Nestin Polyclonal antibody
Nestin Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 19483-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain tissue, rat brain tissue |
Positive IHC detected in | human kidney tissue, human gliomas tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | U-251 cells, rat brain tissue, SH-SY5Y cells, mouse brain tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 22 publications below |
IHC | See 9 publications below |
IF | See 41 publications below |
FC | See 1 publications below |
Product Information
19483-1-AP targets Nestin in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | nestin |
Calculated molecular weight | 177 kDa |
Observed molecular weight | 170-180 kDa |
GenBank accession number | NM_006617 |
Gene symbol | NES |
Gene ID (NCBI) | 10763 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors.
Protocols
Product Specific Protocols | |
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WB protocol for Nestin antibody 19483-1-AP | Download protocol |
IHC protocol for Nestin antibody 19483-1-AP | Download protocol |
IF protocol for Nestin antibody 19483-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
ACS Nano Supramolecular Hydrogel Microspheres of Platelet-Derived Growth Factor Mimetic Peptide Promote Recovery from Spinal Cord Injury | ||
Neuro Oncol TRIM24 Promotes Stemness and Invasiveness of Glioblastoma Cells via Activating SOX2 Expression. | ||
Redox Biol MG53 protein rejuvenates hUC-MSCs and facilitates their therapeutic effects in AD mice by activating Nrf2 signaling pathway. | ||
J Mater Chem B Integration of genetically engineered virus nanofibers and fibrin to form injectable fibrous neuron-rich hydrogels and enable neural differentiation | ||
PLoS Pathog Human cytomegalovirus pUL97 upregulates SOCS3 expression via transcription factor RFX7 in neural progenitor cells | ||
Neuropsychopharmacology Exosomes from patients with major depression cause depressive-like behaviors in mice with involvement of miR-139-5p-regulated neurogenesis. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Udesh (Verified Customer) (02-24-2023) | Nestin in the upper lane Worked well at 1:1000 in 5% BSA, O/N, 4 degrees
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FH Balawant (Verified Customer) (08-23-2022) | this antibody is excellent for westernblot
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FH Silvia (Verified Customer) (09-30-2021) | Immunofluorescence (1:200) works well on NPC
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