ChromoTek GFP-Trap® Agarose

GFP-Trap® Agarose is an affinity resin for IP of GFP-fusion proteins. It consists of a GFP Nanobody/ VHH coupled to agarose beads.

Specificity

GFP and GFP derivates

Applications

IP, CoIP, ChIP, RIP

Type

Nanobody

Conjugate

Agarose

Publications

3364

Reviews (9)

Cat no : gta

Synonyms

GFP tag, GFP, eGFP, eYFP, CFP, YFP, BFP, GFP-Trap, Green fluorescent protein, AcGFP, GFP S65T, mClover (Clover A260K), Monomeric EGFP A206K, pHluorin (ecliptic), PA-GFP, Superfolder GFP (sfGFP), TagGFP, TagGFP2, Citrine, Ecitrine, Venus, EYFP, Ypet

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Validation Data Gallery

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  Immunoprecipitation of GFP with GFP-Trap Agarose beads. I: Input, FT: Flow-Through, B: Bound.

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Product Information

GFP-Trap® Agarose is an affinity resin for IP of GFP-fusion proteins. It consists of a GFP Nanobody/ VHH coupled to agarose beads.

• Description: Immunoprecipitation of GFP-fusion proteins and their interacting factors with anti-GFP Nanobody conjugated to beads.

  • Fast, reliable & efficient one-step immunoprecipitation

  • Ready-to-use

  • No heavy & light antibody chains

  • Stable under harsh washing conditions

  • Suitable for downstream mass spec analysis

  • Works in samples from: mammals, plants, bacteria, yeast, insects etc.

• Applications: IP, CoIP, ChIP, RIP
• Specificity/Target: AcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP A206K, CFP, YFP, Citrine, eCitrine, eYFP, Venus, Ypet, BFP ; For the complete list, please click here: Fluorescent protein specificity table
• Binding capacity: 25-30 µg of recombinant GFP per 25 µL bead slurry
• Conjugate: Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
• Elution buffer: SDS sample buffer; 0.2 M glycine pH 2.5
• Wash buffer compatibility: 1 mM DTT, 3 M Guanidinium•HCl, 8 M Urea, 2 M NaCl, 2 % Nonidet P40 Substitute, 1 % SDS, 1 % Triton X-100
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity (KD): Dissociation constant K_D of 1 pM
• Compatibility with mass spectrometry: The GFP-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
• RRID: AB_2631357
• Storage Buffer: 20% ethanol
• Storage Condition: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!

Other Formats

GFP-Trap Agarose Kit

GFP-Trap Magnetic Agarose

GFP-Trap Magnetic Agarose kit

GFP-Trap Magnetic Particles M-270

GFP-Trap Magnetic Particles M-270 kit

GFP-Trap Multiwell Plate

Publications

Application	Title
	Nature; Principles of mitoribosomal small subunit assembly in eukaryotes; Authors - Nathan J Harper
IP	Nature; Cell surface and intracellular auxin signalling for H+ fluxes in root growth.; Authors - Lanxin Li
IP	Nature; TMK-based cell-surface auxin signalling activates cell-wall acidification.; Authors - Wenwei Lin
IP	Nature; Activation of homologous recombination in G1 preserves centromeric integrity.; Authors - Duygu Yilmaz
CoIP	Nature; Phytocytokine signalling reopens stomata in plant immunity and water loss.; Authors - Zunyong Liu
IP	Nature; Ligand-induced monoubiquitination of BIK1 regulates plant immunity.; Authors - Xiyu Ma

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Alessandro (Verified Customer) (01-19-2023)

great binding

• Applications: Immunoprecipitation

FH

Liam (Verified Customer) (01-11-2023)

Easy to use and produces clean/reliable results. Over the past three years, the ChromoTek GFP-Trap® Agarose have become a core experimental resource for our lab.

• Applications: Western Blot
• Cell Tissue Type: HEK 293T

FH

Rich (Verified Customer) (09-12-2022)

works well. A little on the pricey side but worth it.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: liver cancer cell lines

FH

Aditi (Verified Customer) (08-17-2022)

The product works very well even with non-conventional model systems. We used it for co-immunoprecipitation and mass spectrometry analysis. We used custom buffers and the product worked efficiently. The whole protocol is also quicker than regular IP protocols. Highly recommend!

• Applications: Immunoprecipitation
• Cell Tissue Type: Paramecium tetraurelia cells

FH

Benedikt (Verified Customer) (08-17-2022)

We did a pull-down of a ~131 GFP-fusionprotein using the recommended buffers and incubation times (1h) and immediately pulled our protein-of-interest. Some non-bound protein was still visible, although this might be due to high amount of input lysate (500 µg).

• Applications: Western Blot,Immunoprecipitation,Other
• Cell Tissue Type: Glioblastoma cell lines

FH

Peter (Verified Customer) (07-04-2022)

Our lab has used the GFP-Trap Agarose beads on many occasions to successfully co-immunoprecipitate GFP fusion proteins for Mass Spectrometry and for RNA sequencing (RIP-Seq). We even managed to pulldown enough of one protein to detect it after the western blot on the PVDF membrane with amidoblack staining.

• Applications: Western Blot,Immunoprecipitation
• Cell Tissue Type: C. elegans lysate

FH

Cristina (Verified Customer) (06-10-2022)

we have been using this product for years and it's one of the fav in the lab

• Applications: Immunoprecipitation
• Cell Tissue Type: mamallian

FH

George (Verified Customer) (06-10-2022)

Used this for GFP immunoprecipitations from HeLa cells. Product worked as described and is much quicker and more effective than conventional immunoprecipitations. Would highly recommend.

• Applications: Immunoprecipitation
• Cell Tissue Type: HeLa

FH

Cristina (Verified Customer) (03-11-2022)

Great product. It is a daily must have in our lab.

• Applications: Immunoprecipitation