ChromoTek mNeonGreen-Trap Agarose beads

mNeonGreen-Trap Agarose is an affinity reagent for IP of mNeonGreen-fusion proteins. It consists of a mNeonGreen Nanobody/ VHH coupled to agarose beads.

Specificity

mNeonGreen

Applications

IP, CoIP, ChIP, RIP

Type

Nanobody

Conjugate

Agarose

Cat no : nta

Synonyms

mNeonGreen-Trap, mNeonGreen, multimeric yellow fluorescence protein, mNeonGreen tag



Product Information

mNeonGreen-Trap Agarose is an affinity reagent for IP of mNeonGreen-fusion proteins. It consists of a mNeonGreen Nanobody/ VHH coupled to agarose beads.

DescriptionImmunoprecipitation of mNeonGreen-fusion proteins and their interacting factors with anti-mNeonGreen Nanobody conjugated to beads.

• Fast, reliable & efficient one-step immunoprecipitation

• Ready-to-use

• No heavy & light antibody chains

• Stable under harsh washing conditions

• Suitable for downstream mass spec analysis

• Short incubation time of about 60 minutes

ApplicationsIP, CoIP, ChIP, RIP
Specificity/TargetmNeonGreen
Binding capacity30 μg of recombinant mNeonGreen per 25 μL bead slurry
ConjugateAgarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5
Wash buffer compatibility4 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100, 0.2 % SDS
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) Dissociation constant KD of 2 nM
Compatibility with mass spectrometryThe mNeonGreen-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2827593
Storage Buffer20% EtOH
Storage ConditionUpon receipt store at +4°C. Do not freeze!

Publications

ApplicationTitle
CoIP

Dev Cell

The ER ladder is a unique morphological feature of developing mammalian axons.

Authors - Emiliano Zamponi
IP

Proc Natl Acad Sci U S A

The Bardet-Biedl syndrome protein complex is an adapter expanding the cargo range of intraflagellar transport trains for ciliary export.

Authors - Peiwei Liu
IP

J Cell Biol

SGEF forms a complex with Scribble and Dlg1 and regulates epithelial junctions and contractility.

Authors - Sahezeel Awadia
IP

J Cell Biol

The HSPG syndecan is a core organizer of cholinergic synapses.

Authors - Xin Zhou
WB

J Fungi (Basel)

Structure of the Yeast Cell Wall Integrity Sensor Wsc1 Reveals an Essential Role of Surface-Exposed Aromatic Clusters.

Authors - Philipp Schöppner
IP

Cell Stem Cell

Vimentin Coordinates Protein Turnover at the Aggresome during Neural Stem Cell Quiescence Exit.

Authors - Christopher S Morrow