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OTX2 Polyclonal antibody
OTX2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 13497-1-AP
Validation Data Gallery
|Positive WB detected in||Y79 cells, mouse retina tissue, rat retina tissue|
|Positive IP detected in||Y79 cells|
|Positive IHC detected in||human gliomas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse embryo tissue|
|Positive FC detected in||HepG2 cells, hESC cells|
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
13497-1-AP targets OTX2 in WB, IP, IHC, IF, FC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, chicken|
|Host / Isotype||Rabbit / IgG|
|Immunogen||OTX2 fusion protein Ag4323|
|Full Name||orthodenticle homeobox 2|
|Calculated molecular weight||297 aa, 32 kDa|
|Observed molecular weight||31-35 kDa|
|GenBank accession number||BC032579|
|Gene ID (NCBI)||5015|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
The orthodenticle homeobox 2, encoded by OTX2 gene, is a key transcription factor in developmental processes. In particular, it is required for the early specification of the brain and the embryonic development of sensory organs, including the, pineal gland, pituitary gland, inner part of the ear, eyes, and optic nerve. In later stages, it is important for maintaining intact retina and brain function. In addition, it acts as a transcriptional repressor and a gatekeeper of myogenic and neuronal differentiation in medulloblastoma cells. OTX2 binds to the MyoD1 core enhancer through its homeobox domain and the remarkable repressor activity exhibited by the homeobox domain renders OTX2 transcriptionally repressive
|Product Specific Protocols|
|WB protocol for OTX2 antibody 13497-1-AP||Download protocol|
|IHC protocol for OTX2 antibody 13497-1-AP||Download protocol|
|IF protocol for OTX2 antibody 13497-1-AP||Download protocol|
|IP protocol for OTX2 antibody 13497-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Unified rhombic lip origins of group 3 and group 4 medulloblastoma
Reversing a model of Parkinson's disease with in situ converted nigral neurons.
Signal Transduct Target Ther
MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration
Early life stress confers lifelong stress susceptibility in mice via ventral tegmental area OTX2.
Atypical Teratoid/Rhabdoid Tumors Are Comprised of Three Epigenetic Subgroups with Distinct Enhancer Landscapes.
Otx2 and Oct4 Drive Early Enhancer Activation during Embryonic Stem Cell Transition from Naive Pluripotency.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
David (Verified Customer) (02-21-2022)
This antibody works very well for immunofluorescence on human brain.