TUBB3-specific Monoclonal antibody

TUBB3-specific Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, rabbit, chicken, pig

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

1F8G10

Cat no : 66375-1-Ig

Synonyms

beta 4, beta tubulin, MC1R, TUBB, TUBB3, TUBB3-specific, Tubulin beta 3 chain, Tubulin beta III, tubulin, beta 3, Tuj1



Tested Applications

Positive WB detected inSH-SY5Y cells, PC-12 cells, Neuro-2a cells, Pig brain, Rabbit brain, Rat brain Mouse brain, Chicken brain, human brain tissue
Positive IHC detected inhuman cerebellum tissue, mouse brain tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:20000
Sample-dependent, check data in validation data gallery

Product Information

66375-1-Ig targets TUBB3-specific in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, rabbit, chicken, pig samples.

Tested Reactivity human, mouse, rat, rabbit, chicken, pig
Cited Reactivityhuman, mouse, rat, chicken
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name tubulin, beta 3
Calculated molecular weight 55 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberNM_001197181
Gene symbol TUBB3
Gene ID (NCBI) 10381
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TUBB3, the class III β tubulin or Tuj1, is selectively expressed in testis and neurons of the central and peripheral nervous system. It has been widely used as a marker for neurons. Aberrant expression of TUBB3 has also been found in various tumors of non-neural origin and can be used as a biomarker for cancer aggressiveness and a marker for the tendency to respond poorly to chemotherapy. This antibody is specific to TUBB3 but not cross-react with other tubulin isoforms.

Protocols

Product Specific Protocols
WB protocol for TUBB3-specific antibody 66375-1-IgDownload protocol
IHC protocol for TUBB3-specific antibody 66375-1-IgDownload protocol
IF protocol for TUBB3-specific antibody 66375-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Neuron

Posterior Neocortex-Specific Regulation of Neuronal Migration by CEP85L Identifies Maternal Centriole-Dependent Activation of CDK5.

Authors - Andrew Kodani
ratIF

Curr Biol

Pyramidal neuron morphogenesis requires a septin network that stabilizes filopodia and suppresses lamellipodia during neurite initiation

Authors - Megan R Radler
humanIF

Theranostics

Chlorogenic acid effectively treats cancers through induction of cancer cell differentiation.

Authors - Shuai Huang
mouseWB

Carbohydr Polym

Silk fibroin nanofibrous scaffolds incorporated with microRNA-222 loaded chitosan nanoparticles for enhanced neuronal differentiation of neural stem cells.

Authors - Zehao Li
humanIF

Elife

Single-nucleus transcriptomic analysis of human dorsal root ganglion neurons.

Authors - Minh Q Nguyen
mouseWB

J Neuroinflammation

Epigenetic suppression of liver X receptor β in anterior cingulate cortex by HDAC5 drives CFA-induced chronic inflammatory pain.

Authors - Yu-Jiao Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Gabriele (Verified Customer) (01-12-2023)

The antibody works very well with our IF protocol. Maybe the dilution could be pushed to 1:500-1:600. TUBB3 1:400 (green), Hoechst (Blue)

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:400
  • Cell Tissue Type: hiPSC-derived Neurons
TUBB3-specific Antibody Immunofluorescence validation (1:400 dilution) in hiPSC-derived Neurons (Cat no:66375-1-Ig)
FH

Alessandro (Verified Customer) (07-27-2022)

No aspecific staining, great outcome

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: ROs
FH

Josh (Verified Customer) (12-18-2018)

BIII tubulin antibody signal nicely fills neuronal cell bodies and processes with good signal to noise at a reasonable dilution (1:500 in 1% NDS, 0.3% Triton-X, PBS with donkey anti-mouse 647 secondary, 1:500). However, BIII tubulin antibody signal also colocalized with S100 immunoreactive astrocytic processes, though with a lesser intensity. Overall, the antibody is a useful marker of neuronal differentiation when used in combination with other cell-selective antibodies and morphology. Image caption: Differentiated adult hippocampal neural stem cell culture. BIII tubulin 1:500 (purple), S100 1:500 (red), Hoechst (blue). Scale bar = 20 um.

  • Applications: Immunofluorescence, Cell culture,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Adult hippocampal neural stem cells, differentiated for 7 days
TUBB3-specific Antibody Immunofluorescence,Cell culture, validation (1:500 dilution) in Adult hippocampal neural stem cells, differentiated for 7 days (Cat no:66375-1-Ig)