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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human FGF-8b protein

Activity(Primary)

10-60 ng/mL EC50 in NIH 3T3 in defined media or 0.7-4 ng/mL EC50 in Balb/c 3T3.

Species Reactivity

human,mouse,pig

Purity

>95%

Cat no : HZ-1103

Synonyms

AIGF, Androgen induced growth factor, FGF 8, FGF8, FGF-8b, Fibroblast growth factor 8, HBGF 8, KAL6

Validation Data Gallery

  • Purity of recombinant human FGF-8b was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

    Purity of recombinant human FGF-8b was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

  • The activity was determined by the dose-dependent stimulation of the proliferation of the Balb/3T3 cell line using the Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

    The activity was determined by the dose-dependent stimulation of the proliferation of the Balb/3T3 cell line using the Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

  • Recombinant human FGF-8b (HZ-1218) stimulates dose-dependent proliferation of the NIH/3T3 mouse fibroblast cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. NIH/3T3 cells were serum starved in 0.02% FBS with 1 ug/mL heparin during treatment with increasing concentrations of recombinant human FGF-8b for 72hrs. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 10-60 ng/mL.

    Recombinant human FGF-8b (HZ-1218) stimulates dose-dependent proliferation of the NIH/3T3 mouse fibroblast cell line. Viable cell number was quantitatively assessed by Prestoblue Cell Viability Reagent. NIH/3T3 cells were serum starved in 0.02% FBS with 1 ug/mL heparin during treatment with increasing concentrations of recombinant human FGF-8b for 72hrs. The EC50 was determined using a 4- parameter non-linear regression model. The EC50 values range from 10-60 ng/mL.

  • Human induced pluripotent cells(iPSCs) were differentiated to dopaminergic neurons using HumanKine growth factors (GDNF-HZ-1311 and FGF8B- HZ-1103). The differentiation was confirmed by detection of expression of TAU(green), Alpha-Synuclein(red) and Thyrosine hydoxylase(TH)(yellow) in immunofluorescence.(Credits- Credits- Alessandro Bellapianta Johannes Kepler Universitat)

    Human induced pluripotent cells(iPSCs) were differentiated to dopaminergic neurons using HumanKine growth factors (GDNF-HZ-1311 and FGF8B- HZ-1103). The differentiation was confirmed by detection of expression of TAU(green), Alpha-Synuclein(red) and Thyrosine hydoxylase(TH)(yellow) in immunofluorescence.(Credits- Credits- Alessandro Bellapianta Johannes Kepler Universitat)

Technical Specifications

GeneID 2253
Species Human
Expression HEK293
Activity(Primary) 10-60 ng/mL EC50 in NIH 3T3 in defined media or 0.7-4 ng/mL EC50 in Balb/c 3T3.
Activity(Secondary)0.7-4 ng/mL EC50 in Balb/c 3T3.
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP55075
Molecular Mass 30 to 45 kDa reduced and non-reduced, monomer, glycosylated
Formulation 10 mM Tris-HCl pH 7.4 + 800 mM NaCl, See Certificate of Analysis for details
Species Reactivity human,mouse,pig

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile water containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

Fibroblast growth factor 8 (FGF8), also known as AIGF and HBGF-8, belongs to the FGF family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion (PMID: 11276432; 15863033). FGF8 stimulates growth in an autocrine manner in normal individuals but mediates hormonal action during tumorigenesis (PMID 17512240). Defects in FGF8 cause Kallmann syndrome type 6 (KAL6) and idiopathic hypogonadotropic hypogonadism (IHH) (PMID 18596921).

Synonyms

AIGF, Androgen induced growth factor, FGF 8, FGF8, FGF-8b, Fibroblast growth factor 8, HBGF 8, KAL6

Publications

SpeciesTitle
human

Stem Cells Transl Med

Mitomycin-C treatment during differentiation of induced pluripotent stem cell-derived dopamine neurons reduces proliferation without compromising survival or function in vivo.

Authors - Benjamin M Hiller
View Article
pig

NMR Biomed

MRI study of cryoinjury infarction in pig hearts: i. Effects of intrapericardial delivery of bFGF/VEGF embedded in alginate beads.

Authors - YYang
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Alessandro (Verified Customer) (07-27-2022)

eccificient dopaminergic differentiation

  • Applications: Cell culture
  • Cell Tissue Type: NPCs