Growth Factors

Human cell (HEK293) expressed cytokines and growth factors with high bioactivity, stability, lot-to-lot consistency, and native human conformation & post-translational modifications.

Our human expression system ensures that proteins have native conformation and post-translational modifications to optimize biological activity. No expression tags, xeno-free…just high quality proteins.

Animal component free

Endotoxin free

Xeno free

Tag free

Carrier free

HumanKine® recombinant proteins are created in HEK293 cells using animal free components

Proteins co-expressed in bacteria will not possess post- translational modifications, e.g., phosphorylation or glycosylation. For activity, many proteins require glycosylation and processing available exclusively in eukaryotic systems; specifically human systems for authentic human proteins.

All Humankine® recombinant proteins are produced in Proteintech's in-house cGMP grade laboratory adhering to strict quality control regulations.

  • Authentic human proteins

  • Native human conformation & post-translational modifications

  • High bioactivity & stability

  • Seamless transition from research to cGMP

Research Grade vs cGMP Grade

In order to facilitate a smooth transition from preclinical to clinical, Humankine® offers a wide range of Research Use Only (RUO) and cGMP grade recombinant proteins which are exclusively expressed and purified from HEK293 human cells. The RUO and cGMP products are both manufactured using the same expression systems, production process and operating procedures in a cGMP facility. This guarantees that there is no variability in the quality and performance of these products. ​

The key differentiator of our cGMP products from RUO is that we adhere to a rigorous quality process that is ISO13485 certified and cGMP compliant. The cGMP grade products come with extensive documentation, thorough QC testing and traceability. The RUO grade offers reliable products which are more cost-effective during early research and development. ​

cGMP Manufacturing Process

Proteintech cGMP cytokines and growth factor step process

Frequently Asked Questions

What is the stability of HumanKine proteins?

Unless specified otherwise in the product information sheet, our products are formulated to ensure the stability of lyophilized proteins at room temperature. It is advised to store lyophilized products at -20°C to -80°C. For reconstituted solutions of most products, short-term storage at 4°C is recommended. Extended storage of the protein solution should be at -20°C to -80°C. Keep in mind that each freeze/thaw cycle may lead to some protein denaturation, so it is not recommended to subject aliquots to multiple freeze/thaw cycle.

Why are some proteins reconstituted in acidic buffer?

Acid reconstitution buffer is especially important for proteins with a high isoelectric point, making the molecule hydrophobic at neutral pH. Failure to use the recommended buffer may result in incomplete dissolution of the cytokine or its potential loss on the surfaces of labware such as pipet tips, vials, and test tubes. Diluting the protein aliquots to the working concentration in the appropriate buffer for a specific application helps neutralize the small amount of acid added, ensuring the safe use of the protein with live cells.

Is it ok to replace Human Serum Albumin (HSA) with Bovine Serum Albumin (BSA) in reconstitution buffer?

Bovine serum albumin (BSA) can be used instead of Human Serum Albumin (HSA) in reconstitution buffer. We recommend to use recombinant HSA to keep the product animal component free. If your experiment will not be impacted by the presence of animal derived components, BSA containing buffer can be used for reconstitution.

Can HumanKine cytokines cross-react with mouse cells?

Except for a few cases, the majority of human cytokines exhibit activity on mouse cells. While many mouse cytokines can act on human cells, their specific activity might be lower compared to the corresponding human cytokine. Species specificity is observed in certain cytokines, such as interferons, GM-CSF, IL-3, and IL-4, which have minimal to no activity on non-homologous cells. In contrast, FGFs and neurotrophins are highly conserved, displaying robust activity on cells from various animal species.

How is HumanKine protein bioactivity calculated in international units(IU)?

International Unit (IU) values for our Humankine products are obtained by conducting multiple side-by-side comparisons with the WHO Reference Standard within our biological activity assay. This approach involves performing several comparison tests to account for potential outliers arising from variations in the assay, such as differences in the product, handling, assay protocol, and more. To learn more about international unit calculation, read our blog.

Why does my lyophilised protein vial appear to be empty?

The lyophilized product may have shifted from the vial's bottom during shipping/handling, scattering on the vial wall or cap. A firm tap on the bench or a brief spin in a centrifuge can help reposition the lyophilized product back to the vial's bottom. Certain pellets may present themselves as a minimal amount of material or a transparent film, attributed to the characteristics of the original buffer formulation. This appearance is typical for numerous proteins and is considered normal.

Resources

Mesenchymal Stem Cell

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Wnt Signaling

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Induced Pluripotent Stem Cell

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TGF-beta Signaling

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Haematopoietic Stem Cell

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