ChromoTek PARP1-Trap Agarose beads

PARP1-Trap Agarose is an affinity reagent for IP of human PARP1. It consists of a PARP1 Nanobody/ VHH, which is coupled to agarose beads.

Specificity

PARP1

Reactivity

Human

Applications

IP, CoIP

Conjugate

Agarose

Cat no : xta

Synonyms

PARP1-Trap, ADPRT, ADPRT 1, ADPRT1, pADPRT 1, PARP, PARP 1, PARP1, poly (ADP ribose) polymerase 1, Poly [ADP ribose] polymerase 1, Poly[ADP ribose] synthase 1, PPOL



Product Information

PARP1-Trap Agarose is an affinity reagent for IP of human PARP1. It consists of a PARP1 Nanobody/ VHH, which is coupled to agarose beads.

DescriptionImmunoprecipitation of PARP1 with PARP1-Trap Agarose.

• Effectively immunoprecipitates PARP1 (human)

• Functional after IP: on-bead parylation assay

• No heavy & light chains in your downstream application

ApplicationsIP, CoIP
Specificity/TargetPARP1, Epitope: Within DNA binding domain of PARP1
does not bind to PARP2, 3, and 9
Tested ReactivityHuman
ConjugateAgarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5
Type Nanobody
ClassRecombinant
HostAlpaca
Compatibility with mass spectrometryThe PARP1-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2631384
Storage Buffer20% ethanol
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!

Publications

ApplicationTitle
IP

Cell

An HPF1/PARP1-Based Chemical Biology Strategy for Exploring ADP-Ribosylation.

Authors - Juan José Bonfiglio
IP

Mol Cell

Serine ADP-Ribosylation Depends on HPF1.

Authors - Juan José Bonfiglio
IP

EMBO J

Unanchored tri-NEDD8 inhibits PARP-1 to protect from oxidative stress-induced cell death.

Authors - Matthew J Keuss
IP

EMBO Rep

Ewing sarcoma protein promotes dissociation of poly(ADP-ribose) polymerase 1 from chromatin.

Authors - Seon-Gyeong Lee
IP

PLoS One

A New Nanobody-Based Biosensor to Study Endogenous PARP1 In Vitro and in Live Human Cells.

Authors - Andrea Buchfellner