Cat no : mta
ChromoTek’s MK2-Trap Agarose are affinity beads for IP of MK2-fusion proteins. It comprises a MK2 Nanobody/ VHH conjugated to agarose beads.
|Description||Immunoprecipitation of MK2 with MK2-Trap Agarose.
• Fast, reliable & efficient one-step immunoprecipitation
• Short incubation time (1 h at 4°C)
• No heavy & light antibody chains
• Stable under harsh washing conditions
• Suitable for downstream mass spec analysis
|Specificity/Target||MK2 (MAP kinase-activated protein kinase 2, MAPKAP-K2)|
|Tested Reactivity||Human, Mouse, Hamster|
Posttranslational modifications: MK2-Trap recognizes unphosphorylated MK2 and Phospho-MK2 (Thr222). Specificity on Phospho-MK2 (Thr334) was not tested.
|Conjugate||Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)|
|Elution buffer||SDS sample buffer|
0.2 M glycine pH 2.5
|Compatibility with mass spectrometry||The MK2-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry|
|Storage Buffer||20% ethanol|
|Storage Condition||Upon receipt store at +4°C. Do not freeze!|
Proc Natl Acad Sci U S A
MK2 degradation as a sensor of signal intensity that controls stress-induced cell fate.
Alternative Translation Initiation Generates a Functionally Distinct Isoform of the Stress-Activated Protein Kinase MK2.