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PAX6 Polyclonal antibody
PAX6 Polyclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, FC, ELISA
Conjugate
Unconjugated
Cat no : 12323-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Y79 cells, mouse embryo tissue, human stomach tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue, mouse eye tissue, human retinoblastoma tissue, mouse embryo tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse brain tissue, iPS cells, Neuro-2a cells |
| Positive FC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 12 publications below |
| IHC | See 5 publications below |
| IF | See 34 publications below |
Product Information
12323-1-AP targets PAX6 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PAX6 fusion protein Ag2984 |
| Full Name | paired box 6 |
| Calculated molecular weight | 47 kDa |
| Observed molecular weight | 47 kDa |
| GenBank accession number | BC011953 |
| Gene symbol | PAX6 |
| Gene ID (NCBI) | 5080 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Background
Paired-box 6 (Pax6) is a highly conserved nuclear transcription factor that is key in neural tissue development, particularly eye morphogenesis.
What is the molecular weight of Pax6?
46.6 kDa. Pax6 is composed of 422 amino acids and contains a paired domain and a homeodomain.
What is the role of Pax6 in development?
In embryos, Pax6 is expressed in the developing central nervous system (CNS), eye, nose, and also in the pancreas. As a transcription factor, Pax6 is one of the earliest genes expressed in the eye and regulates the expression of many genes required for retinal and olfactory development (PMID: 10461206). The broad expression of Pax6 in the neuroectoderm, which develops to the CNS, makes it a useful marker for neural stem cells (PMID: 20621053).
The homology of Pax6 to the Drosophila gene eyeless and the mouse gene Small eye highlights its importance across a wide spectrum of organisms. In model organisms such as flies or mice, a dramatic phenotype can be seen. Mutations in eyeless lead to ectopic eye development and mice with heterozygote small eye mutations do not develop eyes or nasal cavities, and homozygote small eye mice do not survive (PMID: 7892602; PMID 8875254).
What is the role of Pax6 in eye disease?
Due to its key role as a master regulator in eye development, mutations in Pax6 have been associated with many different eye diseases. In humans, premature stop signals that lead to the production of a truncated protein have been found to cause aniridia, the absence of an iris (PMID: 19898691). Single amino acid mutations in Pax6 may lead to Peters' anomaly, which causes thinning and clouding of the cornea (PMID: 8162071).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PAX6 antibody 12323-1-AP | Download protocol |
| IHC protocol for PAX6 antibody 12323-1-AP | Download protocol |
| IF protocol for PAX6 antibody 12323-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Rep Astrocytes mediate cell non-autonomous correction of aberrant firing in human FXS neurons | ||
Cell Rep Evolutionary Gain of Dbx1 Expression Drives Subplate Identity in the Cerebral Cortex. | ||
Stem Cell Reports miR-342-5p Regulates Neural Stem Cell Proliferation and Differentiation Downstream to Notch Signaling in Mice. | ||
Cereb Cortex Dynamic Expression Patterns of Progenitor and Pyramidal Neuron Layer Markers in the Developing Human Hippocampus. | ||
Cereb Cortex Hippocampal Radial Glial Subtypes and Their Neurogenic Potential in Human Fetuses and Healthy and Alzheimer's Disease Adults. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Julie (Verified Customer) (01-18-2021) | This antibody works very well for immunofluorescence on human brain.
|
FH Rachel (Verified Customer) (02-01-2019) | Worked well and Proteintech employees were very helpful during the ordering process.
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