TUBB3-specific Monoclonal antibody
TUBB3-specific Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
human, mouse, rat, rabbit, chicken, pig
WB, IHC, IF, FC, ELISA
Cat no : 66375-1-Ig
Validation Data Gallery
|Positive WB detected in||SH-SY5Y cells, PC-12 cells, Neuro-2a cells, Pig brain, Rabbit brain, Rat brain Mouse brain, Chicken brain, human brain tissue|
|Positive IHC detected in||human cerebellum tissue, mouse brain tissue, mouse cerebellum tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||rat brain tissue, human neuron, iPS cells, mouse brain tissue|
|Positive FC detected in||SH-SY5Y cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:400-1:20000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|WB||See 10 publications below|
|IHC||See 2 publications below|
|IF||See 28 publications below|
|FC||See 2 publications below|
66375-1-Ig targets TUBB3-specific in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, rabbit, chicken, pig samples.
|Tested Reactivity||human, mouse, rat, rabbit, chicken, pig|
|Cited Reactivity||human, mouse, rat, chicken|
|Host / Isotype||Mouse / IgG1|
|Full Name||tubulin, beta 3|
|Calculated molecular weight||55 kDa|
|Observed molecular weight||50-55 kDa|
|GenBank accession number||NM_001197181|
|Gene ID (NCBI)||10381|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
TUBB3, the class III β tubulin or Tuj1, is selectively expressed in testis and neurons of the central and peripheral nervous system. It has been widely used as a marker for neurons. Aberrant expression of TUBB3 has also been found in various tumors of non-neural origin and can be used as a biomarker for cancer aggressiveness and a marker for the tendency to respond poorly to chemotherapy. This antibody is specific to TUBB3 but not cross-react with other tubulin isoforms.
|Product Specific Protocols|
|WB protocol for TUBB3-specific antibody 66375-1-Ig||Download protocol|
|IHC protocol for TUBB3-specific antibody 66375-1-Ig||Download protocol|
|IF protocol for TUBB3-specific antibody 66375-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
Posterior Neocortex-Specific Regulation of Neuronal Migration by CEP85L Identifies Maternal Centriole-Dependent Activation of CDK5.
Pyramidal neuron morphogenesis requires a septin network that stabilizes filopodia and suppresses lamellipodia during neurite initiation
Chlorogenic acid effectively treats cancers through induction of cancer cell differentiation.
Silk fibroin nanofibrous scaffolds incorporated with microRNA-222 loaded chitosan nanoparticles for enhanced neuronal differentiation of neural stem cells.
Single-nucleus transcriptomic analysis of human dorsal root ganglion neurons.
Epigenetic suppression of liver X receptor β in anterior cingulate cortex by HDAC5 drives CFA-induced chronic inflammatory pain.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Gabriele (Verified Customer) (01-12-2023)
The antibody works very well with our IF protocol. Maybe the dilution could be pushed to 1:500-1:600. TUBB3 1:400 (green), Hoechst (Blue)
Alessandro (Verified Customer) (07-27-2022)
No aspecific staining, great outcome
Josh (Verified Customer) (12-18-2018)
BIII tubulin antibody signal nicely fills neuronal cell bodies and processes with good signal to noise at a reasonable dilution (1:500 in 1% NDS, 0.3% Triton-X, PBS with donkey anti-mouse 647 secondary, 1:500). However, BIII tubulin antibody signal also colocalized with S100 immunoreactive astrocytic processes, though with a lesser intensity. Overall, the antibody is a useful marker of neuronal differentiation when used in combination with other cell-selective antibodies and morphology. Image caption: Differentiated adult hippocampal neural stem cell culture. BIII tubulin 1:500 (purple), S100 1:500 (red), Hoechst (blue). Scale bar = 20 um.