ChromoTek PARP1-Trap Agarose beads

PARP1-Trap Agarose is an affinity reagent for IP of human PARP1. It consists of a PARP1 Nanobody/ VHH, which is coupled to agarose beads.

Specificity

PARP1

Reactivity

Human

Applications

IP, CoIP

Conjugate

Agarose

Publications

6

Cat no : xta

Synonyms

PARP1-Trap, ADPRT, ADPRT 1, ADPRT1, pADPRT 1, PARP, PARP 1, PARP1, poly (ADP ribose) polymerase 1, Poly [ADP ribose] polymerase 1, Poly[ADP ribose] synthase 1, PPOL

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Validation Data Gallery

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  Immunoprecipitation of PARP1 with PARP1-Trap.I: Input, FT: Flow-through, B: Bound.

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Product Information

PARP1-Trap Agarose is an affinity reagent for IP of human PARP1. It consists of a PARP1 Nanobody/ VHH, which is coupled to agarose beads.

• Description: Immunoprecipitation of PARP1 with PARP1-Trap Agarose.

  • Effectively immunoprecipitates PARP1 (human)

  • Functional after IP: on-bead parylation assay

  • No heavy & light chains in your downstream application

• Applications: IP, CoIP
• Specificity/Target: PARP1, Epitope: Within DNA binding domain of PARP1; does not bind to PARP2, 3, and 9
• Tested Reactivity: Human
• Conjugate: Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
• Elution buffer: SDS sample buffer; 0.2 M glycine pH 2.5
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Compatibility with mass spectrometry: The PARP1-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
• RRID: AB_2631384
• Storage Buffer: 20% ethanol
• Storage Condition: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!

Other Formats

PARP1-Trap Agarose kit

Publications

Application	Title
IP	Cell; An HPF1/PARP1-Based Chemical Biology Strategy for Exploring ADP-Ribosylation.; Authors - Juan José Bonfiglio
IP	Mol Cell; Serine ADP-Ribosylation Depends on HPF1.; Authors - Juan José Bonfiglio
IP	EMBO J; Unanchored tri-NEDD8 inhibits PARP-1 to protect from oxidative stress-induced cell death.; Authors - Matthew J Keuss
IP	EMBO Rep; Ewing sarcoma protein promotes dissociation of poly(ADP-ribose) polymerase 1 from chromatin.; Authors - Seon-Gyeong Lee
IP	PLoS One; A New Nanobody-Based Biosensor to Study Endogenous PARP1 In Vitro and in Live Human Cells.; Authors - Andrea Buchfellner
ChIP	Exp Mol Med; Mitochondrial PARP1 regulates NAD(+)-dependent poly ADP-ribosylation of mitochondrial nucleoids; Authors - Jong-Hyuk Lee