NELL2 Polyclonal antibody

NELL2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 11268-1-AP

Synonyms

NEL like 2 (chicken), NEL like protein 2, Nel related protein 2, NELL2, NRP2

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Validation Data Gallery

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  WB analysis using 11268-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 11268-1-AP (NELL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: fetal human brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Product Information

11268-1-AP targets NELL2 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NELL2 fusion protein Ag1793
• Full Name: NEL-like 2 (chicken)
• Calculated Molecular Weight: 91 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC020544
• Gene Symbol: NELL2
• Gene ID (NCBI): 4753
• RRID: AB_10638004
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Nat Commun; A small secreted protein NICOL regulates lumicrine-mediated sperm maturation and male fertility; Authors - Daiji Kiyozumi
human	WB	Reprod Biol Endocrinol; Expression of NELL2/NICOL-ROS1 lumicrine signaling-related molecules in the human male reproductive tract; Authors - Daiji Kiyozumi
human	IHC, WB	Front Mol Biosci; An Extracellular Matrix-Based Signature Associated With Immune Microenvironment Predicts the Prognosis and Therapeutic Responses of Patients With Oesophageal Squamous Cell Carcinoma.; Authors - Hongpan Zhang
human	IF	Front Neurol; Tranylcypromine Causes Neurotoxicity and Represses BHC110/LSD1 in Human-Induced Pluripotent Stem Cell-Derived Cerebral Organoids Model.; Authors - Jing Huang
human	IHC	Oncol Rep; microRNA‑196a‑3p inhibits cell proliferation and promotes cell apoptosis by targeting ADP ribosylation factor 4 in diffuse large B‑cell lymphoma.; Authors - Jianhong Fu
human	IF	Neuroscience; PMID: 30599272; Authors - Fangkun Liu