ChromoTek GFP-Trap® Agarose
GFP-Trap® Agarose is an affinity resin for IP of GFP-fusion proteins. It consists of a GFP Nanobody/ VHH coupled to agarose beads.
Specificity
GFP, eGFP, CFP, YFP, BFP
Applications
IP, CoIP, ChIP, RIP
Conjugate
Agarose
3118
Cat no : gta
Synonyms
Validation Data Gallery
Product Information
GFP-Trap® Agarose is an affinity resin for IP of GFP-fusion proteins. It consists of a GFP Nanobody/ VHH coupled to agarose beads.
Applications | IP, CoIP, ChIP, RIP |
Clonality | Monoclonal |
Host | Alpaca |
Affinity | Dissociation constant KD of 1 pM |
Specificity/Target | AcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP A206K, CFP, YFP, Citrine, eCitrine, eYFP, Venus, Ypet, BFP |
Coupled VHH | Recombinant, monoclonal anti-Green Fluorescent Protein (GFP) single domain antibody (sdAb) fragment |
Conjugate | Agarose |
Beads | ~ 90 µm (cross-linked 4 % agarose beads) |
Binding capacity | 10 µL slurry bind about 12 µg of recombinant GFP |
Elution buffer | SDS sample buffer 0.2 M glycine pH 2.5 |
Storage Buffer | 20% ethanol |
Storage Condition | Upon receipt store at +4°C. Do not freeze! |
Documentation
SDS |
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SDS GFP-Trap Agarose |
Protocol |
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Protocol GFP-Trap Agarose (PDF) |
GFP-Trap 琼脂糖珠操作说明(中文) |
White papers |
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How to plan an Immunoprecipitation using the GFP-Trap |
Split Fluorescent Protein Technology (PDF) |
GFP-Trap as affinity tag |
Unique Stability of the ChromoTek GFP-Trap:GFP Complex |
GFP-Trap Specificity |
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Fluorescent protein specificity table |
Trouble shooting |
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Troubleshooting guide immunoprecipitation (IP) |
Brochure |
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GFP-Trap product brochure |
Publications
Application | Title |
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PLoS Genet Spinocerebellar ataxia type 11-associated alleles of Ttbk2 dominantly interfere with ciliogenesis and cilium stability. | |
Mol Biol Cell PRMT7 Methylates Eukaryotic Translation Initiation Factor 2α and Regulates its Role in Stress Granule Formation. | |
Life Sci Alliance BZLF1 interacts with chromatin remodelers promoting escape from latent infections with EBV. | |
Nat Commun The class 3 PI3K coordinates autophagy and mitochondrial lipid catabolism by controlling nuclear receptor PPARα. | |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Cristina (Verified Customer) (03-11-2022) | Great product. It is a daily must have in our lab.
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