Cat no : sta
GST-Trap is an affinity resin for IP of GST fusion proteins. It consists of a GST Nanobody/ VHH coupled to agarose beads.
|Description||Immunoprecipitation of GST-fusion proteins and their interacting factors with anti-GST Nanobody conjugated to beads.
• Effectively immunoprecipitates low expressed GST-fusion proteins
• Considerably higher affinity than glutathione cellulose and other affinity resins
• Strong binding even under harsh washing conditions
• No heavy & light chains in your downstream application
|Applications||IP, CoIP, ChIP, RIP|
|Specificity/Target||Glutathione S-Transferase (GST) from Schistosoma japonicum|
|Binding capacity||7.5-10 μg of recombinant GST per 25 μL bead slurry|
|Conjugate||Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)|
|Elution buffer||SDS sample buffer|
0.2 M glycine pH 2.5
|Wash buffer compatibility||4 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100|
|Affinity (KD)||Dissociation constant KD of 1 nM|
|Compatibility with mass spectrometry||The GST-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry|
|Storage Buffer||20% ethanol|
|Storage Condition||Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!|
|SDS GST-Trap Agarose (PDF)|
|Protocol GST-Trap Agarose (PDF)|
|Immunoprecipitation of GST- and MBP-fusion proteins (PDF)|
|Product brochure GST-Trap (PDF)|
|Troubleshooting guide immunoprecipitation (PDF)|
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