ChromoTek TurboGFP-Trap Agarose beads

TurboGFP-Trap Agarose is an affinity bead for immunoprecipitation (IP) of TurboGFP fusion proteins It comprises an anti-TurboGFP VHH/ Nanobody coupled to agarose beads.

Specificity

TurboGFP, CopGFP, maxGFP

Applications

IP, CoIP

Conjugate

Agarose

Type

Nanobody

Cat no : tbta

Synonyms

GFP-Trap, GFP-Trap Agarose, GFP-Trap Agarose beads, tbta, tbta-10, tbta-100, tbta-20, tbta-200, tbta-400, TurboGFP, TurboGFP tag, TurboGFP-tag, TurboGFP-Trap, TurboGFP-Trap Agarose beads



Product Information

TurboGFP-Trap Agarose is an affinity bead for immunoprecipitation (IP) of TurboGFP fusion proteins It comprises an anti-TurboGFP VHH/ Nanobody coupled to agarose beads.

DescriptionImmunoprecipitation of TurboGFP-fusion proteins and their interacting factors with anti-TurboGFP Nanobody conjugated to beads.

• Fast, reliable & efficient one-step immunoprecipitation

• Ready-to-use

• No heavy & light antibody chains

• Stable under harsh washing conditions

• Suitable for downstream mass spec analysis

• Works in samples from: mammals, plants, bacteria, yeast, insects etc.

ApplicationsIP, CoIP
Specificity/TargetTurboGFP, CopGFP, maxGFP, maxFP-Green
For the complete list, please click here: Fluorescent protein specificity table
Binding capacity7.5-10 μg of recombinant TurboGFP per 25 μL bead slurry
ConjugateAgarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
Elution bufferSDS sample buffer
0.1 mM citrate acid pH 3.0
Wash buffer compatibility6 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100, 0.2 % SDS
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) Dissociation constant KD of 0.5 nM
Compatibility with mass spectrometryThe TurboGFP-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2827596
Storage Buffer20% ethanol
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!

Publications

ApplicationTitle
IP

Nat Immunol

IRAP(+) endosomes restrict TLR9 activation and signaling.

Authors - Joel Babdor
IP

Nat Cell Biol

FERARI is required for Rab11-dependent endocytic recycling.

Authors - Jachen A Solinger
IP

Nucleic Acids Res

Induction of senescence upon loss of the Ash2l core subunit of H3K4 methyltransferase complexes.

Authors - Agnieszka Bochyńska
IP,WB

Brain

TRPA1 modulation by Sigma-1 receptor prevents oxaliplatin-induced painful peripheral neuropathy

Authors - Aida Marcotti
IP

Redox Biol

Alexander disease GFAP R239C mutant shows increased susceptibility to lipoxidation and elicits mitochondrial dysfunction and oxidative stress.

Authors - Álvaro Viedma-Poyatos
IP

Plant Cell

ERECTA regulates seed size independently of its intracellular domain via MAPK-DA1-UBP15 signaling.

Authors - Xuedan Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tobias (Verified Customer) (10-25-2022)

excellent efficiency and yield

  • Applications: Immunoprecipitation