ChromoTek Spot-Trap® Magnetic Agarose

Spot-Trap® Magnetic Agarose is an affinity reagent for immunoprecipitation (IP) of Spot-tagged proteins. It consists of an anti-Spot-Tag® VHH/ Nanobody that is coupled to magnetic agarose beads.

Specificity

Spot-Tag®

Applications

IP, CoIP, ChIP, RIP

Conjugate

Magnetic agarose

Type

Nanobody

Cat no : etma

Synonyms

Spot tag, Spot, PDRVRAVSHWSS, Spot-Trap



Product Information

Spot-Trap® Magnetic Agarose is an affinity reagent for immunoprecipitation (IP) of Spot-tagged proteins. It consists of an anti-Spot-Tag® VHH/ Nanobody that is coupled to magnetic agarose beads.

DescriptionImmunoprecipitation of Spot-tagged proteins and their interacting factors with anti-Spot Nanobody conjugated to Magnetic Agarose.

• Magnetic separation with easy and efficient washing of Spot-Trap Magnetic Agarose

• IP and Co-IP of Spot-tagged proteins without size limitation

• No contaminating heavy and light antibody chains

• Suitable for downstream mass spec analysis

• Recombinantly expressed without batch-to-batch variation

• Well-characterized and validated

• Ready-to-use

• Short incubation time of 30-60 minutes

ApplicationsIP, CoIP, ChIP, RIP
Specificity/TargetSpot-Tag® sequence PDRVRAVSHWSS
Binding capacity17.5 μg of recombinant Spot-tagged protein (~30 kDa) per 25 μL bead slurry
ConjugateMagnetic agarose beads; bead size: ~40 µm (cross-linked 6 % magnetic agarose beads)
Elution bufferSDS sample buffer
10 mM NaOH pH 12
Wash buffer compatibility2 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100, 0.1 % SDS
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) Dissociation constant KD of 6 nM
Compatibility with mass spectrometryThe Spot-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2827591
Storage Buffer20% ethanol
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!

Publications

ApplicationTitle
IP

Mol Cell

Caspase cleavage releases a nuclear protein fragment that stimulates phospholipid scrambling at the plasma membrane.

Authors - Masahiro Maruoka
IP

Nucleic Acids Res

Readthrough of stop codons under limiting ABCE1 concentration involves frameshifting and inhibits nonsense-mediated mRNA decay.

Authors - Giuditta Annibaldis
IP

Cells

The Phosphoarginine Phosphatase PtpB from Staphylococcus aureus Is Involved in Bacterial Stress Adaptation during Infection.

Authors - Mohamed Ibrahem Elhawy
IP

J Biol Chem

BioID screening of biotinylation sites using the avidin-like protein Tamavidin 2-REV identifies global interactors of stimulator of interferon genes (STING).

Authors - Kou Motani

Mol Carcinog

Detection of rapidly accumulating stress-induced SUMO in prostate cancer cells by a fluorescent SUMO biosensor.

Authors - Rui Yin
IP

Life Sci Alliance

The stabilized Pol31-Pol3 interface counteracts Pol32 ablation with differential effects on repair.

Authors - Kenji Shimada