Cat no : rta
RFP-Trap® Agarose is an affinity resin for IP of RFP-fusion proteins. It consists of an RFP Nanobody/ VHH coupled to agarose beads.
|Description||Immunoprecipitation of RFP-fusion proteins and their interacting factors with anti-RFP Nanobody conjugated to beads.
• Reliable and robust pull-down of mCherry and other RFP-fusion proteins
• No heavy & light antibody chains
• Stable under harsh washing conditions
• Suitable for downstream mass spec analysis
• Works in samples from: mammals, plants, bacteria, yeast, insects etc.
|Applications||IP, CoIP, ChIP, RIP|
|Specificity/Target||mRFP, mCherry, mRFPruby, mPlum, tagRFP, mKate2, mOrange, PA-mCherry, mScarlet |
For the complete list, please click here: Fluorescent protein specificity table
|Binding capacity||22.5 μg of recombinant RFP per 25 μL bead slurry|
|Conjugate||Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)|
|Elution buffer||SDS sample buffer|
0.2 M glycine pH 2.5
|Wash buffer compatibility||10 mM DTT, 4 M Urea, 2 M NaCl, 2 % Nonidet P40 Substitute, 1 % Triton X-100|
|Affinity (KD)||Dissociation constant KD of 5 nM|
|Compatibility with mass spectrometry||The RFP-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry|
|Storage Buffer||20% ethanol|
|Storage Condition||Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!|
|SDS RFP-Trap Agarose (PDF)|
|Protocol RFP-Trap Agarose (PDF)|
|Fluorescent protein specificity table (PDF)|
|Product brochure (PDF)|
|Chromatin Immunoprecipitation (ChIP) with RFP-tagged proteins|
|Split Fluorescent Protein Technology (PDF)|
|Troubleshooting guide immunoprecipitation (IP) (PDF)|
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A robust pipeline for rapid production of versatile nanobody repertoires.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Julie (Verified Customer) (07-07-2021)
I was using this kit to try a co-IP. My IP protein is tagged to cherry, but the very first step of IP did not work so I could not test for an interaction with other protein candidates. The antibody I used works very well. As you can see, the protein of interest is in the input and unbound fractions - meaning the RFP-protein did not bind to the RFP-trap agarose beads. Disappointed. I used buffers that were in the range that was recommended. It would be helpful if exact starting amounts of ingredients for buffers were provided since I can't test multiple conditions with a sample that only 2 reactions. I'm sure there is a way to troubleshoot, but again, I can't test multiple conditions with just 2 reactions and brain tissue is precious.