- HEK293 expressed
- Endotoxin-free
- Animal-component free
HumanKine® recombinant human FGFbasic-TS protein
Activity(Primary)
0.4-2.5 ng/mL EC50 in NIH3T3 cells in defined Media.
Specific Activity
>1.00 x 107 IU/mg
Species Reactivity
human,mouse,rat
Purity
>95%
Cat no : HZ-1285
Synonyms
Basic fibroblast growth factor, BFGF, FGF basic, FGF basic-TS, FGF2, FGFB, FGFbasic, FGFbasic-TS, HBGF 2
Validation Data Gallery
Technical Specifications
GeneID | 2247 |
Species | Human |
Expression | HEK293 |
Activity(Primary) | 0.4-2.5 ng/mL EC50 in NIH3T3 cells in defined Media. |
Activity(Secondary) | 0.05-0.4 ng/mL EC50 in HDFa cells |
Specific Activity | >1.00 x 107 IU/mg |
Purity | >95% |
Endotoxin | <1 EU/µg |
Accession Number | P09038 |
Molecular Mass | 17 kDa, monomer, non-glycosylated |
Formulation | 1x PBS, See Certificate of Analysis for details |
Species Reactivity | human,mouse,rat |
Stability and Reconstitution
Stability and Storage | Product Form | Temperature Conditions | Storage Time (From Date of Receipt) |
---|---|---|---|
Lyophilized | -20°C to -80°C | Until Expiry Date | |
Lyophilized | Room Temperature | 2 weeks | |
Reconstituted as per CofA | -20°C to -80°C | 6 months | |
Reconstituted as per CofA | 4°C | 1 week | |
Avoid repeated freeze-thaw cycles. |
Reconstitution | Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). |
Background
Basic Fibroblast growth factor (FGFbasic), also known as bFGF, FGF2, FGF-β or HBGF-2, belongs to the FGF family. FGFbasic is involved in a number of biological processes including embryonic development and differentiation, neuron differentiation, survival, and regeneration, and the proliferation of cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin (PMID 3272178, 3316527). FGFbasic is critical for human embryonic stem cells to remain in an undifferentiated state during cell culture (PMID 15782187).
Synonyms
Basic fibroblast growth factor, BFGF, FGF basic, FGF basic-TS, FGF2, FGFB, FGFbasic, FGFbasic-TS, HBGF 2
Publications
Species | Title |
---|---|
Cell Stem Cell A Human Skeletal Muscle Atlas Identifies the Trajectories of Stem and Progenitor Cells across Development and from Human Pluripotent Stem Cells. | |
Cell Res Visualization of aging-associated chromatin alterations with an engineered TALE system. | |
Mol Psychiatry Sensing serotonin secreted from human serotonergic neurons using aptamer-modified nanopipettes. | |
J Clin Invest Mature myelin maintenance requires Qki to coactivate PPARβ-RXRα-mediated lipid metabolism. | |
Nat Commun Qki activates Srebp2-mediated cholesterol biosynthesis for maintenance of eye lens transparency. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Ryszard (Verified Customer) (08-01-2022) | I tried Your HumanKine FGFbasic-TS in my culture and my cells are very happy. It's an excellent alternative to other FGFs from other companies that have trouble delivering on time their orders.
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FH Alessandro (Verified Customer) (07-27-2022) | Reliable supplement for dopaminergic neurons maturation
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FH Ana (Verified Customer) (03-16-2022) | Works well
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