N-cadherin Polyclonal antibody

• Featured Product
• KD/KO Validated

N-cadherin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA, Cell treatment

Conjugate

Unconjugated

Publications

1224

Reviews (8)

Cat no : 22018-1-AP

Synonyms

N cadherin, CDHN, CDH2, CD325, Cadherin-2

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Validation Data Gallery

• 

  WB analysis of HEK-293 using 22018-1-AP

  WB result of N-cadherin antibody (22018-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-N-cadherin transfected HEK-293 cells.

• 

  WB analysis using 22018-1-AP

  C2C12 (N-cadherin+), HeLa (N-cadherin+), MCF-7 (N-cadherin-), A431 (N-cadherin-) cell lysates were subjected to SDS PAGE followed by western blot with 22018-1-AP (N-cadherin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22018-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22018-1-AP (N-cadherin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22018-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22018-1-AP (N-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22018-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22018-1-AP (N-cadherin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22018-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22018-1-AP (N-cadherin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22018-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22018-1-AP (N-cadherin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 22018-1-AP

  IP result of anti-N-cadherin (IP:22018-1-AP, 3ug; Detection:22018-1-AP 1:1500) with mouse brain tissue lysate 7000ug.

• 

  IHC staining of mouse heart using 22018-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22018-1-AP (N-cadherin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 22018-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22018-1-AP (N-cadherin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 22018-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22018-1-AP (N-cadherin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 22018-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22018-1-AP (N-cadherin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 22018-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using N-cadherin antibody (22018-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 22018-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using N-cadherin antibody (22018-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of C2C12 using 22018-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using N-cadherin antibody (22018-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of C2C12 using 22018-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using N-cadherin antibody (22018-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), 594phalloidin (red).

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Tested Applications

• Positive WB detected in: mouse brain tissue, A549 cells, C2C12 cells, HEK-293 cells, rat brain tissue, HepG2 cells, NCI-H1299 cells, HeLa cells, C6 cells, PC-3 cells, rat heart tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse heart tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse heart tissue, C2C12 cells
• Positive IF/ICC detected in: C2C12 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 1148 publications below
• IHC: See 155 publications below
• IF: See 134 publications below
• FC: See 3 publications below
• CoIP: See 2 publications below

Product Information

22018-1-AP targets N-cadherin in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, canine, bovine, hamster, horse, gecko
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: N-cadherin fusion protein Ag16792
• Full Name: cadherin 2, type 1, N-cadherin (neuronal)
• Calculated Molecular Weight: 906 aa, 100 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC036470
• Gene Symbol: N-cadherin
• Gene ID (NCBI): 1000
• RRID: AB_2813891
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neuronal cadherin (N-cadherin), also known as cadherin-2 (CDH2), is a calcium-binding protein that mediates cell-cell adhesions of neuronal and some non-neuronal cell types. 

What is the molecular weight of N-cadherin? Is N-cadherin post-translationally modified?

The molecular weight of mature N-cadherin is 127 kDa. N-cadherin is synthesized in a precursor form that undergoes proteolytic cleavage by furin at the Golgi apparatus. Additionally, it can be phosphorylated by casein kinase II and N-glycosylated, which affects its stability (PMID: 12604612 and 19846557). 

What is the subcellular localization of N-cadherin? What is the tissue expression pattern of N-cadherin?

N-cadherin is an integral membrane protein present at the plasma membrane, forming adherens junctions. It is widely expressed in the nervous system, where it flanks the active zone of synapses and is important for synapse formation and remodeling. It is also present in the lens, skeletal, and cardiac muscles (PMID: 3857614). In the muscle, N-cadherin plays a role in myoblast differentiation, while in the heart it is required for the formation of intercalated discs. Additionally, N-cadherin is present in blood vessels, promoting angiogenesis by forming adhesive complexes between endothelial cells and pericytes (PMID: 24521477). 

What is the role of N-cadherin during the epithelial-mesenchymal transition (EMT)?

EMT is a crucial process during gastrulation that leads to the formation of mesenchymal cells. It is marked by decreased expression of E-cadherin and upregulation of N-cadherin, which promotes cell migration (PMID: 23481201). Similarly, upregulation of N-cadherin is observed in many cancer cell types and is associated with increased invasiveness and metastasis.

Publications

Species	Application	Title
human,mouse	WB,IHC	Mol Cancer; lncRNA ZNRD1-AS1 promotes malignant lung cell proliferation, migration, and angiogenesis via the miR-942/TNS1 axis and is positively regulated by the m6A reader YTHDC2; Authors - Jin Wang
mouse	WB	ACS Nano; Cancer-Erythrocyte Hybrid Membrane-Camouflaged Magnetic Nanoparticles with Enhanced Photothermal-Immunotherapy for Ovarian Cancer.; Authors - Jiaqiang Xiong
mouse	WB	ACS Nano; Biomimetic Nanomedicine Targeting Orchestrated Metabolism Coupled with Regulatory Factors to Disrupt the Metabolic Plasticity of Breast Cancer; Authors - Lingtong Meng
human	IHC	Nat Commun; Schwann cells regulate tumor cells and cancer-associated fibroblasts in the pancreatic ductal adenocarcinoma microenvironment; Authors - Meilin Xue
human	WB	Mol Cancer; CircGPR137B/miR-4739/FTO feedback loop suppresses tumorigenesis and metastasis of hepatocellular carcinoma.; Authors - Lianyong Liu
		Small; Temperature-Sensitive Polymer-Driven Nanomotors for Enhanced Tumor Penetration and Photothermal Therapy; Authors - Beng Ma

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

CHI (Verified Customer) (11-25-2024)

The antibody works great for IF

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: iPSC-induced cardiomyocytes

N-cadherin Antibody Immunohistochemistry validation (1:200 dilution) in iPSC-induced cardiomyocytes (Cat no:22018-1-AP)

FH

Greta (Verified Customer) (02-08-2024)

Good antibody for WB

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Rat cardiac fibroblasts

FH

Sarah (Verified Customer) (01-04-2024)

Signal at 1:500 was very weak and grainy by immunofluorescence of fixed HCT116 cells.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HCT116 cells.

FH

Sarah (Verified Customer) (02-09-2023)

Worked well for western blot of mouse brain for 1hr at room temp

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Mouse brain

FH

Ralph (Verified Customer) (05-17-2022)

The antibody works well in indirect immunofluorescence, stains the cell membrane.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: NIH 3T3

FH

Saba (Verified Customer) (02-21-2022)

The band intensity of the antibody is so sharp even in very diluted concentration.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000

FH

Lianjie (Verified Customer) (07-26-2019)

Works very well.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Mouse embryonic heart

N-cadherin Antibody Immunofluorescence, validation (1:200 dilution) in Mouse embryonic heart (Cat no:22018-1-AP)

FH

Aurelie (Verified Customer) (06-13-2019)

Great antibody, no background, works also in human U2OS and primary fibroblasts with the same efficiency.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Mouse Cortex/human cell lines

N-cadherin Antibody Western Blot, validation (1/1000 dilution) in Mouse Cortex/human cell lines (Cat no:22018-1-AP)

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