ChromoTek GFP-Trap® Agarose

GFP-Trap® Agarose is an affinity resin for IP of GFP-fusion proteins. It consists of a GFP Nanobody/ VHH coupled to agarose beads.

Specificity

GFP, eGFP, CFP, YFP, BFP

Applications

IP, CoIP, ChIP, RIP

Conjugate

Agarose

Publications

3118

Reviews (1)

Cat no : gta

Synonyms

GFP tag, GFP, eGFP, eYFP, CFP, YFP, BFP, GFP-Trap, Green fluorescent protein, AcGFP, GFP S65T, mClover (Clover A260K), Monomeric EGFP A206K, pHluorin (ecliptic), PA-GFP, Superfolder GFP (sfGFP), TagGFP, TagGFP2, Citrine, Ecitrine, Venus, EYFP, Ypet

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Validation Data Gallery

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  Immunoprecipitation of GFP with GFP-Trap Agarose beads. I: Input, FT: Flow-Through, B: Bound.

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Product Information

GFP-Trap® Agarose is an affinity resin for IP of GFP-fusion proteins. It consists of a GFP Nanobody/ VHH coupled to agarose beads.

• Applications: IP, CoIP, ChIP, RIP
• Clonality: Monoclonal
• Host: Alpaca
• Affinity: Dissociation constant KD of 1 pM
• Specificity/Target: AcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP A206K, CFP, YFP, Citrine, eCitrine, eYFP, Venus, Ypet, BFP
• Coupled VHH: Recombinant, monoclonal anti-Green Fluorescent Protein (GFP) single domain antibody (sdAb) fragment
• Conjugate: Agarose
• Beads: ~ 90 µm (cross-linked 4 % agarose beads)
• Binding capacity: 10 µL slurry bind about 12 µg of recombinant GFP
• Elution buffer: SDS sample buffer; 0.2 M glycine pH 2.5
• Storage Buffer: 20% ethanol
• Storage Condition: Upon receipt store at +4°C. Do not freeze!

Publications

Application	Title
IP	PLoS Genet; Spinocerebellar ataxia type 11-associated alleles of Ttbk2 dominantly interfere with ciliogenesis and cilium stability.; Authors - Emily Bowie
IP	Mol Biol Cell; PRMT7 Methylates Eukaryotic Translation Initiation Factor 2α and Regulates its Role in Stress Granule Formation.; Authors - Nasim Haghandish
CoIP	Nat Commun; Folliculin regulates mTORC1/2 and WNT pathways in early human pluripotency.; Authors - J Mathieu
IP	Life Sci Alliance; BZLF1 interacts with chromatin remodelers promoting escape from latent infections with EBV.; Authors - Marisa Schaeffner
IP	Nat Commun; The class 3 PI3K coordinates autophagy and mitochondrial lipid catabolism by controlling nuclear receptor PPARα.; Authors - Anton Iershov
CoIP	Cell Rep; The ER-Localized Protein DFCP1 Modulates ER-Lipid Droplet Contact Formation.; Authors - Dongfang Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Cristina (Verified Customer) (03-11-2022)

Great product. It is a daily must have in our lab.

• Applications: Immunoprecipitation