1X10^6 untreated or LPS-treated mouse splenocytes were surface stained with 0.2 ug PE Anti-Mouse CD86 (PE-65068, Clone: GL1). Cells were not fixed.
1X10^6 untreated (dashed line) or LPS-treated mouse splenocytes were surface stained with 0.2 ug PE Anti-Mouse CD86 (PE-65068, Clone: GL1) (red), or 0.2 ug Isotype Control (blue). Cells were not fixed.
1x10^6 human PBMCs were surface stained with 5 ul PE HLA-DR Recombinant Antibody (PE-65560, Clone:L243) or PE Mouse IgG2a Isotype Control (C1.18.4) (PE-65208, Clone: C1.18.4), and 5 ul CoraLite® Plus 647 Anti-Human CD19 (HIB19) (CL647-65110, Clone: HIB19). Cells were not fixed.
1X10^6 Balb/c mouse peritoneal macrophages were surface stained with 0.5 ug PE Anti-Mouse CD80 (B7-1) (PE-65076, Clone:16-10A1) (red), or 0.5 ug Isotype Control. Cells were not fixed.
1X10^6 unstimulated or PMA and Ionomycin stimulated (in the presence of Brefeldin A) C57BL/6 mouse splenocytes were surface stained with CoraLite® Plus 647 Anti-Mouse CD3 (CL647-65077, Clone: 17A2) and then fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). Cells were then stained with 0.5 ug PE Anti-Mouse IFN gamma (PE-65153, Clone: XMG1.2).
1X10^6 mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD3 and 0.5 ug PE Anti-Mouse CD8a (PE-65069, Clone:53-6.7) or 0.5 ug Control Antibody. Cells were not fixed.
Human monocyte-derived dendritic cells (monocytes stimulated with GM-CSF and IL-4) were surface stained with 10 ul PE Anti-Human CD206 (PE-65155, Clone:15-2) (red) or PE-Mouse IgG1 isotype control (black). Cells were not fixed.
1X10^6 human whole blood cells were surface stained with PE Anti-Human STRO-1 (PE-65184, Clone:STRO-1). Cells were not fixed. CD45 dim, CD34 positive lymphocytes were gated.
1X10^6 mouse splenocytes were surface co-stained with CoraLite® Plus 647 Anti-Mouse CD3 and 0.2 ug PE Anti-Mouse CD4 (PE-65104, Clone:GK1.5) or 0.2 ug Isotype Control. Cells were not fixed.
1X10^6 human PBMCs were surface stained with 5 ul PE Anti-Human CD3 (PE-65133, Clone:OKT3), or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.
1X10^6 human PBMCs were surface stained with 5 ul PE Anti-Human CD3 (PE-65133, Clone: OKT3), or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.
100 ul human peripheral blood were surface stained with 20.00 ul PE Anti-Human CD34 (PE-65183, Clone: QBEnd-10) and 5 ul APC Anti-Human CD45 (APC-65109, Clone: HI30), and then treated with red blood cell lysis buffer. Cells were not fixed.
1X10^6 mouse splenocytes were surface co-stained with Anti-Mouse CD4 and 0.5 ug PE Anti-Mouse CD3 (PE-65077, Clone: 17A2) (red) or 0.5 ug Isotype Control. Cells were not fixed.
1X10^6 mouse splenocytes were surface stained with 0.5 ug PE Anti-Mouse CD3 (PE-65077, Clone:17A2) (red), or 0.5 ug Isotype Control. Cells were not fixed.
1X10^6 unstimulated and anti-CD3/CD28-stimulated (with 50 ng/ml TGF-β, 3 days) mouse splenocytes were surface stained with CoraLite® Plus 647-conjugated Anti-Mouse CD4. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011), and then intracellularly stained with 0.06 ug PE Anti-Mouse Foxp3 (PE-65089, Clone:3G3).
1X10^6 human PBMCs were surface stained with 5 ul PE Anti-Human CD45 (PE-65109, Clone:HI30) (red) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.
1X10^6 human PBMCs were surface co-stained with Atlantic Blue™ Anti-Human CD3 and 5 ul PE Anti-Human CD8 (PE-65146, Clone:SK1), or PE Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.
1X10^6 human PBMCs were surface stained with 5 ul PE Anti-Human CD8 (PE-65146, Clone:SK1), or PE Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.
1X10^6 mouse splenocytes cells were stained with 0.20ug CD274 (PD-L1, B7-H1) antibody (PE-65073, red) and control antibody (blue). Cells were not fixed.
1X10^6 C57BL/6 mouse splenocytes were surface co-stained with APC Anti-Mouse CD3 (17A2) and 0.25 ug PE Anti-Mouse CD4 (PE-65141, Clone:RM4-5) or 0.25 ug PE Rat IgG2a Isotype Control. Cells were not fixed.
1X10^6 human PBMCs were surface stained with 0.2 ug PE Anti-Mouse IgG2a Isotype Control (PE-65208, Clone:C1.18.4) (red), or PE Anti-Human CD3 (OKT3) (green), or unstained (blue). Cells were not fixed. Lymphocytes were gated.
1x10^6 human PBMCs were surface stained with 5 ul PE Anti-Human CD11b (PE-65116, Clone:ICRF44), or PE Mouse IgG1 Isotype Control. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.
1x10^6 human PBMCs were surface stained with 5 ul PE Anti-Human CD11b (PE-65116, Clone:ICRF44), or PE Mouse IgG1 Isotype Control. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. Monocytes were gated.
1X10^6 human PBMCs were treated with FC receptor block then surface stained with CL405 Anti-Human CD14 and 5 ul PE Anti-Human CD163 (PE-65169, Clone:GHI/61) or PE Mouse IgG1 Isotype Control. Cells were not fixed. Monocytes were gated.
1X10^6 human PBMCs were treated with FC receptor block then surface stained with 5 ul PE Anti-Human CD163 (PE-65169, Clone:GHI/61) or PE Mouse IgG1 Isotype Control. Cells were not fixed. Monocytes were gated.