HumanKine® recombinant human TGF beta 1 protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

Typically ≤ 0.5 ng/mL EC50

Specific Activity

>1.00 x 10⁷ IU/mg

Species Reactivity

human,mouse,goat,pig,rat

Purity

>95%

Publications

146

Cat no : HZ-1011

Synonyms

CED, DPD1, LAP, TGF B1, TGF beta 1, tgf beta1, TGFB, TGFB1, TGFbeta, tgf-beta1

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Validation Data Gallery

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  Purity of recombinant TGF beta 1 was determined by SDS-polyacrylamide gel electrophoresis. The protein was resolved in an SDS-polyacrylamide gel in reducing and non-reducing conditions followed by staining with Comassie blue.

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  Recombinant human TGF beta 1 (HZ-1011) inhibits IL-4 induced proliferation of the HT-2 mouse cell line. HT-2 cells are Balb/c spleen cells activated by sheep erythrocytes in the presence of IL-2. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-2 cells were treated with increasing concentrations of recombinant TGF beta 1 for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range is 0.01-0.17 ng/mL.

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Technical Specifications

• GeneID: 7040
• Species: Human
• Expression: HEK293
• Activity(Primary): Typically ≤ 0.5 ng/mL EC50
• Specific Activity: >1.00 x 10^7 IU/mg
• Purity: >95%
• Endotoxin: <1 EU/µg
• Accession Number: P01137
• Molecular Mass: 25 kDa, homodimer, nonglycosylated
• Formulation: 50 mM NaOAc pH 3.7, See Certificate of Analysis for details
• Species Reactivity: human,mouse,goat,pig,rat

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 4 mM HCl containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Whitepapers

Effective Polarization of Human Th17 Cells with Biologicaly Relevant HumanKine® TGFβ1, TGFβ2, and TGFβ3 Expressed in Human Cells

Effective Differentiation of Th17 Cells With HumanKine® Cytokines Expressed in Human Cells

Efficient Induction of Th17 Cells with HumanKine® TGFβ1

Background

TGFB, also named as LAP and TGFB1, is a multifunctional peptide that controls proliferation, and cell differentiation. TGF acts synergistically with TGFA in inducing transformation process. It also acts as a negative autocrine growth factor. Dysregulation of TGFB activation and signaling may result in apoptosis. Many cells synthesize TGFB having specific receptors for it. TGFB positively and negatively regulates many other growth factors. It plays an important role in bone remodeling being a potent stimulator of an osteoblastic bone formation (PMID: 11586292).

Synonyms

CED, DPD1, LAP, TGF B1, TGF beta 1, tgf beta1, TGFB, TGFB1, TGFbeta, tgf-beta1

Publications

Species	Title
human	Nature; Paracrine signalling by cardiac calcitonin controls atrial fibrogenesis and arrhythmia.; Authors - Lucia M Moreira
human	Nat Biotechnol; Multiple targets of miR-302 and miR-372 promote reprogramming of human fibroblasts to induced pluripotent stem cells.; Authors - DeepaSubramanyam
human	Nat Cell Biol; A20 promotes metastasis of aggressive basal-like breast cancers through multi-monoubiquitylation of Snail1.; Authors - Ji-Hyung Lee
mouse	Nat Commun; Smad6 inhibits non-canonical TGF-β1 signalling by recruiting the deubiquitinase A20 to TRAF6.; Authors - Su MyungJung
human	Nat Commun; Modelling Fanconi anemia pathogenesis and therapeutics using integration-free patient-derived iPSCs.; Authors - Guang-HuiLiu
human	Nat Commun; PTEN deficiency reprogrammes human neural stem cells towards a glioblastoma stem cell-like phenotype.; Authors - ShunleiDuan