Nestin Polyclonal antibody

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  WB analysis using 19483-1-AP

  Mouse brain lysates were subjected to SDS PAGE followed by western blot with 19483-1-AP (Nestin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 19483-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 19483-1-AP (NES antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 19483-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 19483-1-AP (Nestin Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human gliomas using 19483-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 19483-1-AP (Nestin Antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 19483-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19483-1-AP (Nestin Antibody) at dilution of 1:400 (under 10x lens).

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  IHC staining of human kidney using 19483-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19483-1-AP (Nestin Antibody) at dilution of 1:400 (under 40x lens).

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  IF Staining of mouse kidney using 19483-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using Nestin antibody (19483-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U-251 using 19483-1-AP

  Immunofluorescent analysis of U-251 cells using 19483-1-AP (Nestin antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of SH-SY5Y using 19483-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using Nestin antibody (19483-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of SH-SY5Y using 19483-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 19483-1-AP (Nestin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of SH-SY5Y using 19483-1-AP

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Nestin Polyclonal antibody (19483-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Nestin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Cat No. 19483-1-AP

Publications(109)

Reviews (4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Synonyms

NES

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: human kidney tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse kidney tissue
• Positive IF/ICC detected in: SH-SY5Y cells, U-251 cells
• Positive FC (Intra) detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 35 publications below
• IHC: See 12 publications below
• IF: See 74 publications below
• FC: See 1 publications below

Product Information

19483-1-AP targets Nestin in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, monkey, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: nestin
• Calculated Molecular Weight: 177 kDa
• Observed Molecular Weight: 170-180 kDa
• GenBank Accession Number: NM_006617
• Gene Symbol: Nestin
• Gene ID (NCBI): 10763
• RRID: AB_10644440
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P48681
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors. Nestin can be detected as 220-240 kDa and 240-280 kDa. Studies point toward nestin heavy posttranslational modifications as possibly serving particular functions in podocytes and in a few other particular cell types(PMID: 17210924).

Publications

Species	Application	Title
rat	IF	ACS Nano; Supramolecular Hydrogel Microspheres of Platelet-Derived Growth Factor Mimetic Peptide Promote Recovery from Spinal Cord Injury; Authors - Weidong Wu
monkey	IF	Int J Surg; Stereotactically intracerebral transplantation of neural stem cells for ischemic stroke attenuated inflammatory responses and promoted neurogenesis: an experimental study with monkeys; Authors - Yi-Fan Liu
	IF	J Biomed Sci; Genetic and pharmacologic p32-inhibition rescue CHCHD2-linked Parkinson's disease phenotypes in vivo and in cell models; Authors - Murni Tio
human	WB,IF	Neuro Oncol; TRIM24 Promotes Stemness and Invasiveness of Glioblastoma Cells via Activating SOX2 Expression.; Authors - Lu-Hua Zhang
human,mouse	IF	Redox Biol; MG53 protein rejuvenates hUC-MSCs and facilitates their therapeutic effects in AD mice by activating Nrf2 signaling pathway.; Authors - Shanshan Ma
mouse	IF	Acta Biomater; Induced pluripotent stem cell-derived neural stem cells promote bone formation in mice with calvarial defects; Authors - Yukari Shiwaku

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Reyes (Verified Customer) (03-18-2024)

I tested nestin (in red) in human brain cortical sections obtained from pediatric patients, and the result was inconclusive, as it looks like ther is a halo around my DAPI, and there is no clear nestin marking in any cell type in the tissue.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

Nestin Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:19483-1-AP)

FH

Udesh (Verified Customer) (02-24-2023)

Nestin in the upper lane Worked well at 1:1000 in 5% BSA, O/N, 4 degrees

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: U2OS Osteosarcoma cells

Nestin Antibody Western Blot validation (1:1000 dilution) in U2OS Osteosarcoma cells (Cat no:19483-1-AP)

FH

Balawant (Verified Customer) (08-23-2022)

this antibody is excellent for westernblot

• Applications: Western Blot
• Cell Tissue Type: colon tissue and colon cell line

FH

Silvia (Verified Customer) (09-30-2021)

Immunofluorescence (1:200) works well on NPC

• Applications: Immunofluorescence