RELN Polyclonal antibody

RELN Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (1)





Cat no : 20689-1-AP


PRO1598, reelin, RELN, RL

Tested Applications

Positive IHC detected inhuman liver cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue

Recommended dilution

Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

20689-1-AP targets RELN in IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitypig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name reelin
Calculated Molecular Weight 388 kDa
GenBank Accession NumberNM_005045
Gene Symbol RELN
Gene ID (NCBI) 5649
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RELN belongs to the reelin family. It is an extracellular matrix serine protease that plays a role in layering of neurons in the cerebral cortex and cerebellum. It is a secreted glycoprotein which is essential for neuronal migration in the cortex. RELN regulates microtubule function in neurons and neuronal migration. It affects migration of sympathetic preganglionic neurons in the spinal cord, where it seems to act as a barrier to neuronal migration. Enzymatic activity is important for the modulation of cell adhesion. Binding to the extracellular domains of lipoprotein receptors VLDLR and ApoER2, RELN induces tyrosine phosphorylation of Dab1 and modulation of Tau phosphorylation. Defects in RELN are the cause of lissencephaly type 2 (LIS2).


Product Specific Protocols
IHC protocol for RELN antibody 20689-1-APDownload protocol
IF protocol for RELN antibody 20689-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Sci Bull (Beijing)

Single-cell dynamics of liver development in postnatal pigs

Authors - Lin Rao


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Reyes (Verified Customer) (03-01-2024)

I tried RELN (in red) in human paraffin brain sections and the result was a very good specificity.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Human Brain Cortex
RELN Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:20689-1-AP)

Etienne (Verified Customer) (12-19-2022)

Worked very well at 1/50 and 1/200

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1/50, 1/200, 1/500
  • Cell Tissue Type: Human Brain Organoid
RELN Antibody Immunofluorescence validation (1/50, 1/200, 1/500 dilution) in Human Brain Organoid (Cat no:20689-1-AP)