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  • KD/KO Validated

OTX2 Polyclonal antibody

OTX2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 13497-1-AP

Synonyms

Homeobox protein OTX2, MCOPS5, orthodenticle homeobox 2, Orthodenticle homolog 2, OTX2



Tested Applications

Positive WB detected inY79 cells, mouse retina tissue, rat retina tissue
Positive IP detected inY79 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse embryo tissue
Positive FC detected inhESC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13497-1-AP targets OTX2 in WB, IP, IHC, IF, FC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OTX2 fusion protein Ag4323
Full Name orthodenticle homeobox 2
Calculated molecular weight 297 aa, 32 kDa
Observed molecular weight 31-35 kDa
GenBank accession numberBC032579
Gene symbol OTX2
Gene ID (NCBI) 5015
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The orthodenticle homeobox 2, encoded by OTX2 gene, is a key transcription factor in developmental processes. In particular, it is required for the early specification of the brain and the embryonic development of sensory organs, including the, pineal gland, pituitary gland, inner part of the ear, eyes, and optic nerve. In later stages, it is important for maintaining intact retina and brain function. In addition, it acts as a transcriptional repressor and a gatekeeper of myogenic and neuronal differentiation in medulloblastoma cells. OTX2 binds to the MyoD1 core enhancer through its homeobox domain and the remarkable repressor activity exhibited by the homeobox domain renders OTX2 transcriptionally repressive

Protocols

Product Specific Protocols
WB protocol for OTX2 antibody 13497-1-APDownload protocol
IHC protocol for OTX2 antibody 13497-1-APDownload protocol
IF protocol for OTX2 antibody 13497-1-APDownload protocol
IP protocol for OTX2 antibody 13497-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

Unified rhombic lip origins of group 3 and group 4 medulloblastoma

Authors - Kyle S. Smith
mouseIF

Nature

Reversing a model of Parkinson's disease with in situ converted nigral neurons.

Authors - Hao Qian
mouseIF,ChIP

Science

Early life stress confers lifelong stress susceptibility in mice via ventral tegmental area OTX2.

Authors - Catherine J Peña
humanchip

Cancer Cell

Atypical Teratoid/Rhabdoid Tumors Are Comprised of Three Epigenetic Subgroups with Distinct Enhancer Landscapes.

Authors - Pascal D Johann
mouseWB,ChIP

Cell Rep

Otx2 and Oct4 Drive Early Enhancer Activation during Embryonic Stem Cell Transition from Naive Pluripotency.

Authors - Shen-Hsi Yang
  • KD Validated
mouseWB

Cell Rep

ZIC3 Controls the Transition from Naive to Primed Pluripotency.

Authors - Shen-Hsi Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

David (Verified Customer) (02-21-2022)

This antibody works very well for immunofluorescence on human brain.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1 to 200
  • Cell Tissue Type: Human brain
OTX2 Antibody Immunofluorescence validation (1 to 200 dilution) in Human brain (Cat no:13497-1-AP)