BCL11B Polyclonal antibody

BCL11B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (1)

Cat no : 55414-1-AP

Synonyms

COUP TF interacting protein 2, BCL-11B, B-cell lymphoma/leukemia 11B, B-cell CLL/lymphoma 11B, B cell lymphoma/leukemia 11B

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Validation Data Gallery

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  WB analysis using 55414-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55414-1-AP (BCL11B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 55414-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55414-1-AP (BCL11B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 55414-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 55414-1-AP (BCL11B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 55414-1-AP

  IP result of anti-BCL11B (IP:55414-1-AP, 4ug; Detection:55414-1-AP 1:5000) with Jurkat cells lysate 1320 ug.

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  IHC staining of mouse brain using 55414-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55414-1-AP (BCL11B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 55414-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 55414-1-AP (BCL11B antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse thymus tissue, MOLT-4 cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

55414-1-AP targets BCL11B in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: B-cell CLL/lymphoma 11B (zinc finger protein)
• Calculated Molecular Weight: 96 kDa
• Observed Molecular Weight: 116 kDa
• GenBank Accession Number: NM_022898
• Gene Symbol: BCL11B
• Gene ID (NCBI): 64919
• RRID: AB_11182609
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BCL11B, also named CTIP2, RIT1, CTIP-2, ZNF856B, and hRIT1-alpha, is a tumor-suppressor protein involved in T-cell lymphomas. It may function on the P53-signaling pathway. BCL11B is a key regulator of both differentiation and survival during thymocyte development. It depresses transcription through direct, TFCOUP2-independent binding to a GC-rich response element. This antibody is specific to BCL11B. The calculated molecular weight of BCL11B is 95 kDa but the modified BCL11B protein is about 116 kDa.

Publications

Species	Application	Title
human	WB	Arch Virol; Increased expression of BCL11B and its recruited chromatin remodeling factors during highly active antiretroviral therapy synergistically represses the transcription of human immunodeficiency virus type 1 and is associated with residual immune activation.; Authors - Juan Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Alessandro (Verified Customer) (12-09-2023)

Good for ICC on subcerebral neurons

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: ROs