ChromoTek GFP-Trap® Magnetic Agarose

The ChromoTek GFP-Trap® Magnetic Agarose are affinity beads for IP of GFP-fusion proteins. It comprises a GFP Nanobody/ VHH coupled to magnetic agarose beads.

Specificity

GFP and GFP derivates

Applications

IP, CoIP, ChIP, RIP

Conjugate

Magnetic agarose

Type

Nanobody

Publications

1353

Reviews (10)

Cat no : gtma

Synonyms

GFP tag, GFP, eGFP, eYFP, CFP, YFP, BFP, GFP-Trap, Green fluorescent protein, AcGFP, GFP S65T, mClover (Clover A260K), Monomeric EGFP A206K, pHluorin (ecliptic), PA-GFP, Superfolder GFP (sfGFP), TagGFP, TagGFP2, Citrine, Ecitrine, Venus, EYFP, Ypet

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Validation Data Gallery

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  GFP-Trap Magnetic Agarose for immunoprecipitation of GFP-fusion proteins. Note single-band in bound fraction, which indicates a very effective pull-down. I: Input, FT: Flow-Through, B: Bound.

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Product Information

The ChromoTek GFP-Trap® Magnetic Agarose are affinity beads for IP of GFP-fusion proteins. It comprises a GFP Nanobody/ VHH coupled to magnetic agarose beads.

• Description: Immunoprecipitation of GFP-fusion proteins and their interacting factors with anti-GFP Nanobody conjugated to magnetic agarose beads.

  • Fast, reliable & efficient one-step immunoprecipitation

  • Ready-to-use

  • No heavy & light antibody chains

  • Stable under harsh washing conditions

  • Suitable for downstream mass spec analysis

  • Works in samples from: mammals, plants, bacteria, yeast, insects etc.

• Applications: IP, CoIP, ChIP, RIP
• Specificity/Target: AcGFP, Clover, eGFP, Emerald, GFP, GFP5, GFP Envy, GFP S65T, mGFP, mPhluorin, PA-GFP, Superfolder GFP, TagGFP, TagGFP2, monomeric eGFP A206K, CFP, YFP, Citrine, eCitrine, eYFP, Venus, Ypet, BFP ; For the complete list, please click here: Fluorescent protein specificity table
• Binding capacity: 15-20 μg of recombinant GFP per 25 μL bead slurry
• Conjugate: Magnetic agarose beads; bead size: ~40 µm (cross-linked 6 % magnetic agarose beads)
• Elution buffer: SDS sample buffer; 0.2 M glycine pH 2.5
• Wash buffer compatibility: 1 mM DTT, 3 M Guanidinium•HCl, 8 M Urea, 2 M NaCl, 2 % Nonidet P40 Substitute, 1 % SDS, 1 % Triton X-100
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity (KD): Dissociation constant K_D of 1 pM
• Compatibility with mass spectrometry: The GFP-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
• RRID: AB_2631358
• Storage Buffer: 20% ethanol
• Storage Condition: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!

Other Formats

GFP-Trap Agarose

GFP-Trap Agarose kit

GFP-Trap Magnetic Agarose kit

GFP-Trap Magnetic Particles M-270

GFP-Trap Magnetic Particles M-270 kit

GFP-Trap Multiwell Plate

Publications

Application	Title
CoIP	Nature; Reducing brassinosteroid signalling enhances grain yield in semi-dwarf wheat; Authors - Long Song
IP	Nature; Polθ is phosphorylated by PLK1 to repair double-strand breaks in mitosis; Authors - Camille Gelot
IP	Cell; STING orchestrates the neuronal inflammatory stress response in multiple sclerosis; Authors - Marcel S Woo
IP	Cell; Pathogen protein modularity enables elaborate mimicry of a host phosphatase; Authors - Hui Li
ChIP	Cell; Chromatin remodeling of histone H3 variants by DDM1 underlies epigenetic inheritance of DNA methylation; Authors - Seung Cho Lee
IP	Nature; A pathway coordinated by DELE1 relays mitochondrial stress to the cytosol.; Authors - Evelyn Fessler

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Haifei (Verified Customer) (09-13-2024)

Easily followed protocol. Clean results.

• Applications: Immunoprecipitation
• Cell Tissue Type: 293T

FH

Alen (Verified Customer) (08-20-2024)

Great and convenient product

• Applications: Immunoprecipitation
• Cell Tissue Type: Protein

FH

Margaux (Verified Customer) (06-11-2024)

I used GFP-TRAP to isolate GFP-tagged proteins. It was very efficient and it's a real advantage not to have to deal with heavy and light antibody chains.

• Applications: Immunoprecipitation
• Cell Tissue Type: HeLa cells

FH

Titouan (Verified Customer) (06-11-2024)

Very efficient pull-down of GFP-tagged protein from cell lysates (293T, Hela...) and very easy to use

• Applications: Immunoprecipitation
• Cell Tissue Type: HEK293T

FH

Mona (Verified Customer) (08-03-2023)

I purified GF tagged protein and efficiency was almost 100%

• Applications: Protein purification
• Cell Tissue Type: HeLa cell line

FH

Rhys (Verified Customer) (11-08-2022)

Used for IP of a eGFP tagged construct, easy to use and excellent efficiency.

• Applications: Immunoprecipitation

FH

Peter (Verified Customer) (10-24-2022)

Best system I have used for GFP IPs

• Applications: Immunoprecipitation
• Cell Tissue Type: U2OS

FH

Amy (Verified Customer) (09-02-2022)

Efficient pull down of GFP-tagged protein from HEK293T cell lysates.

• Applications: Immunoprecipitation

FH

Rich (Verified Customer) (07-26-2022)

• Applications: Western Blot,Immunoprecipitation
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: liver

FH

James (Verified Customer) (06-16-2022)

Overexpressed GFP in HEK293T cells - efficient pulldown with GFP beads.

• Applications: Immunoprecipitation
• Cell Tissue Type: HEK293T