Cat no : mbta
The ChromoTek MBP-Trap Agarose are affinity beads for IP of MBP-fusion proteins. They comprise MBP Nanobodies/ VHHs coupled to agarose beads.
|Immunoprecipitation of MBP-fusion proteins and their interacting factors with anti-MBP Nanobody conjugated to beads.
• Immunoprecipitation of low abundant MBP-fusion proteins
• Significantly higher sensitivity than amylose resin and other affinity resins
• No heavy & light antibody chains
• Stable under harsh washing conditions
• Suitable for downstream mass spec analysis
|IP, CoIP, ChIP, RIP
|Maltose-binding protein (MBP) of Escherichia coli
|45 μg (0.625 nmol) of recombinant MBP-tagged protein (~74.2 kDa) per 25 μL bead slurry
|Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
|SDS sample buffer
0.2 M glycine pH 2.5
|Wash buffer compatibility
|2 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100
|Dissociation constant KD of 4 nM
|Compatibility with mass spectrometry
|TheMBP-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
|Upon receipt store at +4°C. Do not freeze!
|SDS MBP-Trap Agarose (PDF)
|Protocol MBP-Trap Agarose (PDF)
|Troubleshooting guide immunoprecipitation (PDF)
|Immunoprecipitation of GST- and MBP-fusion proteins (PDF)
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