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ChromoTek TurboGFP-Trap Magnetic Agarose

TurboGFP-Trap Magnetic Agarose is an affinity reagent for immunoprecipitation (IP) of TurboGFP fusion proteins.

Specificity

TurboGFP, CopGFP, maxGFP

Applications

IP, CoIP

Conjugate

Magnetic agarose

Type

Nanobody

Cat no : tbtma

Synonyms

TurboGFP-Trap, TurboGFP, CopGFP, maxGFP,maxFP-Green, green fluorescent protein CopGFP, TurboGFP tag

Validation Data Gallery

  • Coomassie blue stained gel and Western blot.

    Coomassie blue stained gel and Western blot.

  • TurboGFP-Trap Magnetic Agarose for pull-down of TurboGFP fusion proteins: Coomassie and Western Blot Input (I), non-bound (FT), and bound (B).

    TurboGFP-Trap Magnetic Agarose for pull-down of TurboGFP fusion proteins: Coomassie and Western Blot Input (I), non-bound (FT), and bound (B).

Product Information

TurboGFP-Trap Magnetic Agarose is an affinity reagent for immunoprecipitation (IP) of TurboGFP fusion proteins.

DescriptionImmunoprecipitation of TurboGFP-fusion proteins and their interacting factors with anti-TurboGFP Nanobody conjugated to magnetic agarose beads.

• Fast, reliable & efficient one-step immunoprecipitation

• Ready-to-use

• No heavy & light antibody chains

• Stable under harsh washing conditions

• Suitable for downstream mass spec analysis

• Works in samples from: mammals, plants, bacteria, yeast, insects etc.

ApplicationsIP, CoIP
Specificity/TargetTurboGFP, CopGFP, maxGFP, maxFP-Green
For the complete list, please click here: Fluorescent protein specificity table
Binding capacity7.5-10 μg of recombinant TurboGFP per 25 μL bead slurry
ConjugateMagnetic agarose beads; bead size: ~40 µm (cross-linked 6 % magnetic agarose beads)
Elution bufferSDS sample buffer
0.1 mM citrate acid pH 3.0
Wash buffer compatibility6 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100, 0.2 % SDS
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) Dissociation constant KD of 0.5 nM
Compatibility with mass spectrometryThe TurboGFP-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2827597
Storage Buffer20% ethanol
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!

Publications

ApplicationTitle
CoIP

Mol Biol Cell

RUSC2 and WDR47 oppositely regulate kinesin-1-dependent distribution of ATG9A to the cell periphery.

Authors - Carlos M Guardia
View Article
IP

Front Immunol

SIRT2-PFKP interaction dysregulates phagocytosis in macrophages with acute ethanol-exposure

Authors - Anugraha Gandhirajan
View Article
CoIP

Development

CHD4 and SMYD1 repress common transcriptional programs in the developing heart

Authors - Wei Shi
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Xi (Verified Customer) (05-01-2024)

Worked well for HEK293 cells transfected with turboGFP-tagged constructs

  • Applications: Immunoprecipitation
  • Cell Tissue Type: HEK293
FH

Veronic (Verified Customer) (04-26-2024)

IPed Turbo-GFP fusion protein overexpressed in HEK293 cells. Great signal both in WB and MS Interactome analysis.

  • Applications: Immunoprecipitation
  • Cell Tissue Type: HEK203
FH

Peter (Verified Customer) (10-24-2022)

Excellent IP system for a GFP variant with limited reagents available

  • Applications: Immunoprecipitation
  • Cell Tissue Type: U2OS