Vimentin Polyclonal antibody

• Featured Product
• KD/KO Validated

Vimentin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, CoIP, RIP, ELISA, Cell treatment

Conjugate

Unconjugated

Publications

1700

Reviews (10)

Cat no : 10366-1-AP

Synonyms

VIM

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Validation Data Gallery

• 

  WB analysis using 10366-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10366-1-AP (Vimentin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat cells using 10366-1-AP

  WB result of Vimentin antibody (10366-1-AP, 1:4000) with si-Control and si-Vimentin transfected Jurkat cells.

• 

  WB analysis of NIH/3T3 using 10366-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10366-1-AP (VIM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 10366-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10366-1-AP (VIM Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 10366-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10366-1-AP (Vimentin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 10366-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 10366-1-AP (Vimentin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 10366-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 10366-1-AP (Vimentin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 10366-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 10366-1-AP (Vimentin antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 10366-1-AP

  Immunohistochemical analysis of paraffin-embedded normal colon slide using 10366-1-AP (Vimentin antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10366-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10366-1-AP (Vimentin antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 10366-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10366-1-AP (Vimentin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 10366-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10366-1-AP (Vimentin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 10366-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10366-1-AP (VIM antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of human breast cancer using 10366-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Vimentin antibody (10366-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human kidney using 10366-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Vimentin antibody (10366-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human breast cancer using 10366-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Vimentin antibody (10366-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human kidney using 10366-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Vimentin antibody (10366-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 10366-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using 10366-1-AP (Vimentin antibody) at dilution of 1:400 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 10366-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 10366-1-AP (Vimentin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 10366-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Vimentin antibody (10366-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• 

  IF Staining of HepG2 using 10366-1-AP

  Immunofluorescent analysis of HepG2 cells, using VIM antibody 10366-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HeLa using 10366-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Vimentin (10366-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of mMSCs using 10366-1-AP

  1x10^6 mMSCs cells were intracellularly stained with 0.5 ug Vimentin Polyclonal antibody (10366-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HepG2 cells, NIH/3T3 cells, mouse heart tissue, Jurkat cells, HeLa cells
• Positive IHC detected in: human liver cancer tissue, human breast cancer tissue, human hepatocirrhosis tissue, human liver tissue, human normal colon, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human breast cancer tissue, human kidney tissue
• Positive IF-Fro detected in: mouse brain tissue
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HeLa cells, mMSCs cells

Recommended dilution

• Western Blot (WB): WB : 1:20000-1:100000
• Immunohistochemistry (IHC): IHC : 1:2500-1:10000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)-FRO: IF-FRO : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 1416 publications below
• IHC: See 269 publications below
• IF: See 344 publications below
• IP: See 9 publications below
• ELISA: See 1 publications below
• FC: See 2 publications below
• CoIP: See 5 publications below
• RIP: See 1 publications below

Product Information

10366-1-AP targets Vimentin in WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, CoIP, RIP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, bovine, hamster, sheep, goat, deer
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Vimentin fusion protein Ag0489
• Full Name: vimentin
• Calculated Molecular Weight: 466 aa, 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC000163
• Gene Symbol: VIM
• Gene ID (NCBI): 7431
• RRID: AB_2273020
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. Isoforms of vimentin (49 kDa and 60 kDa) had also been reported. (PMID: 8640945, 22728585).

Publications

Species	Application	Title
human	WB,IF	Nat Cancer; Spatial single-cell protein landscape reveals vimentinhigh macrophages as immune-suppressive in the microenvironment of hepatocellular carcinoma; Authors - Xinyao Qiu
human	WB	Cell Res; The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8.; Authors - Jia-Yuan Zhang
human	WB	ACS Nano; Deep-Learning-Based Nanomechanical Vibration for Rapid and Label-Free Assay of Epithelial Mesenchymal Transition; Authors - Wenjie Wu
mouse	IF	Cell Host Microbe; Gut microbiome dysbiosis contributes to abdominal aortic aneurysm by promoting neutrophil extracellular trap formation; Authors - Zhenyu Tian
human		Adv Sci (Weinh); PMID: 31728273; Authors - Qingming Tang
	IHC	Circ Res; Glutamyl-Prolyl-tRNA Synthetase Regulates Proline-Rich Pro-Fibrotic Protein Synthesis During Cardiac Fibrosis.; Authors - Jiangbin Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Vernon (Verified Customer) (01-29-2024)

Western blot signal is great with 30 sec exposure secondary antibody 1:5000 1hr. at room temp

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Ewing sarcoma cell line

Vimentin Antibody Western Blot validation (1:2000 dilution) in Ewing sarcoma cell line (Cat no:10366-1-AP)

FH

Tobias (Verified Customer) (06-16-2023)

Works well in IF

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200

FH

Emma (Verified Customer) (02-22-2022)

Works well by IF on FFPE prostate tissue. (Tris-EDTA antigen retrieval).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Prostate

FH

Yuki (Verified Customer) (01-07-2022)

Consistently works antibody

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:150
• Cell Tissue Type: gut duct tissue

Vimentin Antibody Immunofluorescence validation (1:150 dilution) in gut duct tissue (Cat no:10366-1-AP)

FH

Hala (Verified Customer) (07-08-2021)

Works veryyyy gooddd

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000 - 1/2000
• Cell Tissue Type: hepG2 transduced with TDO2 gene, liver cancer cells

FH

Hala (Verified Customer) (04-11-2021)

very good antibody, works very well

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: hepG2 transduced with TDO2 gene

Vimentin Antibody Western Blot validation (1/1000 dilution) in hepG2 transduced with TDO2 gene (Cat no:10366-1-AP)

FH

wei (Verified Customer) (04-27-2020)

I tried in C2C12 cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: C2C12

Vimentin Antibody Western Blot validation (1:1000 dilution) in C2C12 (Cat no:10366-1-AP)

FH

Patricia (Verified Customer) (02-25-2020)

Works well.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Ovarian cancer

FH

Kishor (Verified Customer) (01-30-2019)

Good results in western blotting but I could not see consistent results in IF

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: H69, HUCCT-1

FH

Jaime (Verified Customer) (01-24-2018)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/200
• Cell Tissue Type: Mouse ventricle frozen sections

Vimentin Antibody Immunofluorescence validation (1/200 dilution) in Mouse ventricle frozen sections (Cat no:10366-1-AP)

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