Cerebral Organoids

HumanKine® Growth Factors are the perfect tool for your cerebral organoid research

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Cerebral organoids are a powerful tool to study neurodevelopment and implementing drug screening for neurological disorders. These structures are generated from induced or human pluripotent stem cells (iPSCs, hPSCs) grown in specialized media with growth factors.

HumanKine® recombinant cytokines and growth factors are authentic human proteins that are HEK293 cell expressed, and demonstrate greater activity than E. coli-expressed proteins. They are also tag and endotoxin-free.

Don't risk your organoid cultures with poor quality proteins: Choose HumanKine® growth factors

Target	Purity	Activity (EC50)	Source	Model Organoid	GMP-grade?
BMP4	>95%	1.5-9 ng/mL	HEK293	Hippocampal-Choroid Plexus	Yes
EGF	>95%	Active	HEK293	Cortical
FGFb	>95%	0.05-0.4 ng/mL	HEK293	Cortical, Cerebellar	Yes
FGF8	>95%	≤ 10 ng/mL	HEK293	Midbrain
GDNF	>95%	≤ 10 ng/mL	HEK293	Midbrain, Hypothalamus, Forebrain
SHH	>90%	≤ 350 ng/mL	HEK293	Midbrain
TGF	>95%	≤ 0.5 ng/mL	HEK293	Midbrain, Hypothalamus, Forebrain	Yes
WNT3A	>90%	2-17 ng/mL	HEK293	Midbrain, Hypothalamus, Forebrain	Yes

HumanKine® FGFb-TS protein

Recombinant human FGFbasic-TS (HZ-1285) stimulates dose-dependent proliferation of the HDFa human primary fibroblast cell line.

Know your organoids with neuronal and progenitor markers

Pax6 polyclonal antibody

SOX2 polyclonal antibody

TUJ1 monoclonal antibody

PAX6 (12323-1-AP, green) neuronal precursor cells (NPCs). Blue: DAPI. Provided by Bio Talentum Ltd., Hungary.

SOX2 (11064-1-AP, green)-positive cells in mouse brain tissue. Blue: DAPI

TUJ1/TUBB3 (66375-1-lg, green) hiPSC derived neuronal cultures (35 days old). Blue: DAPI.
Provided by BioTalentum Ltd., Hungary.

Antibodies for brain organoid characterization

Target	Reactivity	Host	Application	Cell/Tissue Type
CTIP2/BCL11B	Human, Mouse	Rabbit	WB, IP. IF	Deep-layer cortical neurons
DCX	Human, Mouse, Rat	Rabbit	WB, IHC, IF, ELISA	Developing neurons
DLX2	Human, Mouse	Rabbit	WB, IHC, IF, ELISA	NSCs, forebrain
EMX1	Human Mouse, Rat	Rabbit	WB, ELISA	Forebrain NSCs
EOMES/TBR2	Human, Mouse, Rat	Rabbit	WB, ELISA	Intermediate progenitors
FOXA2	Human, Mouse	Rabbit	WB, IP, IHC, IF, ChIP, ELISA	Midbrain dopaminergic neurons
FOXG1	Human, Mouse, Rat	Rabbit	WB, ELISA	Forebrain cells
FZD9	Human, Mouse, Rat	Rabbit	WB, IHC, ELISA	Cortical precursors, hippocampal neurons
GAD1	Human, Mouse, Rat	Rabbit	WB, IP, IHC, IF, ELISA	Ventral forebrain
KIRREL2	Human, Mouse, Rat	Rabbit	WB, ELISA	Cerebellar progenitors
LHX	Human	Rabbit	WB, ELISA	Ventral forebrain
NCAD	Human, Mouse Rat, Bovine, Canine, Gecko, Horse	Rabbit	WB, IP, IHC, IF, FC, CoIP, ELISA	NSCs, migrating neurons, neural plate
NELL2	Human	Rabbit	WB, IHC, IF, ELISA	NSCs, neurons
Nestin	Human, Mouse, Rat	Rabbit	WB, IHC, IF, ELISA	NSCs, progenitors GABAergic interneurons, oligodendrocytes
NKX2.1	Human, Mouse, Rat	Rabbit	WB, ELISA	ventral forebrain
OTX2	Human, Mouse, Rat, Chicken	Rabbit	WB, IP, IHC, IF, FC, ChIP, ELISA	Midbrain cells
PAX6	Human, Mouse, Rat	Rabbit	WB, IHC, IF, FC, ELISA	Radial glia/NSCs
PROX1	Human, Mouse, Rat	Rabbit	WB, IF, FC, ChIP, ELISA	Hippocampal neurons
Reelin	Human, Mouse	Rabbit	IHC, IF, ELISA	Pre-plate/Cajal-retzius cells
SATB2	Human, Mouse	Mouse	WB, IHC, ELISA	Cortical neurons across corpus callosum
SOX2	Human, Mouse Rat, Zebrafish	Rabbit	WB, IHC, IF, FC, ELISA	Radial glia/NSCs
TBR1	Human, Mouse, Rat	Rabbit	WB, IP, IHC, IF, ELISA	Pre-plate/Cajal-retzius cells
TH	Human, Mouse, Rat, Canine, Gerbil, Zebrafish	Rabbit	WB, IP, IHC, IF, ELISA	Midbrain NSCs
TTR	Human, Mouse	Rabbit	WB, IHC, IF, ELISA	Choroid plexus
TUJ1/TUBB3	Human, Mouse, Rat	Mouse	WB, IHC, FC, ELISA	Neurons
VGAT	C. elegans, Human, Mouse, Rat	Rabbit	WB, IP, IHC, IF, ELISA	GABAergic and glycinergic neurons
Vimentin	Human, Mouse, Rat, Bovine, Deer,Hamster, Pig	Rabbit	WB, IHC, IF, FC, ELISA	Radial glia/NSCs