HumanKine® recombinant human TGF beta 1 protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

Typically ≤ 0.5 ng/mL

Specific Activity

minimally 1.00 x 10⁷ IU/mg

Species Reactivity

human,mouse,goat,pig,rat

Purity

>95%

Publications

205

Reviews (3)

Cat no : HZ-1011

Synonyms

CED, DPD1, LAP, TGF B1, TGF beta 1, tgf beta1, TGFB, TGFB1, TGFbeta, tgf-beta1

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Validation Data Gallery

• 

  Purity of recombinant TGF beta 1 was determined by SDS-polyacrylamide gel electrophoresis. The protein was resolved in an SDS-polyacrylamide gel in reducing and non-reducing conditions followed by staining with Comassie blue.

• 

  Recombinant human TGF beta 1 (HZ-1011) inhibits IL-4 induced proliferation of the HT-2 mouse cell line. HT-2 cells are Balb/c spleen cells activated by sheep erythrocytes in the presence of IL-2. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-2 cells were treated with increasing concentrations of recombinant TGF beta 1 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.01-0.17 ng/mL.

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  Human iPSC-derived microglia (white) residing within an in vivo brain-like organoid environment (blue) derived using HumanKine growth factors (TGF beta 1- HZ-1011, BMP4- HZ-1045, and Thrombopoietin HZ-1248).(Credits- Credit: Simon T. Schafer & Monique Pena, Technical University of Munich, Center for Organoid Systems)

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Technical Specifications

• GeneID: 7040
• Species: Human
• Expression: HEK293
• Activity(Primary): Typically ≤ 0.5 ng/mL
• Specific Activity: minimally 1.00 x 10^7 IU/mg
• Purity: >95%
• Endotoxin: <1 EU/µg
• Accession Number: P01137
• Molecular Mass: 13 kDa reduced, 25 kDa non-reduced, homodimer, non-glycosylated
• Formulation: 50 mM NaOAc pH 3.7, See Certificate of Analysis for details
• Species Reactivity: human,mouse,goat,pig,rat

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

• Reconstitution: Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 4 mM HCl containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

Whitepapers

Effective Polarization of Human Th17 Cells with Biologicaly Relevant HumanKine® TGFβ1, TGFβ2, and TGFβ3 Expressed in Human Cells

Effective Differentiation of Th17 Cells With HumanKine® Cytokines Expressed in Human Cells

Efficient Induction of Th17 Cells with HumanKine® TGFβ1

Background

TGFB, also named as LAP and TGFB1, is a multifunctional peptide that controls proliferation, and cell differentiation. TGF acts synergistically with TGFA in inducing transformation process. It also acts as a negative autocrine growth factor. Dysregulation of TGFB activation and signaling may result in apoptosis. Many cells synthesize TGFB having specific receptors for it. TGFB positively and negatively regulates many other growth factors. It plays an important role in bone remodeling being a potent stimulator of an osteoblastic bone formation (PMID: 11586292).

Synonyms

CED, DPD1, LAP, TGF B1, TGF beta 1, tgf beta1, TGFB, TGFB1, TGFbeta, tgf-beta1

Publications

Species	Title
human	Nature; Paracrine signalling by cardiac calcitonin controls atrial fibrogenesis and arrhythmia.; Authors - Lucia M Moreira
human	Nat Biotechnol; Multiple targets of miR-302 and miR-372 promote reprogramming of human fibroblasts to induced pluripotent stem cells.; Authors - DeepaSubramanyam
human	Immunity; Nuclear localization of STING1 competes with canonical signaling to activate AHR for commensal and intestinal homeostasis; Authors - Ruoxi Zhang
human	Cell Metab; Microglial lipid droplet accumulation in tauopathy brain is regulated by neuronal AMPK; Authors - Yajuan Li
human	Nat Cell Biol; A20 promotes metastasis of aggressive basal-like breast cancers through multi-monoubiquitylation of Snail1.; Authors - Ji-Hyung Lee
human	ACS Nano; Deep-Learning-Based Nanomechanical Vibration for Rapid and Label-Free Assay of Epithelial Mesenchymal Transition; Authors - Wenjie Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

MARIA (Verified Customer) (11-13-2024)

We applied TGFb to the stem cell culture, together with other reagents. It worked fine, we didnt have any problem

• Applications: Cell culture
• Cell Tissue Type: Stem cells

FH

Linshan (Verified Customer) (05-23-2024)

This product has an effect in my experiment. I would recommend it.

• Applications: Other

FH

Sebastian (Verified Customer) (10-25-2023)

Used to stimulate cardiac fibroblasts. Worked well.

• Applications: Cell culture
• Primary Antibody Dilution: -
• Cell Tissue Type: Cardiac Fibroblasts