PE Anti-Human CD146 (P1H12)

CD146 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Rabbit, Canine, Mouse, Human and More (1)

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

P1H12

Cat no : PE-65181

Synonyms

CD146, MCAM, MUC18



Tested Applications

Positive FC detected inA375 cells

Recommended dilution

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Published Applications

FCSee 2 publications below

Product Information

PE-65181 targets CD146 in FC applications and shows reactivity with Rabbit, Canine, Mouse, Human samples.

Tested Reactivity Rabbit, Canine, Mouse, Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Immunogen cultured human umbilical cells
Full Name melanoma cell adhesion molecule
Calculated molecular weight 646 aa, 72 kDa
GenBank accession numberBC056418
Gene symbol MCAM
Gene ID (NCBI) 4162
RRIDAB_2883952
Conjugate PE Fluorescent Dye
Excitation/Emission maxima wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification Method The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Storage Buffer PBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988).

Protocols

Product Specific Protocols
FC protocol for PE CD146 antibody PE-65181Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanFC

J Dent Sci

N-acetylcysteine promotes cyclic mechanical stress-induced osteogenic differentiation of periodontal ligament stem cells by down-regulating Nrf2 expression.

Authors - Xun Xi
humanFC

J Tissue Eng

Enhanced effects of antagomiR-3074-3p-conjugated PEI-AuNPs on the odontogenic differentiation by targeting FKBP9

Authors - Tao Jiang