Cat no : rta
RFP-Trap® Agarose is an affinity resin for IP of RFP-fusion proteins. It consists of an RFP Nanobody/ VHH coupled to agarose beads.
|Description||Immunoprecipitation of RFP-fusion proteins and their interacting factors with anti-RFP Nanobody conjugated to beads.
• Reliable and robust pull-down of mCherry and other RFP-fusion proteins
• No heavy & light antibody chains
• Stable under harsh washing conditions
• Suitable for downstream mass spec analysis
• Works in samples from: mammals, plants, bacteria, yeast, insects etc.
|Applications||IP, CoIP, ChIP, RIP|
|Specificity/Target||mRFP, mCherry, mRFPruby, mPlum, tagRFP, mKate2, mOrange, PA-mCherry, mScarlet |
For the complete list, please click here: Fluorescent protein specificity table
|Binding capacity||22.5 μg of recombinant RFP per 25 μL bead slurry|
|Conjugate||Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)|
|Elution buffer||SDS sample buffer|
0.2 M glycine pH 2.5
|Wash buffer compatibility||10 mM DTT, 4 M Urea, 2 M NaCl, 2 % Nonidet P40 Substitute, 1 % Triton X-100|
|Affinity (KD)||Dissociation constant KD of 5 nM|
|Compatibility with mass spectrometry||The RFP-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry|
|Storage Buffer||20% ethanol|
|Storage Condition||Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!|
|SDS RFP-Trap Agarose (PDF)|
|Protocol RFP-Trap Agarose (PDF)|
|Fluorescent protein specificity table (PDF)|
|Product brochure (PDF)|
|Chromatin Immunoprecipitation (ChIP) with RFP-tagged proteins|
|Split Fluorescent Protein Technology (PDF)|
|Troubleshooting guide immunoprecipitation (IP) (PDF)|
Genetic instability from a single S phase after whole-genome duplication.
Reconstitution of autophagosome nucleation defines Atg9 vesicles as seeds for membrane formation.
Exome capture reveals ZNF423 and CEP164 mutations, linking renal ciliopathies to DNA damage response signaling.
Formation of NPR1 Condensates Promotes Cell Survival during the Plant Immune Response.
A Defense Pathway Linking Plasma Membrane and Chloroplasts and Co-opted by Pathogens.
A robust pipeline for rapid production of versatile nanobody repertoires.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Andrea (Verified Customer) (10-05-2023)
Works well for Co-IP with RFP tag.
Julie (Verified Customer) (07-07-2021)
I was using this kit to try a co-IP. My IP protein is tagged to cherry, but the very first step of IP did not work so I could not test for an interaction with other protein candidates. The antibody I used works very well. As you can see, the protein of interest is in the input and unbound fractions - meaning the RFP-protein did not bind to the RFP-trap agarose beads. Disappointed. I used buffers that were in the range that was recommended. It would be helpful if exact starting amounts of ingredients for buffers were provided since I can't test multiple conditions with a sample that only 2 reactions. I'm sure there is a way to troubleshoot, but again, I can't test multiple conditions with just 2 reactions and brain tissue is precious.