ChromoTek GFP-Trap® Agarose
GFP-Trap® Agarose is an affinity resin for IP of GFP-fusion proteins. It consists of a GFP Nanobody/ VHH coupled to agarose beads.
Specificity
GFP and GFP derivates
Applications
IP, CoIP, ChIP, RIP
Type
Nanobody
Conjugate
Agarose
3218
Cat no : gta
Synonyms
Validation Data Gallery
Product Information
GFP-Trap® Agarose is an affinity resin for IP of GFP-fusion proteins. It consists of a GFP Nanobody/ VHH coupled to agarose beads.
| Description | Immunoprecipitation of GFP-fusion proteins and their interacting factors with anti-GFP Nanobody conjugated to beads.
• Fast, reliable & efficient one-step immunoprecipitation • Ready-to-use • No heavy & light antibody chains • Stable under harsh washing conditions • Suitable for downstream mass spec analysis • Works in samples from: mammals, plants, bacteria, yeast, insects etc. |
| Applications | IP, CoIP, ChIP, RIP |
| Specificity/Target | AcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP A206K, CFP, YFP, Citrine, eCitrine, eYFP, Venus, Ypet, BFP For the complete list, please click here: Fluorescent protein specificity table |
| Binding capacity | 25-30 µg of recombinant GFP per 25 µL bead slurry |
| Conjugate | Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads) |
| Elution buffer | SDS sample buffer 0.2 M glycine pH 2.5 |
| Wash buffer compatibility | 1 mM DTT, 3 M Guanidinium•HCl, 8 M Urea, 2 M NaCl, 2 % Nonidet P40 Substitute, 1 % SDS, 1 % Triton X-100 |
| Type | Nanobody |
| Class | Recombinant |
| Host | Alpaca |
| Affinity (KD) | Dissociation constant KD of 1 pM |
| Compatibility with mass spectrometry | The GFP-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry |
| RRID | AB_2631357 |
| Storage Buffer | 20% ethanol |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze! |
Documentation
| SDS |
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| SDS GFP-Trap Agarose |
| Protocol |
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| Protocol GFP-Trap Agarose (PDF) |
| GFP-Trap 琼脂糖珠操作说明（中文） |
| White papers |
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| How to plan an Immunoprecipitation using the GFP-Trap |
| Split Fluorescent Protein Technology (PDF) |
| GFP-Trap as affinity tag |
| Unique Stability of the ChromoTek GFP-Trap:GFP Complex |
| GFP-Trap Specificity |
|---|
| Fluorescent protein specificity table |
| Trouble shooting |
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| Troubleshooting guide immunoprecipitation (IP) |
| Brochure |
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| GFP-Trap product brochure |
Publications
| Application | Title |
|---|---|
Nature Mammalian SUMO E3-ligases PIAS1 and PIAS4 promote responses to DNA double-strand breaks. | |
Nature The prokaryote messenger c-di-GMP triggers stalk cell differentiation in Dictyostelium. | |
Nature SHANK3 overexpression causes manic-like behaviour with unique pharmacogenetic properties. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Rich (Verified Customer) (09-12-2022) | works well. A little on the pricey side but worth it.
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FH Aditi (Verified Customer) (08-17-2022) | The product works very well even with non-conventional model systems. We used it for co-immunoprecipitation and mass spectrometry analysis. We used custom buffers and the product worked efficiently. The whole protocol is also quicker than regular IP protocols. Highly recommend!
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FH Benedikt (Verified Customer) (08-17-2022) | We did a pull-down of a ~131 GFP-fusionprotein using the recommended buffers and incubation times (1h) and immediately pulled our protein-of-interest. Some non-bound protein was still visible, although this might be due to high amount of input lysate (500 µg).
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FH Peter (Verified Customer) (07-04-2022) | Our lab has used the GFP-Trap Agarose beads on many occasions to successfully co-immunoprecipitate GFP fusion proteins for Mass Spectrometry and for RNA sequencing (RIP-Seq). We even managed to pulldown enough of one protein to detect it after the western blot on the PVDF membrane with amidoblack staining.
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FH Cristina (Verified Customer) (06-10-2022) | we have been using this product for years and it's one of the fav in the lab
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FH George (Verified Customer) (06-10-2022) | Used this for GFP immunoprecipitations from HeLa cells. Product worked as described and is much quicker and more effective than conventional immunoprecipitations. Would highly recommend.
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FH Cristina (Verified Customer) (03-11-2022) | Great product. It is a daily must have in our lab.
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