Nestin Polyclonal antibody

Nestin Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

65

Reviews (3)

Cat no : 19483-1-AP

Synonyms

NES, Nestin

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Validation Data Gallery

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  WB analysis using 19483-1-AP

  Mouse brain lysates were subjected to SDS PAGE followed by western blot with 19483-1-AP (Nestin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 19483-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 19483-1-AP (NES antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 19483-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19483-1-AP (Nestin Antibody) at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human gliomas using 19483-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 19483-1-AP (Nestin Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human gliomas using 19483-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 19483-1-AP (Nestin Antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 19483-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19483-1-AP (Nestin Antibody) at dilution of 1:400 (under 40x lens).

• 

  IHC staining of mouse brain using 19483-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19483-1-AP (Nestin Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse brain using 19483-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19483-1-AP (Nestin Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of U-251 using 19483-1-AP

  Immunofluorescent analysis of U-251 cells using 19483-1-AP (Nestin antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of rat brain using 19483-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using 19483-1-AP (Nestin antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The section was co-stained with sox2 (66411-1-Ig, red).

• 

  IF Staining of SH-SY5Y using 19483-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 19483-1-AP (Nestin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 19483-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 19483-1-AP (Nestin antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: human kidney tissue, human gliomas tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: U-251 cells, rat brain tissue, SH-SY5Y cells, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 22 publications below
• IHC: See 9 publications below
• IF: See 41 publications below
• FC: See 1 publications below

Product Information

19483-1-AP targets Nestin in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: nestin
• Calculated molecular weight: 177 kDa
• Observed molecular weight: 170-180 kDa
• GenBank accession number: NM_006617
• Gene symbol: NES
• Gene ID (NCBI): 10763
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors.

Publications

Species	Application	Title
rat	IF	ACS Nano; Supramolecular Hydrogel Microspheres of Platelet-Derived Growth Factor Mimetic Peptide Promote Recovery from Spinal Cord Injury; Authors - Weidong Wu
human	WB,IF	Neuro Oncol; TRIM24 Promotes Stemness and Invasiveness of Glioblastoma Cells via Activating SOX2 Expression.; Authors - Lu-Hua Zhang
human,mouse	IF	Redox Biol; MG53 protein rejuvenates hUC-MSCs and facilitates their therapeutic effects in AD mice by activating Nrf2 signaling pathway.; Authors - Shanshan Ma
rat	IF	J Mater Chem B; Integration of genetically engineered virus nanofibers and fibrin to form injectable fibrous neuron-rich hydrogels and enable neural differentiation; Authors - Yingfan Chen
human	WB	PLoS Pathog; Human cytomegalovirus pUL97 upregulates SOCS3 expression via transcription factor RFX7 in neural progenitor cells; Authors - Xian-Zhang Wang
rat	IF	Neuropsychopharmacology; Exosomes from patients with major depression cause depressive-like behaviors in mice with involvement of miR-139-5p-regulated neurogenesis.; Authors - Ze-Xu Wei

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Udesh (Verified Customer) (02-24-2023)

Nestin in the upper lane Worked well at 1:1000 in 5% BSA, O/N, 4 degrees

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: U2OS Osteosarcoma cells

Nestin Antibody Western Blot validation (1:1000 dilution) in U2OS Osteosarcoma cells (Cat no:19483-1-AP)

FH

Balawant (Verified Customer) (08-23-2022)

this antibody is excellent for westernblot

• Applications: Western Blot
• Cell Tissue Type: colon tissue and colon cell line

FH

Silvia (Verified Customer) (09-30-2021)

Immunofluorescence (1:200) works well on NPC

• Applications: Immunofluorescence

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