Cat no : yta
Myc-Trap® Agarose is an affinity reagent for IP and purification of Myc-tagged proteins. It consists of a Myc Nanobody/ VHH coupled to agarose beads.
|Description||Immunoprecipitation of Myc-tagged proteins and their interacting factors with anti-Myc Nanobody conjugated to beads.
• Fast, reliable & efficient one-step immunoprecipitation
• Elution with peptide
• No heavy & light antibody chains
• Stable under harsh washing conditions
• Suitable for downstream mass spec analysis
|Applications||IP, CoIP, ChIP, RIP|
|Specificity/Target||Myc-tag sequence motif EQKLISEEDL at the N-terminus, C-terminus, or internal site of the fusion protein. Endogenous c-myc is NOT bound.|
|Binding capacity||17.5 μg of recombinant Myc-tagged protein (~42 kDa) per 25 μL bead slurry|
|Conjugate||Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)|
|Elution buffer||2x Myc peptide.|
SDS sample buffer
0.2 M glycine pH 2.5
|Wash buffer compatibility||2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100|
|Affinity (KD)||1x Myc-tag: Dissociation constant KD of 500 nM|
2x Myc-tag: Dissociation constant KD of 0.5 nM
|Compatibility with mass spectrometry||The Myc-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry|
|Storage Buffer||20% ethanol|
|Storage Condition||Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!|
|SDS Myc-Trap Agarose (PDF)|
|Protocol Myc-Trap Agarose (PDF)|
|Myc-Trap and anti-Myc antibody flyer (PDF)|
|Myc-Trap versus conventional anti-Myc antibodies (PDF)|
|Tips & tricks for immunoprecipitation using the Myc-Trap (PDF)|
|Troubleshooting guide immunoprecipitation (PDF)|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Frédéric (Verified Customer) (05-05-2022)
I'm very satisfied with the Myc-Trap agarose. The result is excellent and it is easy to use.