Cat no : xta
PARP1-Trap Agarose is an affinity reagent for IP of human PARP1. It consists of a PARP1 Nanobody/ VHH, which is coupled to agarose beads.
|Immunoprecipitation of PARP1 with PARP1-Trap Agarose.
• Effectively immunoprecipitates PARP1 (human)
• Functional after IP: on-bead parylation assay
• No heavy & light chains in your downstream application
|PARP1, Epitope: Within DNA binding domain of PARP1
does not bind to PARP2, 3, and 9
|Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
|SDS sample buffer
0.2 M glycine pH 2.5
|Compatibility with mass spectrometry
|The PARP1-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
|Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!
An HPF1/PARP1-Based Chemical Biology Strategy for Exploring ADP-Ribosylation.
Serine ADP-Ribosylation Depends on HPF1.
Unanchored tri-NEDD8 inhibits PARP-1 to protect from oxidative stress-induced cell death.
Ewing sarcoma protein promotes dissociation of poly(ADP-ribose) polymerase 1 from chromatin.
A New Nanobody-Based Biosensor to Study Endogenous PARP1 In Vitro and in Live Human Cells.
Exp Mol Med
Mitochondrial PARP1 regulates NAD(+)-dependent poly ADP-ribosylation of mitochondrial nucleoids