ChromoTek Myc-Trap® Magnetic Agarose

The ChromoTek Myc-Trap® Magnetic Agarose is an affinity resin for IP of Myc-fusion proteins. It consists of a Myc Nanobody/VHH coupled to magnetic agarose beads.

Specificity

Myc-Tag

Applications

IP, CoIP, ChIP, RIP

Conjugate

Magnetic agarose

Type

Nanobody

Cat no : ytma

Synonyms

Myc tag, Myc, Myc-trap, EQKLISEEDL



Product Information

The ChromoTek Myc-Trap® Magnetic Agarose is an affinity resin for IP of Myc-fusion proteins. It consists of a Myc Nanobody/VHH coupled to magnetic agarose beads.

DescriptionImmunoprecipitation of Myc-tagged proteins and their interacting factors with anti-Myc Nanobody conjugated to beads.

• Fast, reliable & efficient one-step immunoprecipitation

• Ready-to-use

• No heavy & light antibody chains

• Stable under harsh washing conditions

• Suitable for downstream mass spec analysis

ApplicationsIP, CoIP, ChIP, RIP
Specificity/TargetMyc-tag sequence motif EQKLISEEDL at the N-terminus, C-terminus, or internal site of the fusion protein. Endogenous c-myc is NOT bound.
Binding capacity17.5 μg of recombinant Myc-tagged protein (~42 kDa) per 25 μL bead slurry
ConjugateMagnetic agarose beads; bead size: ~40 µm (cross-linked 6 % magnetic agarose beads)
Elution buffer2x Myc peptide.
SDS sample buffer
0.2 M glycine pH 2.5
Wash buffer compatibility2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) 1x Myc-tag: Dissociation constant KD of 500 nM
2x Myc-tag: Dissociation constant KD of 0.5 nM
Compatibility with mass spectrometryThe Myc-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2631370
Storage Buffer20% ethanol
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!

Publications

ApplicationTitle
IP

Cell

Human-Specific NOTCH2NL Genes Expand Cortical Neurogenesis through Delta/Notch Regulation.

Authors - Ikuo K Suzuki
IP

Cell

A Bacterial Effector Mimics a Host HSP90 Client to Undermine Immunity.

Authors - Victor A Lopez
IP

Cell Metab

Insulin and Leptin/Upd2 Exert Opposing Influences on Synapse Number in Fat-Sensing Neurons.

Authors - Ava E Brent
IP

Cell Host Microbe

CD31 (PECAM-1) Serves as the Endothelial Cell-Specific Receptor of Clostridium perfringens β-Toxin.

Authors - Julia Bruggisser
IP

Nat Microbiol

LncRNA DINOR is a virulence factor and global regulator of stress responses in Candida auris.

Authors - Jiaxin Gao
IP

Sci Adv

Two interacting transcriptional coactivators cooperatively control plant immune responses.

Authors - Huan Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Amy (Verified Customer) (06-10-2022)

Clean and efficient precipitation of Myc-tagged bait proteins, HEK293T cells transfected with Myc-tagged proteins and lysed in RIPA buffer prior to precipitation with Myc-Trap magnetic agarose beads.

  • Applications: Immunoprecipitation
  • Cell Tissue Type: HEK293T