• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human FGFbasic-TS protein

Activity(Primary)

0.4-2.5 ng/mL EC50 in NIH3T3 cells in defined Media.

Specific Activity

>1.00 x 107 IU/mg

Species Reactivity

human,mouse,rat

Purity

>95%

Cat no : HZ-1285

Synonyms

Basic fibroblast growth factor, BFGF, FGF basic, FGF basic-TS, FGF2, FGFB, FGFbasic, FGFbasic-TS, HBGF 2



Technical Specifications

GeneID 2247
Species Human
Expression HEK293
Activity(Primary) 0.4-2.5 ng/mL EC50 in NIH3T3 cells in defined Media.
Activity(Secondary)0.05-0.4 ng/mL EC50 in HDFa cells
Specific Activity>1.00 x 107 IU/mg
Purity >95%
Endotoxin<1 EU/µg
Accession NumberP09038
Molecular Mass 17 kDa, monomer, non-glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse,rat

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

Basic Fibroblast growth factor (FGFbasic), also known as bFGF, FGF2, FGF-β or HBGF-2, belongs to the FGF family. FGFbasic is involved in a number of biological processes including embryonic development and differentiation, neuron differentiation, survival, and regeneration, and the proliferation of cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin (PMID 3272178, 3316527). FGFbasic is critical for human embryonic stem cells to remain in an undifferentiated state during cell culture (PMID 15782187).

Synonyms

Basic fibroblast growth factor, BFGF, FGF basic, FGF basic-TS, FGF2, FGFB, FGFbasic, FGFbasic-TS, HBGF 2

Publications

SpeciesTitle
human

Nat Biotechnol

An in vivo model of functional and vascularized human brain organoids.

Authors - Abed AlFatah Mansour
human

Cell Stem Cell

A Human Skeletal Muscle Atlas Identifies the Trajectories of Stem and Progenitor Cells across Development and from Human Pluripotent Stem Cells.

Authors - Haibin Xi
human

Nat Commun

KMT2A associates with PHF5A-PHF14-HMG20A-RAI1 subcomplex in pancreatic cancer stem cells and epigenetically regulates their characteristics

Authors - Mai Abdel Mouti
human

Cell Res

Visualization of aging-associated chromatin alterations with an engineered TALE system.

Authors - Ruotong Ren
mouse

Sci Adv

Varying mechanical forces drive sensory epithelium formation

Authors - Mingyu Xia
mouse

Sci Adv

ZMYND8 is a master regulator of 27-hydroxycholesterol that promotes tumorigenicity of breast cancer stem cells.

Authors - Maowu Luo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Ryszard (Verified Customer) (08-01-2022)

I tried Your HumanKine FGFbasic-TS in my culture and my cells are very happy. It's an excellent alternative to other FGFs from other companies that have trouble delivering on time their orders.

  • Applications: Cell culture
  • Cell Tissue Type: Neural Stem Cell Cultures
FH

Alessandro (Verified Customer) (07-27-2022)

Reliable supplement for dopaminergic neurons maturation

  • Applications: Cell culture
  • Cell Tissue Type: Dopaminergic Neurons
FH

Ana (Verified Customer) (03-16-2022)

Works well

  • Applications: Cell culture