ChromoTek GFP-Trap® Agarose

GFP-Trap® Agarose is an affinity resin for IP of GFP-fusion proteins. It consists of a GFP Nanobody/ VHH coupled to agarose beads.

Specificity

GFP and GFP derivates

Applications

IP, CoIP, ChIP, RIP

Type

Nanobody

Conjugate

Agarose

Cat no : gta

Synonyms

GFP tag, GFP, eGFP, eYFP, CFP, YFP, BFP, GFP-Trap, Green fluorescent protein, AcGFP, GFP S65T, mClover (Clover A260K), Monomeric EGFP A206K, pHluorin (ecliptic), PA-GFP, Superfolder GFP (sfGFP), TagGFP, TagGFP2, Citrine, Ecitrine, Venus, EYFP, Ypet



Product Information

GFP-Trap® Agarose is an affinity resin for IP of GFP-fusion proteins. It consists of a GFP Nanobody/ VHH coupled to agarose beads.

DescriptionImmunoprecipitation of GFP-fusion proteins and their interacting factors with anti-GFP Nanobody conjugated to beads.

• Fast, reliable & efficient one-step immunoprecipitation

• Ready-to-use

• No heavy & light antibody chains

• Stable under harsh washing conditions

• Suitable for downstream mass spec analysis

• Works in samples from: mammals, plants, bacteria, yeast, insects etc.

ApplicationsIP, CoIP, ChIP, RIP
Specificity/TargetAcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP A206K, CFP, YFP, Citrine, eCitrine, eYFP, Venus, Ypet, BFP
For the complete list, please click here: Fluorescent protein specificity table
Binding capacity25-30 µg of recombinant GFP per 25 µL bead slurry
ConjugateAgarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5
Wash buffer compatibility1 mM DTT, 3 M Guanidinium•HCl, 8 M Urea, 2 M NaCl, 2 % Nonidet P40 Substitute, 1 % SDS, 1 % Triton X-100
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) Dissociation constant KD of 1 pM
Compatibility with mass spectrometryThe GFP-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2631357
Storage Buffer20% ethanol
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!

Publications

ApplicationTitle
IP

Nature

Mammalian SUMO E3-ligases PIAS1 and PIAS4 promote responses to DNA double-strand breaks.

Authors - Yaron Galanty
IP

Nature

Oncogenically active MYD88 mutations in human lymphoma.

Authors - Vu N Ngo
IP

Nature

The prokaryote messenger c-di-GMP triggers stalk cell differentiation in Dictyostelium.

Authors - Zhi-hui Chen
CoIP

Nature

αTAT1 catalyses microtubule acetylation at clathrin-coated pits.

Authors - Guillaume Montagnac
CoIP

Nature

Inhibitory signalling to the Arp2/3 complex steers cell migration.

Authors - Irene Dang
IP

Nature

SHANK3 overexpression causes manic-like behaviour with unique pharmacogenetic properties.

Authors - Kihoon Han

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Rich (Verified Customer) (09-12-2022)

works well. A little on the pricey side but worth it.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: liver cancer cell lines
FH

Aditi (Verified Customer) (08-17-2022)

The product works very well even with non-conventional model systems. We used it for co-immunoprecipitation and mass spectrometry analysis. We used custom buffers and the product worked efficiently. The whole protocol is also quicker than regular IP protocols. Highly recommend!

  • Applications: Immunoprecipitation
  • Cell Tissue Type: Paramecium tetraurelia cells
FH

Benedikt (Verified Customer) (08-17-2022)

We did a pull-down of a ~131 GFP-fusionprotein using the recommended buffers and incubation times (1h) and immediately pulled our protein-of-interest. Some non-bound protein was still visible, although this might be due to high amount of input lysate (500 µg).

  • Applications: Western Blot,Immunoprecipitation,Other
  • Cell Tissue Type: Glioblastoma cell lines
FH

Peter (Verified Customer) (07-04-2022)

Our lab has used the GFP-Trap Agarose beads on many occasions to successfully co-immunoprecipitate GFP fusion proteins for Mass Spectrometry and for RNA sequencing (RIP-Seq). We even managed to pulldown enough of one protein to detect it after the western blot on the PVDF membrane with amidoblack staining.

  • Applications: Western Blot,Immunoprecipitation
  • Cell Tissue Type: C. elegans lysate
FH

Cristina (Verified Customer) (06-10-2022)

we have been using this product for years and it's one of the fav in the lab

  • Applications: Immunoprecipitation
  • Cell Tissue Type: mamallian
FH

George (Verified Customer) (06-10-2022)

Used this for GFP immunoprecipitations from HeLa cells. Product worked as described and is much quicker and more effective than conventional immunoprecipitations. Would highly recommend.

  • Applications: Immunoprecipitation
  • Cell Tissue Type: HeLa
FH

Cristina (Verified Customer) (03-11-2022)

Great product. It is a daily must have in our lab.

  • Applications: Immunoprecipitation