TBR1 Polyclonal antibody

TBR1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat


WB, IP, IF, IHC, ELISA, FC (Intra)



Cat no : 20932-1-AP


T box brain protein 1, T box, brain, 1, T brain 1, TBR 1, TBR1, TES 56

Tested Applications

Positive WB detected inmouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman brain tissue, mouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20932-1-AP is TBR1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TBR1 fusion protein Ag14935
Full Name T-box, brain, 1
Calculated Molecular Weight 682 aa, 74 kDa
Observed Molecular Weight 74 kDa
GenBank Accession NumberBC104844
Gene Symbol TBR1
Gene ID (NCBI) 10716
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBR1, also named as T-box brain protein 1, is a 682 amino acid protein, which contains one T-box DNA-binding domain and localizes in the nucleus. TBR1 is expressed in the brain and as a transcriptional regulator is involved in developmental processes. TBR1 is required for normal brain development.


Product Specific Protocols
WB protocol for TBR1 antibody 20932-1-APDownload protocol
IHC protocol for TBR1 antibody 20932-1-APDownload protocol
IF protocol for TBR1 antibody 20932-1-APDownload protocol
IP protocol for TBR1 antibody 20932-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Neurosci

A tau homeostasis signature is linked with the cellular and regional vulnerability of excitatory neurons to tau pathology.

Authors - Hongjun Fu

Nat Commun

Pathogenic POGZ mutation causes impaired cortical development and reversible autism-like phenotypes.

Authors - Kensuke Matsumura

Nat Commun

Disrupted neuronal maturation in Angelman syndrome-derived induced pluripotent stem cells.

Authors - James J Fink

Mol Neurodegener

AIF3 splicing switch triggers neurodegeneration.

Authors - Shuiqiao Liu

Proc Natl Acad Sci U S A

Human intermediate progenitor diversity during cortical development.

Authors - Mark-Phillip Pebworth

Cereb Cortex

N-Methyl d-Aspartate Receptor Expression Patterns in the Human Fetal Cerebral Cortex.

Authors - Inseyah Bagasrawala


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Reyes (Verified Customer) (03-01-2024)

Used on human brain cortical sections, it marked nicely the nucleus of my neurons.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Human Brain Cortex
TBR1 Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:20932-1-AP)

Mandi (Verified Customer) (03-09-2020)

Good staining on iPSC-derived Cortical neurons. Needed a decent amount of optimization to prevent non-specific binding though. Make sure to run primary/secondary deletes.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: human cortical neurons