TTF1; NKX2-1 Polyclonal antibody

TTF1; NKX2-1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 21777-1-AP

Synonyms

TTF1, NKX2 1, NK2 homeobox 1, NK 2, Homeobox protein Nkx-2.1

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Validation Data Gallery

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  WB analysis of HeLa using 21777-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21777-1-AP (NKX2-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21777-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21777-1-AP (NKX2-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 21777-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21777-1-AP (TTF1; NKX2-1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 21777-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 21777-1-AP (TTF1; NKX2-1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 21777-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21777-1-AP (TTF1; NKX2-1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 21777-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 21777-1-AP (TTF1; NKX2-1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, mouse lung tissue, rat brain tissue, A549 cells, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

21777-1-AP targets TTF1; NKX2-1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: NK2 homeobox 1
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: NM_003317
• Gene Symbol: TTF1
• Gene ID (NCBI): 7080
• RRID: AB_2878919
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

NKX2-1, also named as NKX2A, TITF1 and TTF1, belongs to the NK-2 homeobox family. It is a transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. NKX2-1 is crucial in the maintenance of the thyroid differentiation phenotype. It may play a role in lung development and surfactant homeostasis. Defects in NKX2-1 are the cause of benign hereditary chorea (BHC), choreoathetosis, hypothyroidism, and neonatal respiratory distress (CHNRD). The antibody is specific to NKX2-1.

Publications

Species	Application	Title
human	WB	Environ Toxicol Pharmacol; Role of estrogen receptors in thyroid toxicity induced by mono (2-ethylhexyl) phthalate via endoplasmic reticulum stress: An in vitro mechanistic investigation; Authors - Qi Xu