Cat no : ota
Halo-Trap Agarose are affinity beads for IP of Halo-tag proteins. It comprises a Halo-tag Nanobody /VHH conjugated to agarose beads.
|Description||Immunoprecipitation of Halo-tagged proteins and their interacting factors with anti-Halo Nanobody conjugated to beads.
• Halo-Trap immunoprecipitates Halo-fusion proteins even when already covalently bound to Halo-ligands, i.e. after labelling etc.
• Fast, reliable & efficient one-step immunoprecipitation
• No heavy & light antibody chains
• Bound Halo-fusion protein can be eluted without protease
• Suitable for downstream mass spec analysis
|Applications||IP, CoIP, ChIP, RIP|
|Specificity/Target||Halo-tag (modified variant of the bacterial haloalkane dehalogenase enzyme from Rhodococcus rhodochrous) in the absence or presence of covalently bound chloralkane-based ligands.|
|Binding capacity||7.5-10 μg of recombinant Halo-tag per 25 μL bead slurry|
|Conjugate||Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)|
|Elution buffer||SDS sample buffer|
0.2 M glycine pH 2.5
|Wash buffer compatibility||4 M urea, 1 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100|
|Affinity (KD)||Dissociation constant KD of 2 nM|
|Compatibility with mass spectrometry||The Halo-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry|
|Storage Buffer||20% ethanol|
|Storage Condition||Upon receipt store at +4°C. Do not freeze!|
|SDS Halo-Trap Agarose (PDF)|
|Protocol Halo-Trap Agarose (PDF)|
|Troubleshooting guide immunoprecipitation (IP) (PDF)|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Anastasija (Verified Customer) (02-14-2022)
I used the Halo-trap agarose to perform nuclear co-IP of Halo-tagged proteins, and after a bit of optimization of the IP protocol I achieved very good pulldown efficiency with high amounts of bait and interactors, and low background. An added benefit is that upon elution there is no IgG co-eluting with the target proteins, which is normally a problem in conventional IP approaches using IgG. I am really happy with this product, and will continue using it in future experiments.