DCX Polyclonal antibody

DCX Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 13925-1-AP


DBCN, DC, DCX, doublecortin, Doublin, Lis X, Lissencephalin X, LISX, SCLH, XLIS

Tested Applications

Positive WB detected inSH-SY5Y cells, fetal human brain tissue
Positive IHC detected inhuman gliomas tissue, mouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13925-1-AP targets DCX in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DCX fusion protein Ag4945
Full Name doublecortin
Calculated Molecular Weight 402 aa, 45 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC027925
Gene Symbol DCX
Gene ID (NCBI) 1641
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DCX (Doublecortin) is a microtubule-associated protein that is essential for normal brain development. DCX is expressed in migrating neurons throughout the central and peripheral nervous system during embryonic and postnatal development. Anti-DCX antibodies are widely used for detection of neurogenesis. It's also a common neuron marker since its expression is specific to neurons.


Product Specific Protocols
WB protocol for DCX antibody 13925-1-APDownload protocol
IHC protocol for DCX antibody 13925-1-APDownload protocol
IF protocol for DCX antibody 13925-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Sampath (Verified Customer) (12-02-2019)

It worked very well brain samples and gave me a strong signal for DCX at 1:1000 dilution for western blots. I had to increase the dilution to 1:2000. This is the antibody I was looking for. If you are looking for DCX antibody, I will recommend this.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000

Sheed (Verified Customer) (01-26-2019)

Works well with Edu click reaction

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse

Eleonora (Verified Customer) (12-11-2018)

The antibody works well, but it has too much interaction with the protein ladder (I have used Fisher BioReagent EZ-RUN).

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:600
  • Cell Tissue Type: cortex (right) and hippocampus (left) mouse 3 weeks
DCX Antibody Western Blot, validation (1:600 dilution) in cortex (right) and hippocampus (left) mouse 3 weeks (Cat no:13925-1-AP)