ChromoTek mNeonGreen-Trap Magnetic Agarose

The ChromoTek mNeonGreen-Trap Magnetic Agarose is an affinity resin for IP of mNeonGreen-fusion proteins. It consists of a mNeonGreen Nanobody/ VHH coupled to magnetic agarose beads.

Specificity

mNeonGreen

Applications

IP, CoIP, ChIP, RIP

Conjugate

Magnetic agarose

Type

Nanobody

Cat no : ntma

Synonyms

mNeonGreen-Trap, mNeonGreen, multimeric yellow fluorescence protein, mNeonGreen tag



Product Information

The ChromoTek mNeonGreen-Trap Magnetic Agarose is an affinity resin for IP of mNeonGreen-fusion proteins. It consists of a mNeonGreen Nanobody/ VHH coupled to magnetic agarose beads.

DescriptionImmunoprecipitation of mNeonGreen-fusion proteins and their interacting factors with anti-mNeonGreen Nanobody conjugated to magnetic agarose beads.

• Fast, reliable & efficient one-step immunoprecipitation

• Ready-to-use

• No heavy & light antibody chains

• Stable under harsh washing conditions

• Suitable for downstream mass spec analysis

• Works in samples from: mammals, plants, bacteria, yeast, insects etc.

ApplicationsIP, CoIP, ChIP, RIP
Specificity/TargetmNeonGreen
Binding capacity30 μg of recombinant mNeonGreen per 25 μL bead slurry
ConjugateMagnetic agarose beads; bead size: ~40 µm (cross-linked 6 % magnetic agarose beads)
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5
Wash buffer compatibility4 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100, 0.2 % SDS
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) Dissociation constant KD of 2 nM
Compatibility with mass spectrometryThe mNeonGreen-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2827594
Storage Buffer20% ethanol
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!

Publications

ApplicationTitle
IP

Mol Cell

Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex.

Authors - Michael L van de Weijer
CoIP

Nucleic Acids Res

The TH1 cell lineage-determining transcription factor T-bet suppresses TH2 gene expression by redistributing GATA3 away from TH2 genes.

Authors - Arnulf Hertweck
IP

Dev Cell

NLR-1/CASPR Anchors F-Actin to Promote Gap Junction Formation.

Authors - Lingfeng Meng
IP

EMBO J

Structural basis of TRAPPIII-mediated Rab1 activation.

Authors - Aaron Mn Joiner

Proc Natl Acad Sci U S A

PRD-2 mediates clock-regulated perinuclear localization of clock gene RNAs within the circadian cycle of Neurospora

Authors - Bradley M Bartholomai
IP

J Cell Biol

Factors promoting nuclear envelope assembly independent of the canonical ESCRT pathway.

Authors - I-Ju Lee