Why GFP-Trap gives the best immunoprecipitation results
Why GFP-Trap gives the best immunoprecipitation (IP) results
Affinity beads for immunoprecipitation (IP)
GFP-Trap is part of our Nano-Traps product line. They are ideal for fast and efficient immunoprecipitations (IP). Nano-Traps consist of VHHs, also termed Nanobodies, coupled to beads.
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Immunoprecipitation of GFP with GFP-Trap Agarose beads. I: Input, FT: Flow-Through, B: Bound
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Nano-Traps for IP:
- No heavy & light antibody chains
- Stringent washing conditions
- Low host cell protein background
- Very efficient pulldown, no protein in flow through (FT)
- High affinity to bind even proteins expressed at low levels
- Short incubation (30-60 min)
- Recombinantly expressed and validated
Applications:
- Immunoprecipitation/ Co-IP
- Mass spectrometry
- On-bead enzyme assays
- ChIP, RIP analysis
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| Product |
Formats (Product citations in parentheses) |
| GFP-Trap® |
Agarose (3,124), Magnetic Agarose (891), Magnetic Particle M-270 (24), Multiwell Plate (14) |
GST-Trap
|
Agarose (13) |
Halo-Trap
|
Agarose |
MBP-Trap
|
Agarose (5) |
Mdm4/HdmX-Trap
|
Agarose |
| MK2-Trap |
Agarose |
| mNeonGreen-Trap |
Agarose (6), Magnetic Agarose (10) |
| Myc-Trap® |
Agarose (98), Magnetic Agarose (50) |
| p53-N-term-Trap |
Agarose (5) |
| p53-C-term-Trap |
Agarose (1) |
| PARP1-Trap |
Agarose (5) |
| RFP-Trap® |
Agarose (214), Magnetic Agarose (72), Magnetic Particle M-270 |
| SNAP/CLIP-tag®-Trap |
Agarose (1) |
| Spot-Trap® |
Agarose (1), Magnetic Agarose, Magnetic Particle M-270 |
| TurboGFP-Trap |
Agarose (2), Magnetic Agarose |
| V5-Trap™ |
Agarose, Magnetic Agarose, Magnetic Particles M-270 |
Kits including lysis, wash, and elution buffers are available for all Nano-Trap products.