<strong>Migration patterns of Broad Range Prestained Protein Ladder in different electrophoretic conditions</strong><br>The apparent molecular weight of each protein (kDa) has been determined by calibration of each protein against an unstained protein ladder in specific electrophoresis conditions.
Note on apparent molecular weights: Depending upon the gel type used, the coupling of a charged dye molecule to a protein marker alters the overall charge of the protein and thus its mobility in a gel. This results in differences in observed molecular weight of the protein markers between different gel types as shown in figure.
2 ul protein ladders were loaded in the gel with concentration 8%-18%. After Electrophoresis, the proteins on the gel were transformed into the PVDF membrane. This image was taken by the Rio-Rad (ChemiDoc MP Imaging System) in the 550 nm (for Blue band) and 680 nm (for Red band) range.