ChromoTek GFP-Trap® Magnetic Agarose

The ChromoTek GFP-Trap® Magnetic Agarose are affinity beads for IP of GFP-fusion proteins. It comprises a GFP Nanobody/ VHH coupled to magnetic agarose beads.

Specificity

GFP and GFP derivates

Applications

IP, CoIP, ChIP, RIP

Conjugate

Magnetic agarose

Type

Nanobody

Cat no : gtma

Synonyms

GFP tag, GFP, eGFP, eYFP, CFP, YFP, BFP, GFP-Trap, Green fluorescent protein, AcGFP, GFP S65T, mClover (Clover A260K), Monomeric EGFP A206K, pHluorin (ecliptic), PA-GFP, Superfolder GFP (sfGFP), TagGFP, TagGFP2, Citrine, Ecitrine, Venus, EYFP, Ypet



Product Information

The ChromoTek GFP-Trap® Magnetic Agarose are affinity beads for IP of GFP-fusion proteins. It comprises a GFP Nanobody/ VHH coupled to magnetic agarose beads.

DescriptionImmunoprecipitation of GFP-fusion proteins and their interacting factors with anti-GFP Nanobody conjugated to magnetic agarose beads.

• Fast, reliable & efficient one-step immunoprecipitation

• Ready-to-use

• No heavy & light antibody chains

• Stable under harsh washing conditions

• Suitable for downstream mass spec analysis

• Works in samples from: mammals, plants, bacteria, yeast, insects etc.

ApplicationsIP, CoIP, ChIP, RIP
Specificity/TargetAcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP A206K, CFP, YFP, Citrine, eCitrine, eYFP, Venus, Ypet, BFP
For the complete list, please click here: Fluorescent protein specificity table
Binding capacity15-20 μg of recombinant GFP per 25 μL bead slurry
ConjugateMagnetic agarose beads; bead size: ~40 µm (cross-linked 6 % magnetic agarose beads)
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5
Wash buffer compatibility1 mM DTT, 3 M Guanidinium•HCl, 8 M Urea, 2 M NaCl, 2 % Nonidet P40 Substitute, 1 % SDS, 1 % Triton X-100
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) Dissociation constant KD of 1 pM
Compatibility with mass spectrometryThe GFP-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2631358
Storage Buffer20% ethanol
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!

Publications

ApplicationTitle
IP

Nature

A pathway coordinated by DELE1 relays mitochondrial stress to the cytosol.

Authors - Evelyn Fessler
IP

Nature

Mitochondrial stress is relayed to the cytosol by an OMA1-DELE1-HRI pathway.

Authors - Xiaoyan Guo
IP

Nature

Distinct proteostasis circuits cooperate in nuclear and cytoplasmic protein quality control.

Authors - Rahul S Samant
IP

Nature

Cold-induced Arabidopsis FRIGIDA nuclear condensates for FLC repression.

Authors - Pan Zhu
IP

Nature

POLAR-guided signalling complex assembly and localization drive asymmetric cell division.

Authors - Anaxi Houbaert
IP

Nature

Gamete fusion triggers bipartite transcription factor assembly to block re-fertilization.

Authors - Aleksandar Vještica

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Amy (Verified Customer) (09-02-2022)

Efficient pull down of GFP-tagged protein from HEK293T cell lysates.

  • Applications: Immunoprecipitation
FH

Rich (Verified Customer) (07-26-2022)

  • Applications: Western Blot,Immunoprecipitation
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: liver
FH

James (Verified Customer) (06-16-2022)

Overexpressed GFP in HEK293T cells - efficient pulldown with GFP beads.

  • Applications: Immunoprecipitation
  • Cell Tissue Type: HEK293T