EOMES/TBR2 Polyclonal antibody

EOMES/TBR2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28316-1-AP

Synonyms

EOMES, EOMES/TBR2, Eomesodermin homolog, T box brain protein 2, T brain 2, TBR 2, TBR2

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Validation Data Gallery

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  WB analysis using 28316-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28316-1-AP (EOMES antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 28316-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 28316-1-AP (EOMES antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28316-1-AP targets EOMES/TBR2 in WB, ELISA applications and shows reactivity with Human, Mouse, rat samples.

• Tested Reactivity: Human, Mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: EOMES/TBR2 fusion protein Ag27634
• Full Name: eomesodermin homolog (Xenopus laevis)
• Calculated Molecular Weight: 73 aa
• Observed Molecular Weight: 70-72 kDa
• GenBank Accession Number: NM_001278182
• Gene Symbol: EOMES
• Gene ID (NCBI): 8320
• RRID: AB_2881110
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

EOMES/TRB2 (also named Eomesodermin/Tbr2) encode T-box transcription factor expressed highly in the intermediate progenitor stage of the developing neuron. During migration of the neurons, radial glia divide and migrate towards the surface of the cerebral cortex, there are three stages of cellular development: radial glia, intermediate progenitors, and postmitotic projection neurons. Radial glia express Pax6, while intermediate progenitor cells express Eomesodermin/Tbr2, and postmitotic projection neurons express Tbr1 (PMID:15634788; PMID:16320258). This process also called neurogenesis, and demonstrated that Eomesodermin/Tbr2 has been implicated in neurodevelopment. According to the mouse model, several articles showed that EOMES/TRB2 KO mice having proliferating cells decreased and having smaller upper cortical layers and a smaller sub ventricular zone in the brain (PMID:18940588). What's more, Eomesodermin/Tbr2 has also been found participating in immune response (PMID:20713880).