Cat no : ota
Halo-Trap Agarose are affinity beads for IP of Halo-tag proteins. It comprises a Halo-tag Nanobody /VHH conjugated to agarose beads.
|Immunoprecipitation of Halo-tagged proteins and their interacting factors with anti-Halo Nanobody conjugated to beads.
• Halo-Trap immunoprecipitates Halo-fusion proteins even when already covalently bound to Halo-ligands, i.e. after labelling etc.
• Fast, reliable & efficient one-step immunoprecipitation
• No heavy & light antibody chains
• Bound Halo-fusion protein can be eluted without protease
• Suitable for downstream mass spec analysis
|IP, CoIP, ChIP, RIP
|Halo-tag (modified variant of the bacterial haloalkane dehalogenase enzyme from Rhodococcus rhodochrous) in the absence or presence of covalently bound chloralkane-based ligands.
|7.5-10 μg of recombinant Halo-tag per 25 μL bead slurry
|Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
|SDS sample buffer
0.2 M glycine pH 2.5
|Wash buffer compatibility
|4 M urea, 1 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100
|Dissociation constant KD of 2 nM
|Compatibility with mass spectrometry
|The Halo-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
|Upon receipt store at +4°C. Do not freeze!
|SDS Halo-Trap Agarose (PDF)
|Protocol Halo-Trap Agarose (PDF)
|Troubleshooting guide immunoprecipitation (IP) (PDF)
TMX4-driven LINC complex disassembly and asymmetric autophagy of the nuclear envelope upon acute ER stress
RNA methylation influences TDP43 binding and disease pathogenesis in models of amyotrophic lateral sclerosis and frontotemporal dementia
Poly(ADP-ribose) drives condensation of FUS via a transient interaction.
Nat Chem Biol
Bright and stable luminescent probes for target engagement profiling in live cells.
Galectin-9 restricts hepatitis B virus replication via p62/SQSTM1-mediated selective autophagy of viral core proteins.
ACS Chem Biol
Modulation of Phosphoprotein Activity by Phosphorylation Targeting Chimeras (PhosTACs).
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Jutta (Verified Customer) (08-23-2023)
very easy protocoll, fully successfull, low background, good signal on western blot
Anastasija (Verified Customer) (02-14-2022)
I used the Halo-trap agarose to perform nuclear co-IP of Halo-tagged proteins, and after a bit of optimization of the IP protocol I achieved very good pulldown efficiency with high amounts of bait and interactors, and low background. An added benefit is that upon elution there is no IgG co-eluting with the target proteins, which is normally a problem in conventional IP approaches using IgG. I am really happy with this product, and will continue using it in future experiments.