LHX6 Polyclonal antibody

• Featured Product
• KD/KO Validated

LHX6 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

Reviews (1)

Cat no : 21516-1-AP

Synonyms

LHX6, LHX6.1, LIM homeobox 6, LIM homeobox protein 6, LIM/homeobox protein Lhx6, LIM/homeobox protein Lhx6.1

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Validation Data Gallery

• 

  WB analysis of HepG2 using 21516-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21516-1-AP (LHX6 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

The immunogen of 21516-1-AP is LHX6 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LHX6 fusion protein Ag16047
• Full Name: LIM homeobox 6
• Calculated Molecular Weight: 392 aa, 43 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: BC103937
• Gene Symbol: LHX6
• Gene ID (NCBI): 26468
• RRID: AB_2878875
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LHX6 is a LIM homeodomain protein similar in structure to ISL-1. LHX6, like ISL-1, contains tandem LIM domains and a homeodomain that regulate its activity both in cis and through interaction with cell-specific factors. LHX6 is highly expressed in the neural crest-derived mesenchyme throughout odontogenesis and down-regulated after birth. However, LHX6 is also expressed in the palate epithelium, oral epithelium, and dental epithelium during craniofacial development. LHX6 regulates migration and specification of neuron subtypes and marks specific neurons. The molecular weight of Endogenous LHX6 is 40 kDa, but the LHX6
PITX2 protein complex is 56 kDa (PMID: 23229549).

Publications

Species	Application	Title
human	WB	Mol Ther Nucleic Acids; Placental trophoblast cell-derived exosomal microRNA-1290 promotes the interaction between endometrium and embryo by targeting LHX6.; Authors - Shuang Shi ; KD Validated
human	WB	Exp Cell Res; Hypoxia-induced exosomes promote hepatocellular carcinoma proliferation and metastasis via miR-1273f transfer.; Authors - You Yu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Reyes (Verified Customer) (07-29-2024)

LHX6 (in green) appears to work nicely marking some of my neurons (in red) in human brain FFPE tissue.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Brain Cortex

LHX6 Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:21516-1-AP)