ChromoTek Halo-Trap Agarose beads

Halo-Trap Agarose are affinity beads for IP of Halo-tag proteins. It comprises a Halo-tag Nanobody /VHH conjugated to agarose beads.

Specificity

Halo-Tag

Applications

IP, CoIP, ChIP, RIP

Conjugate

Agarose

Type

Nanobody

Cat no : ota

Synonyms

Halo-Trap, Halo tag, dehalogenase



Product Information

Halo-Trap Agarose are affinity beads for IP of Halo-tag proteins. It comprises a Halo-tag Nanobody /VHH conjugated to agarose beads.

DescriptionImmunoprecipitation of Halo-tagged proteins and their interacting factors with anti-Halo Nanobody conjugated to beads.

• Halo-Trap immunoprecipitates Halo-fusion proteins even when already covalently bound to Halo-ligands, i.e. after labelling etc.

• Fast, reliable & efficient one-step immunoprecipitation

• No heavy & light antibody chains

• Bound Halo-fusion protein can be eluted without protease

• Suitable for downstream mass spec analysis

ApplicationsIP, CoIP, ChIP, RIP
Specificity/TargetHalo-tag (modified variant of the bacterial haloalkane dehalogenase enzyme from Rhodococcus rhodochrous) in the absence or presence of covalently bound chloralkane-based ligands.
Binding capacity7.5-10 μg of recombinant Halo-tag per 25 μL bead slurry
ConjugateAgarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5
Wash buffer compatibility4 M urea, 1 M NaCl, 10 mM DTT,  2 % Nonidet P40 Substitute, 1 % Triton X-100
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) Dissociation constant KD of 2 nM
Compatibility with mass spectrometryThe Halo-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2827595
Storage Buffer20% ethanol
Storage ConditionUpon receipt store at +4°C. Do not freeze!

Publications

ApplicationTitle
WB,IP

Mol Cell

Poly(ADP-ribose) drives condensation of FUS via a transient interaction.

Authors - Kevin Rhine

Nat Chem Biol

Bright and stable luminescent probes for target engagement profiling in live cells.

Authors - N Connor Payne
IP

Nat Commun

Galectin-9 restricts hepatitis B virus replication via p62/SQSTM1-mediated selective autophagy of viral core proteins.

Authors - Kei Miyakawa
IP

ACS Chem Biol

Modulation of Phosphoprotein Activity by Phosphorylation Targeting Chimeras (PhosTACs).

Authors - Po-Han Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Anastasija (Verified Customer) (02-14-2022)

I used the Halo-trap agarose to perform nuclear co-IP of Halo-tagged proteins, and after a bit of optimization of the IP protocol I achieved very good pulldown efficiency with high amounts of bait and interactors, and low background. An added benefit is that upon elution there is no IgG co-eluting with the target proteins, which is normally a problem in conventional IP approaches using IgG. I am really happy with this product, and will continue using it in future experiments.

  • Applications: Immunoprecipitation
  • Cell Tissue Type: HEK293T