ChromoTek MBP-Trap Agarose beads

The ChromoTek MBP-Trap Agarose are affinity beads for IP of MBP-fusion proteins. They comprise MBP Nanobodies/ VHHs coupled to agarose beads.

Specificity

MBP

Applications

IP, CoIP, ChIP, RIP

Conjugate

Agarose

Type

Nanobody

Publications

19

Cat no : mbta

Synonyms

MBP, Maltose binding protein, MBP-Trap

---

Validation Data Gallery

• 

  Immunoprecipitation of maltose binding protein (MBP) from E. coli cell extract. The Western blot shows the very high effectivity of the MBP-Trap: No MBP is left in Flow-Through lane.I: Input, FT: Flow-Through, B: Bound.

---

Product Information

The ChromoTek MBP-Trap Agarose are affinity beads for IP of MBP-fusion proteins. They comprise MBP Nanobodies/ VHHs coupled to agarose beads.

• Description: Immunoprecipitation of MBP-fusion proteins and their interacting factors with anti-MBP Nanobody conjugated to beads.

  • Immunoprecipitation of low abundant MBP-fusion proteins

  • Significantly higher sensitivity than amylose resin and other affinity resins

  • No heavy & light antibody chains

  • Stable under harsh washing conditions

  • Suitable for downstream mass spec analysis

• Applications: IP, CoIP, ChIP, RIP
• Specificity/Target: Maltose-binding protein (MBP) of Escherichia coli
• Binding capacity: 45 μg (0.625 nmol) of recombinant MBP-tagged protein (~74.2 kDa) per 25 μL bead slurry
• Conjugate: Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
• Elution buffer: SDS sample buffer; 0.2 M glycine pH 2.5
• Wash buffer compatibility: 2 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity (KD): Dissociation constant K_D of 4 nM
• Compatibility with mass spectrometry: TheMBP-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
• RRID: AB_2631382
• Storage Buffer: 20% EtOH
• Storage Condition: Upon receipt store at +4°C. Do not freeze!

Other Formats

MBP-Trap Agarose kit

Publications

Application	Title
IP	Mol Cell; Centromere/kinetochore is assembled through CENP-C oligomerization; Authors - Masatoshi Hara
	Mol Cell; A PARylation-phosphorylation cascade promotes TOPBP1 loading and RPA-RAD51 exchange in homologous recombination.; Authors - Jiao Zhao
IP	Dev Cell; Importin-β Directly Regulates the Motor Activity and Turnover of a Kinesin-4.; Authors - Anindya Ganguly
IP	Plant Cell; FRA1 Kinesin Modulates the Lateral Stability of Cortical Microtubules through Cellulose Synthase-Microtubule Uncoupling Proteins.; Authors - Anindya Ganguly
IP	Plant Cell; TTL Proteins Scaffold Brassinosteroid Signaling Components at the Plasma Membrane to Optimize Signal Transduction in Arabidopsis.; Authors - Vítor Amorim-Silva
IP	Curr Biol; Arabidopsis pavement cell shape formation involves spatially confined ROPGAP regulators.; Authors - Theresa Lauster