NELL2 Polyclonal antibody

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  WB analysis using 11268-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 11268-1-AP (NELL2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 11268-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11268-1-AP (NELL2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 11268-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11268-1-AP (NELL2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

NELL2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11268-1-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

NEL like protein 2, Nel related protein 2, NEL-like protein 2, Nel-related protein 2, NRP2

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Tested Applications

• Positive WB detected in: fetal human brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Product Information

11268-1-AP targets NELL2 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1793

  Product name: Recombinant human NELL2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 443-740 aa of BC020544

  Sequence: ECAEGRHYCRENTMCVNTPGSFMCICKTGYIRIDDYSCTEHDECITNQHNCDENALCFNTVGGHNCVCKPGYTGNGTTCKAFCKDGCRNGGACIAANVCACPQGFTGPSCETDIDECSDGFVQCDSRANCINLPGWYHCECRDGYHDNGMFSPSGESCEDIDECGTGRHSCANDTICFNLDGGYDCRCPHGKNCTGDCIHDGKVKHNGQIWVLENDRCSVCSCQNGFVMCRRMVCDCENPTVDLFCCPECDPRLSSQCLHQNGETLYNSGDTWVQNCQQCRCLQGEVDCWPLPCPDVE

• Full Name: NEL-like 2 (chicken)
• Calculated Molecular Weight: 91 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC020544
• Gene Symbol: NELL2
• Gene ID (NCBI): 4753
• RRID: AB_10638004
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99435
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Nat Commun; A small secreted protein NICOL regulates lumicrine-mediated sperm maturation and male fertility; Authors - Daiji Kiyozumi
human	WB	Reprod Biol Endocrinol; Expression of NELL2/NICOL-ROS1 lumicrine signaling-related molecules in the human male reproductive tract; Authors - Daiji Kiyozumi
human	WB,IHC	Front Mol Biosci; An Extracellular Matrix-Based Signature Associated With Immune Microenvironment Predicts the Prognosis and Therapeutic Responses of Patients With Oesophageal Squamous Cell Carcinoma.; Authors - Hongpan Zhang
human	IF	Front Neurol; Tranylcypromine Causes Neurotoxicity and Represses BHC110/LSD1 in Human-Induced Pluripotent Stem Cell-Derived Cerebral Organoids Model.; Authors - Jing Huang
human	IHC	Oncol Rep; microRNA‑196a‑3p inhibits cell proliferation and promotes cell apoptosis by targeting ADP ribosylation factor 4 in diffuse large B‑cell lymphoma.; Authors - Jianhong Fu
human	IF	Discov Oncol; Comprehensive analysis of basement membrane-related genes showed that NELL2 is a new therapeutic target for colorectal cancer; Authors - Weiguo Wang