ChromoTek RFP-Trap Agarose

RFP-Trap® Agarose is an affinity resin for IP of RFP-fusion proteins. It consists of an RFP Nanobody/ VHH coupled to agarose beads.

Specificity

mCherry, mPlum, mRFP

Applications

IP, CoIP, ChIP, RIP

Conjugate

Agarose

Type

Nanobody

Cat no : rta

Synonyms

RFP, RFP-Trap, Red fluorescent protein, mCherry, mRFP, mRFPRuby, mKate2 , mPlum, mOrange, mRuby2, mScarlet-I, PA-mCherry, TagRFP, RFP tag



Product Information

RFP-Trap® Agarose is an affinity resin for IP of RFP-fusion proteins. It consists of an RFP Nanobody/ VHH coupled to agarose beads.

DescriptionImmunoprecipitation of RFP-fusion proteins and their interacting factors with anti-RFP Nanobody conjugated to beads.

• Reliable and robust pull-down of mCherry and other RFP-fusion proteins

• Ready-to-use

• No heavy & light antibody chains

• Stable under harsh washing conditions

• Suitable for downstream mass spec analysis

• Works in samples from: mammals, plants, bacteria, yeast, insects etc.

ApplicationsIP, CoIP, ChIP, RIP
Specificity/TargetmRFP, mCherry, mRFPruby, mPlum, tagRFP, mKate2, mOrange, PA-mCherry, mScarlet
For the complete list, please click here: Fluorescent protein specificity table
Binding capacity22.5 μg of recombinant RFP per 25 μL bead slurry
ConjugateAgarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5
Wash buffer compatibility10 mM DTT, 4 M Urea, 2 M NaCl, 2 % Nonidet P40 Substitute, 1 % Triton X-100
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) Dissociation constant KD of 5 nM
Compatibility with mass spectrometryThe RFP-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2631362
Storage Buffer20% ethanol
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!

Publications

ApplicationTitle

Nature

Genetic instability from a single S phase after whole-genome duplication.

Authors - Simon Gemble
IP

Science

Reconstitution of autophagosome nucleation defines Atg9 vesicles as seeds for membrane formation.

Authors - Justyna Sawa-Makarska
IP

Cell

Exome capture reveals ZNF423 and CEP164 mutations, linking renal ciliopathies to DNA damage response signaling.

Authors - Moumita Chaki
IP

Cell

Formation of NPR1 Condensates Promotes Cell Survival during the Plant Immune Response.

Authors - Raul Zavaliev
CoIP

Cell

A Defense Pathway Linking Plasma Membrane and Chloroplasts and Co-opted by Pathogens.

Authors - Laura Medina-Puche
IP

Nat Methods

A robust pipeline for rapid production of versatile nanobody repertoires.

Authors - Peter C Fridy

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Julie (Verified Customer) (07-07-2021)

I was using this kit to try a co-IP. My IP protein is tagged to cherry, but the very first step of IP did not work so I could not test for an interaction with other protein candidates. The antibody I used works very well. As you can see, the protein of interest is in the input and unbound fractions - meaning the RFP-protein did not bind to the RFP-trap agarose beads. Disappointed. I used buffers that were in the range that was recommended. It would be helpful if exact starting amounts of ingredients for buffers were provided since I can't test multiple conditions with a sample that only 2 reactions. I'm sure there is a way to troubleshoot, but again, I can't test multiple conditions with just 2 reactions and brain tissue is precious.

  • Applications: Other
  • Primary Antibody Dilution: IP: 1mg/ml, Western Blot: 1:1000
  • Cell Tissue Type: Cerebellar brain tissue expressing an cherry-tagged protein