• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human EGF protein


0.1-0.6 ng/mL

Specific Activity

minimally 3.00x106 IU/mg

Species Reactivity



> 95%

Cat no : HZ-1326



Technical Specifications

GeneID 1950
Species Human
Expression HEK293
Activity(Primary) 0.1-0.6 ng/mL
Specific Activityminimally 3.00x106 IU/mg
Purity > 95%
Endotoxin<1.0 EU/µg
Accession NumberP01133
Molecular Mass 6 kDa reduced, 10 kDa non-reduced, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse,rat

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.


EGF (epidermal growth factor) is a 6 kDA, single chain protein that serves a mitogenic factor for numerous cell types, particularly epithelial cells and fibroblasts. It is predominantly produced and secreted by the kidneys in humans. EGF activates cells through binding with its corresponding receptor (EGFR). Receptor binding activates several downstream pathways leading to cell proliferation, differentiation, and apoptosis. EGF plays important roles in embryonic development, tissue regeneration, transport through ion channels, and wound healing. Dysregulated EGF signaling has been linked with renal disease progression, and EGF levels could potentially be used as biomarker to monitor disease-related cell damage (PMID: 15110798, 24513230, 27868030).





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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


K. (Verified Customer) (10-26-2023)

Used it in 100-500ng concentration for 10-30 minutes to check EGFR activation and always worked well.

  • Applications: Western Blot,Immunofluorescence,Cell culture
  • Primary Antibody Dilution: 200ng
  • Cell Tissue Type: Human Liver cells