Nestin Polyclonal antibody

Nestin Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 19483-1-AP

Synonyms

NES, Nestin



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman kidney tissue, human gliomas tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU-251 cells, rat brain tissue, SH-SY5Y cells, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

19483-1-AP targets Nestin in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name nestin
Calculated molecular weight 177 kDa
Observed molecular weight 170-180 kDa
GenBank accession numberNM_006617
Gene symbol NES
Gene ID (NCBI) 10763
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors.

Protocols

Product Specific Protocols
WB protocol for Nestin antibody 19483-1-APDownload protocol
IHC protocol for Nestin antibody 19483-1-APDownload protocol
IF protocol for Nestin antibody 19483-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Neuro Oncol

TRIM24 Promotes Stemness and Invasiveness of Glioblastoma Cells via Activating SOX2 Expression.

Authors - Lu-Hua Zhang
human,mouseIF

Redox Biol

MG53 protein rejuvenates hUC-MSCs and facilitates their therapeutic effects in AD mice by activating Nrf2 signaling pathway.

Authors - Shanshan Ma
ratIF

Neuropsychopharmacology

Exosomes from patients with major depression cause depressive-like behaviors in mice with involvement of miR-139-5p-regulated neurogenesis.

Authors - Ze-Xu Wei
humanIF

Cancers (Basel)

Spreading of Alpha Synuclein from Glioblastoma Cells towards Astrocytes Correlates with Stem-like Properties.

Authors - Larisa Ryskalin
ratWB,IF

J Neuroinflammation

Apelin alleviated neuroinflammation and promoted endogenous neural stem cell proliferation and differentiation after spinal cord injury in rats.

Authors - Qing Liu
humanWB

Aging (Albany NY)

The Interleukin-33/ST2 axis promotes glioma mesenchymal transition, stemness and TMZ resistance via JNK activation.

Authors - Lin Lin

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Balawant (Verified Customer) (08-23-2022)

this antibody is excellent for westernblot

  • Applications: Western Blot
  • Cell Tissue Type: colon tissue and colon cell line
FH

Silvia (Verified Customer) (09-30-2021)

Immunofluorescence (1:200) works well on NPC

  • Applications: Immunofluorescence