FOXG1 Polyclonal antibody

FOXG1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 12764-1-AP

Synonyms

BF 1, BF 2, BF1, BF2, Brain factor 1, Brain factor 2, FHKL3, FKH2, FKHL1, FKHL2, FKHL3, FKHL4, forkhead box G1, Forkhead box protein G1, Forkhead box protein G1A, Forkhead box protein G1B, Forkhead box protein G1C, Forkhead related protein FKHL1, Forkhead related protein FKHL2, Forkhead related protein FKHL3, FOXG1, FOXG1A, FOXG1B, FOXG1C, HBF 1, HBF 2, HBF 3, HBF G2, HBF2, HFK1, HFK2, HFK3, KHL2, QIN

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Validation Data Gallery

• 

  WB analysis of mouse testis using 12764-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12764-1-AP (FOXG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 12764-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 12764-1-AP (FOXG1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12764-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12764-1-AP (FOXG1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12764-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12764-1-AP (FOXG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse testis tissue, mouse brain tissue, SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12764-1-AP targets FOXG1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FOXG1 fusion protein Ag3426
• Full Name: forkhead box G1
• Calculated Molecular Weight: 489 aa, 52 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC035020
• Gene Symbol: FOXG1
• Gene ID (NCBI): 2290
• RRID: AB_2231894
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Forkhead Box G1 (FOXG1) is a member of the Forkhead family of genes with non-redundant roles in brain development, where alteration of this gene's expression significantly affects the formation and function of the mammalian cerebral cortex. FOXG1 haploinsufficiency in humans is associated with prominent differences in brain size and impaired intellectual development noticeable in early childhood, while homozygous mutations are typically fatal.