HumanKine® recombinant human TGF beta 1 protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity

Typically ≤ 0.5 ng/mL EC50

Species Reactivity

human,mouse

Purity

>95%

Publications

122

Cat no : HZ-1011

Synonyms

CED, DPD1, LAP, TGF B1, TGF beta 1, tgf beta1, TGFB, TGFB1, TGFbeta, tgf-beta1

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Validation Data Gallery

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  The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

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  The activity was determined by the dose-dependent inhibition of IL-4 induced proliferation of mouse HT-2 cells (BALB/c spleen activated by sheep erythrocytes in the presence of IL-2) using Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

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Technical Specifications

• GeneID: 7040
• Species: Human
• Expression: HEK293
• Activity: Typically ≤ 0.5 ng/mL EC50
• Purity: >95%
• Endotoxin: <1 EU/µg
• Accession Number: P01137
• Molecular Mass: 25 kDa, homodimer, nonglycosylated
• Formulation: 50 mM NaOAc pH 4.5, See Certificate of Analysis for details
• Species Reactivity: human,mouse

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 4 mM HCl containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Whitepapers

Effective Polarization of Human Th17 Cells with Biologicaly Relevant HumanKine® TGFβ1, TGFβ2, and TGFβ3 Expressed in Human Cells

Effective Differentiation of Th17 Cells With HumanKine® Cytokines Expressed in Human Cells

Efficient Induction of Th17 Cells with HumanKine® TGFβ1

Background

TGFB, also named as LAP and TGFB1, is a multifunctional peptide that controls proliferation, and cell differentiation. TGF acts synergistically with TGFA in inducing transformation process. It also acts as a negative autocrine growth factor. Dysregulation of TGFB activation and signaling may result in apoptosis. Many cells synthesize TGFB having specific receptors for it. TGFB positively and negatively regulates many other growth factors. It plays an important role in bone remodeling being a potent stimulator of an osteoblastic bone formation (PMID: 11586292).

Synonyms

CED, DPD1, LAP, TGF B1, TGF beta 1, tgf beta1, TGFB, TGFB1, TGFbeta, tgf-beta1

Publications

Species	Title
human	J Biol Chem; Transforming growth factor β (TGF-β)-induced up-regulation of TGF-β receptors at the cell surface amplifies the TGF-β response.; Authors - Dana Duan
human	PLoS Biol; Up-regulation of FOXD1 by YAP alleviates senescence and osteoarthritis.; Authors - Lina Fu
human	Protein Cell; Telomere-dependent and telomere-independent roles of RAP1 in regulating human stem cell homeostasis.; Authors - Xing Zhang
	Immunity; A Mutation in the Transcription Factor Foxp3 Drives T Helper 2 Effector Function in Regulatory T Cells.; Authors - Frédéric Van Gool
human	J Cell Biochem; microRNA-539 functions as a tumor suppressor in papillary thyroid carcinoma via the transforming growth factor β1/Smads signaling pathway by targeting secretory leukocyte protease inhibitor.; Authors - Cheng-Bi Xu
human	Cell Stem Cell; FOXO3-Engineered Human ESC-Derived Vascular Cells Promote Vascular Protection and Regeneration.; Authors - Pengze Yan