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N-cadherin Polyclonal antibody

N-cadherin Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (5)


WB, IP, IHC, IF-P, CoIP, ELISA, Cell treatment



Cat no : 22018-1-AP


Cadherin 2, CD325, CDH2, CDHN, CDw325, N cadherin, NCAD, N-cadherin, Neural cadherin

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue, HEK-293 cells, C2C12 cells, C6 cells, rat heart tissue, HeLa cells, PC-3 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse heart tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue, C2C12 cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22018-1-AP targets N-cadherin in WB, IP, IHC, IF-P, CoIP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, horse, hamster, canine, gecko, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen N-cadherin fusion protein Ag16792
Full Name cadherin 2, type 1, N-cadherin (neuronal)
Calculated Molecular Weight 906 aa, 100 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC036470
Gene Symbol N-cadherin
Gene ID (NCBI) 1000
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Neuronal cadherin (N-cadherin), also known as cadherin-2 (CDH2), is a calcium-binding protein that mediates cell-cell adhesions of neuronal and some non-neuronal cell types. 

What is the molecular weight of N-cadherin? Is N-cadherin post-translationally modified?

The molecular weight of mature N-cadherin is 127 kDa. N-cadherin is synthesized in a precursor form that undergoes proteolytic cleavage by furin at the Golgi apparatus. Additionally, it can be phosphorylated by casein kinase II and N-glycosylated, which affects its stability (PMID: 12604612 and 19846557). 

What is the subcellular localization of N-cadherin? What is the tissue expression pattern of N-cadherin?

N-cadherin is an integral membrane protein present at the plasma membrane, forming adherens junctions. It is widely expressed in the nervous system, where it flanks the active zone of synapses and is important for synapse formation and remodeling. It is also present in the lens, skeletal, and cardiac muscles (PMID: 3857614). In the muscle, N-cadherin plays a role in myoblast differentiation, while in the heart it is required for the formation of intercalated discs. Additionally, N-cadherin is present in blood vessels, promoting angiogenesis by forming adhesive complexes between endothelial cells and pericytes (PMID: 24521477). 

What is the role of N-cadherin during the epithelial-mesenchymal transition (EMT)?

EMT is a crucial process during gastrulation that leads to the formation of mesenchymal cells. It is marked by decreased expression of E-cadherin and upregulation of N-cadherin, which promotes cell migration (PMID: 23481201). Similarly, upregulation of N-cadherin is observed in many cancer cell types and is associated with increased invasiveness and metastasis.


Product Specific Protocols
WB protocol for N-cadherin antibody 22018-1-APDownload protocol
IHC protocol for N-cadherin antibody 22018-1-APDownload protocol
IF protocol for N-cadherin antibody 22018-1-APDownload protocol
IP protocol for N-cadherin antibody 22018-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Greta (Verified Customer) (02-08-2024)

Good antibody for WB

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Rat cardiac fibroblasts

Sarah (Verified Customer) (01-04-2024)

Signal at 1:500 was very weak and grainy by immunofluorescence of fixed HCT116 cells.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: HCT116 cells.

Sarah (Verified Customer) (02-09-2023)

Worked well for western blot of mouse brain for 1hr at room temp

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: Mouse brain

Ralph (Verified Customer) (05-17-2022)

The antibody works well in indirect immunofluorescence, stains the cell membrane.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: NIH 3T3

Saba (Verified Customer) (02-21-2022)

The band intensity of the antibody is so sharp even in very diluted concentration.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000

Lianjie (Verified Customer) (07-26-2019)

Works very well.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Mouse embryonic heart
N-cadherin Antibody Immunofluorescence, validation (1:200 dilution) in Mouse embryonic heart (Cat no:22018-1-AP)

Aurelie (Verified Customer) (06-13-2019)

Great antibody, no background, works also in human U2OS and primary fibroblasts with the same efficiency.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: Mouse Cortex/human cell lines
N-cadherin Antibody Western Blot, validation (1/1000 dilution) in Mouse Cortex/human cell lines (Cat no:22018-1-AP)